1887

Abstract

SUMMARY

Lymphocytic choriomeningitis virus (LCM) and Pichinde virus grew readily and produced cytopathology in MDCK and PK-15 cells. It is known that in these cell lines, the synthesis or function of defective interfering (DI) virus particles is restricted. Survival curves of single MDCK cells infected with low multiplicities of LCM showed one-particle-to-kill kinetics. At high multiplicities of infection, there was a maximum degree of cell-killing, or even a reduction in the amount of cell-killing, depending on how much DI virus was present in a particular standard virus stock. DI LCM virus could completely prevent standard virus from producing c.p.e. in MDCK monolayers with one-particle-to-protect kinetics. It could still prevent killing of the cells when added within a short time after infection with standard virus, but was able to interfere with synthesis of standard virus when added even later. On passage of LCM or Pichinde virus without dilution in MDCK cells, there was no homologous auto-interference. Furthermore, there was only slight interference with the synthesis of standard virus when these cells were pre-treated with DI virus.

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1978-02-01
2022-01-25
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References

  1. Bro-J⌀rgensen K., Volkert M. 1974; Defects in the immune system of mice infected with lymphocytic choriomeningitis virus. Infection and Immunity 9:605–614
    [Google Scholar]
  2. Dutko F. J., Wright E. A., Pfau C. J. 1976; The RNAs of defective interfering Pichinde virus. Journal of General Virology 31:417–427
    [Google Scholar]
  3. Help G. L., Leon M. E., Coto C. E. 1976; Interferencia asociada a cultivos celulares cronicamente infectados con virus Junin. Revista de la Association Argentina de Microbiologia 8:45–53
    [Google Scholar]
  4. Hoffsten P. E., Dixon F. J. 1973; Effect of immunosuppression on chronic LCM infection of mice. Journal of Experimental Medicine 138:887–899
    [Google Scholar]
  5. Hotchin J. E., Cinits M. 1958; Lymphocytic choriomeningitis infection of mice as a model for the study of latent virus infection. Canadian Journal of Microbiology 4:149–163
    [Google Scholar]
  6. Huang A. S., Baltimore D. 1977; Defective interfering animal viruses. In Comprehensive Virology vol 10 pp 73–116 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
  7. Krug R. M. 1971; Influenza viral RNPs newly synthesized during the latent period of viral growth in MDCK cells. Virology 44:125–136
    [Google Scholar]
  8. Lehmann-Grube F. 1971; Lymphocytic choriomeningitis virus. In Virology Monographs vol 10 pp 23–26 Edited by Gard S., Hallauer C., Mayer K. F. Wien/New York: Springer-Verlag;
    [Google Scholar]
  9. Logan J. C., Fox M. P., Morgan J. H., Makohon A. M., Pfau C. J. 1975; Arenavirus inactivation on contact with N-substituted isatin beta-thiosemicarbazones and certain cations. Journal of General Virology 28:271–283
    [Google Scholar]
  10. Marcus P. L., Sekellick M. J. 1974; Cell-killing by viruses. I. Comparison of cell-killing, plaque-forming, and defective interfering particles of vesicular stomatitis virus. Virology 57:321–338
    [Google Scholar]
  11. Perrault J., Holland J. J. 1972; Variability of vesicular stomatitis vims autointerference with different host cells and virus serotypes. Virology 50:148–158
    [Google Scholar]
  12. Pfau C. J. 1977; The role of defective interfering (DI) virus in arenavirus infections. Medicina, Buenos Aires (in the press)
    [Google Scholar]
  13. Pfau C. J., Welsh R. M., Trowbridge R. S. 1973; Plaque assays and current concepts of regulation in arenavirus infections. In Lymphocytic Choriomeningitis Virus and Other Arenaviruses pp 101–111 Edited by Lehmann-Grube F. Berlin: Springer-Verlag;
    [Google Scholar]
  14. Pfau C. J., Bergold G. H., Casals J., Johnson K. M., Murphy F. A., Pederson I. R., Rawls W. E., Rowe W. P., Webb P. A., Weissenbacher M. C. 1974; Arenaviruses. Intervirology 4:207–213
    [Google Scholar]
  15. Popescu M., Lehmann-Grube F. 1977; Defective interfering particles in mice infected with lymphocytic choriomeningitis virus. Virology 77:78–83
    [Google Scholar]
  16. Popescu M., Schaefer H., Lehmann-Grube F. 1976; Homologous interference of lymphocytic choriomeningitis virus: detection and measurement of interference focus-forming units. Journal of Virology 20:1–8
    [Google Scholar]
  17. Pulkkinen A. J., Pfau C. J. 1970; Plaque size heterogeneity: a genetic trait of lymphocytic choriomeningitis virus. Applied Microbiology 20:123–128
    [Google Scholar]
  18. Staneck L. D., Pfau C. J. 1974; Interfering particles from a culture persistently infected with Parana virus. Journal of General Virology 22:437–440
    [Google Scholar]
  19. Staneck L. D., Welsh R. M., Trowbridge R. S., Wright E. A., Pfau C. J. 1972; Arenaviruses: cellular response to long term in vitro infection with Parana and LCM viruses. Infection and Immunity 6:444–450
    [Google Scholar]
  20. Trapido H., Sanmartin C. 1971; Pichinde: a new virus of the Tacaribe group from Columbia. American Journal of Tropical Medicine and Hygiene 20:631–641
    [Google Scholar]
  21. Traub E. 1961; Interference with Eastern equine encephalomyelitis (EEE) virus in the brains of mice immune to lymphocytic choriomeningitis (LCM). Archiv fur die gesamte Virusforschung 11:419–427
    [Google Scholar]
  22. Volkert M., Lundstedt C. 1971; Tolerance and immunity to the lymphocytic choriomeningitis virus. Annals of the New York Academy of Sciences 181:183–195
    [Google Scholar]
  23. Volkert M., Larsen J. H., Pfau C. J. 1964; Studies on immunological tolerance to LCM virus. 4. The question of immunity in adoptively immunized virus carriers. Acta Pathologica et Microbiologica Scandinavica 61:262–282
    [Google Scholar]
  24. Wagner R. R., Snvder R. M. 1962; Viral interference induced in mice by acute or persistent infection with the virus of lymphocytic choriomeningitis. Nature, London 196:393–394
    [Google Scholar]
  25. Webb H. E., Molomut N., Padnos M., Wetherley-Mein G. 1975; The treatment of 18 cases of malignant disease with an arenavirus. Clinical Oncology 1:157–169
    [Google Scholar]
  26. Welsh R. M. 1971; Lymphocytic choriomeningitis virus defective interfering particles. Ph.D. thesis University of Massachusetts; Amherst, Massachusetts:
    [Google Scholar]
  27. Welsh R. M., Burner P. A., Holland J. J., Oldstone M. B. A., Thompson H. A., Villareal L. P. 1975; A comparison of biochemical and biological properties of standard and defective lymphocytic choriomeningitis virus. Bulletin of the World Health Organization 52:403–408
    [Google Scholar]
  28. Welsh R. M., O’connell C. M., Pfau C. J. 1972; Properties of defective lymphocytic choriomeningitis virus. Journal of General Virology 17:355–359
    [Google Scholar]
  29. Welsh R. M., Pfau C. J. 1972; Determinants of lymphocytic choriomeningitis interference. Journal of General Virology 14:177–187
    [Google Scholar]
  30. Zgorniak-Nowosielska I., Sedwick W. D., Hummeler K., Koprowski H. 1967; New assay procedure for separation of mycoplasmas from virus pools and tissue culture systems. Journal of Virology 1:1227–1237
    [Google Scholar]
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