RT Journal Article SR Electronic(1) A1 Newman, J. F. E. A1 Brown, F.YR 1978 T1 Further Physicochemical Characterization of Nodamura Virus. Evidence that the Divided Genome Occurs in a Single Component JF Journal of General Virology, VO 38 IS 1 SP 83 OP 95 DO https://doi.org/10.1099/0022-1317-38-1-83 PB Microbiology Society, SN 1465-2099, AB SUMMARY Nodamura virus, a small non-enveloped RNA virus, contains two species of RNA sedimenting at 22S (RNA-1) and 15S (RNA-2), a single major polypeptide of mol. wt. 40 × 103 and two minor polypeptides, of mol. wt. 38 and 43 × 103. Evidence is presented that the two RNA species are in the same particle. Although extraction of the virus with SDS-phenol yields the two species of RNA as separate entities, gentle treatment of the virus with guanidine and low concentrations of SDS releases the RNA as a 27S component which contains both RNA-1 and RNA-2 together with a trace of protein. It seems likely that the two RNA species replicate separately because double stranded molecules corresponding to the single stranded RNA-1 and RNA-2 molecules were present in BHK cells infected with the virus., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-38-1-83