A combinatorial analysis of the products of DNA cleavage by restriction endonucleases permits an estimation of the sequence homology between closely related linear DNAs. The main assumption to be made is that fragments of identical length represent equivalent sections of the genomes. All patterns of common and varying restriction sites compatible with the results of the electrophoretic analysis of fragment sizes can be enumerated, each yielding an estimate of the proportion of sequences of restriction site length common to both DNAs. From this, sequence homology is derived by using a model of randomly distributed point mutations relating the two genomes. Possible limitations of the approach are discussed.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error