1887

Abstract

SUMMARY

Ultraviolet (u.v.) light-irradiation of human cytomegalovirus (HCMV) resulted in differential inactivation of virus capacities, e.g. induction of cell rounding, early antigens (EA), nuclear inclusion, HCMV DNA synthesis, cellular DNA synthesis, HCMV-specific DNA polymerase, cellular DNA polymerases and plaque production, while the capacity of HCMV to penetrate cell nuclei was not critically impaired. These results indicated that the virus-coded functions expressed after infection were responsible for all these events except for HCMV-induced stimulation of cellular RNA synthesis which was enhanced by irradiation of the virus at a low dose of u.v. light (6600 ergs/mm). In these experiments phosphonoacetic acid was effectively utilized to detect EA formation by immunofluorescent staining and to differentiate cellular DNA synthesis from virus DNA synthesis.

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1978-01-01
2024-04-24
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References

  1. Aaronson S. A., Lytle C. D. 1970; Decreased host cell reactivation of irradiated SV40 virus in xeroderma pigmentosum. Nature, London 228:359–361
    [Google Scholar]
  2. Albrecht T., Nachtioal M., St. Jeor S. C., Rapp F. 1976; Induction of cellular DNA synthesis and increased mitotic activity in Syrian hamster embryo cells abortively infected with human cytomegalovirus. Journal of General Virology 30:167–177
    [Google Scholar]
  3. Bräutigam A. R., Sauerbier W. 1974; Transcription unit mapping in bacteriophage T7II. Proportionality of number of gene copies, mRNA, and gene product. Journal of Virology 13:1110–1117
    [Google Scholar]
  4. Demarchi J. M., Kaplan A. S. 1976; Replication of human cytomegalovirus DNA: lack of dependence on cell DNA synthesis. Journal of Virology 18:1063–1070
    [Google Scholar]
  5. Furukawa T., Floretti A., Plotkin S. 1973; Growth characteristic of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. Journal of Virology II:991–997
    [Google Scholar]
  6. Garen A., Sinder N. D. 1955; Radiological evidence for partial genetic homology between bacteriophage and host bacteria. Virology I:347–376
    [Google Scholar]
  7. Geder L. 1976; Evidence for early nuclear antigens in cytomegalovirus‒infected cells. Journal of General Virology 32:315–319
    [Google Scholar]
  8. Giraldo G., Beth E., Hammerling U., Tarro G., Kourilsky F. M. 1977; Detection of early antigens in nuclei of cells infected with cytomegalovirus or herpes simplex virus type 1 and 2 by anticomplement immunofluorescence, and use of a blocking assay to demonstrate their specificity. International Journal of Cancer 19:107–116
    [Google Scholar]
  9. Hirai K., Defendi V. 1971; Homology between SV40 DNA and DNA of normal and SV40 transformed Chinese hamster cells. Biochemical and Biophysical Research Communications 42:714–722
    [Google Scholar]
  10. Hirai K., Furukawa T., Plotkin S. A. 1976; Induction of DNA polymerase in WI‒38 and guinea pig cells infected with human cytomegalovirus (HCMV). Virology 70:251–255
    [Google Scholar]
  11. Hirai K., Robb J. A., Defendi V. 1974; Integration of SV40 and induction of cellular DNA synthesis after a ts SV40 infection. Virology 59:266–274
    [Google Scholar]
  12. Hirai K., Watanabe Y. 1976; Induction of a‒type DNA polymerases in human cytomegalovirus‒infected WI‒38 cells. Biochimica et Biophysica Acta 447:328–339
    [Google Scholar]
  13. Honess R. W., Roizman B. 1974; Regulation of herpesvirus macromolecular synthesis I. Cascade regulation of the synthesis of three groups of viral proteins. Journal of Virology 14:8–19
    [Google Scholar]
  14. Huang E.-S. 1975a; Human cytomegalovirus. III. Virus‒induced DNA polymerase. Journal of Virology 16:298–310
    [Google Scholar]
  15. Huang E.-S. 1975b; Human cytomegalovirus. IV. Specific inhibition of virus‒induced DNA polymerase activity and viral DNA replication by phosphonoacetic acid. Journal of Virology 16:1560–1565
    [Google Scholar]
  16. Huang E.-S., Chen S. T., Pagano J. S. 1973; Human cytomegalovirus I. Purification and characterization of viral DNA. Journal of Virology 12:1473–1481
    [Google Scholar]
  17. Jacob F., Wollman E. L. 1955; Etude genetique d’un bacteriophage tempere D‒Escherichia coli. III. Effect du rayonnement ultraviolet sur la recombinaison genetique. Annales de VInstitut Pasteur de Lille 88:724–749
    [Google Scholar]
  18. Keller R., Peitchel R., Goldman J. N., Goldman M. 1976; An IgG‒Fc receptor induced in cytomegalovirus‒infected human fibroblasts. Journal of Immunology 116:772–777
    [Google Scholar]
  19. Penman S. 1966; RNA metabolism in the HeLa cell nucleus. Journal of Molecular Biology 17:117–130
    [Google Scholar]
  20. Plummer G., Goodheart C. R., Henson D., Bowling C. P. 1969; A comparative study of the DNA density and behaviour in tissue cultures of fourteen different herpesviruses. Virology 39:134–137
    [Google Scholar]
  21. Rauth A. M. 1967; Evidence for dark‒reactivation of ultraviolet light damage in mouse L cells. Radiation Research 31:121–138
    [Google Scholar]
  22. Seemayer N. H., Defendi V. 1974; Analysis of minimal functions of simian virus 40. III. Evidence for ‘host cell repair’ of oncogenicity and infectivity of UV‒irradiated simian virus 40. Journal of Virology 13:36–41
    [Google Scholar]
  23. Smith J. D., De Harven E. 1973; Herpes simplex virus and human cytomegalovirus replication in WI38 cells. I. Sequence of viral replication. Journal of Virology 12:919–930
    [Google Scholar]
  24. St. Jeor S. C., Albrecht T. B., Funk F. D., Rapp F. 1974; Stimulation of cellular DNA synthesis by human cytomegalovirus. Journal of Virology 13:353–362
    [Google Scholar]
  25. Tanaka S., Furukawa T., Plotkin S. A. 1975; Human cytomegalovirus stimulates host RNA synthesis. Journal of Virology 15:297–304
    [Google Scholar]
  26. The T. H., Klein G., Langenhuysen M. M. A. C. 1974; Antibody reactions to virus‒specific early antigens (EA) in patients with cytomegalovirus (CMV) infection. Clinical and Experimental Immunology 16:1–12
    [Google Scholar]
  27. Yamamoto H., Shimojo H. 1971; Inactivation of T antigen‒forming capacities of simian virus 40 and adenovirus 12 by ultraviolet irradiation. Journal of Virology 7:419–425
    [Google Scholar]
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