1887

Abstract

SUMMARY

Human peripheral lymphocytes were separated by the use of neuraminidase-treated sheep red blood cells into a population containing < 2% T cells (population B) and a population containing < 2% B cells (population T). Population T was able to replicate herpes simplex virus (HSV) after pre-stimulation with the T cell mitogens phytohaemagglutinin and concanavalin A, while population B was not. HSV also did not replicate in population B after pre-incubation with pokeweed mitogen. Our data strongly suggest that in human lymphocyte cultures HSV replicates in activated T cells. Since B cells after removal of T cells were not stimulated by pokeweed mitogen, it is not clear whether human peripheral blood B cells are unable to replicate HSV or whether they may do so only after adequate stimulation.

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/content/journal/jgv/10.1099/0022-1317-37-3-647
1977-12-01
2022-01-20
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References

  1. Bouroncle B. A., Clausen K. P., Darner E. M. 1970; Replication of herpes simplex virus in cultures of phytohemagglutinin-stimulated human lymphocytes. Journal of the National Cancer Institute 44:1065–1078
    [Google Scholar]
  2. Edelman R., Wheelock E. F. 1968; Specific role of each human leukocyte type in viral infections. II Phytohemagglutinin-treated lymphocytes as host cells for vesicular stomatitis virus replication in vitro. Journal of Virology 2:440–448
    [Google Scholar]
  3. Greaves M., Janossy G., Doenhoff M. 1974; Selective triggering of human T and B lymphocytes in vitro by polyclonal mitogens. Journal of Experimental Medicine 140:1–18
    [Google Scholar]
  4. Heine J. W., Adler W. H. 1976; The kinetics of interferon production by mouse lymphocytes and its modulating effect on the virus plaque forming cell assay as a quantitative method to determine activated lymphocytes. Journal of Immunology 117:1045–1053
    [Google Scholar]
  5. Kirchner H., Brunner H., Ruhl H. 1977; Effect of A. laidlawii on murine and human lymphocyte cultures. Clinical and Experimental Immunology 29:176–180
    [Google Scholar]
  6. Kirchner H., Hirt H. M., Kleinicke C., Munk K. 1976; Replication of herpes simplex virus in mouse spleen cell cultures stimulated by lipopolysaccharide. Journal of Immunology 117:1753–1756
    [Google Scholar]
  7. Lambriex M., Van Der Veen J. 1976; Comparison of replication of adenovirus type 2 and type 4 in human lymphocyte cultures. Infection and Immunity 14:618–622
    [Google Scholar]
  8. Miller G., Enders J. F. 1968; Vaccinia virus replication and cytopathic effect in cultures of phytohemagglutinin-treated human peripheral blood leukocytes. Journal of Virology 2:787–792
    [Google Scholar]
  9. Nahmias A. J., Kibrick S., Rosan R. C. 1964; Viral leukocyte interrelationships. I. Multiplication of a DNA virus-herpes simplex-in human leukocyte cultures. Journal of Immunology 93:69–74
    [Google Scholar]
  10. Ringden O. 1976; Activation of human lymphocyte subpopulations by rabbit anti-human β2-micro-globulin and by lipopolysaccharide. Scandinavian Journal of Immunology 5:891–900
    [Google Scholar]
  11. Sullivan J. L., Barry D. W., Lucas S. J., Albrecht P. 1975; Measles infection of human mononuclear cells. I. Acute infection of peripheral blood lymphocytes and monocytes. Journal of Experimental Medicine 142:773–784
    [Google Scholar]
  12. Wheelock E. F. 1965; Interferon-like virus-inhibitor induced in human leukocytes by photohemagglutinin. Science 149:310–311
    [Google Scholar]
  13. Willems F. TH. C., Vondrovicova E. L. 1970; Replication of poliovirus in human lymphocytes stimulated with specific antigens. Archiv für die gesamte Virusforschung 30:31–38
    [Google Scholar]
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