1887

Abstract

SUMMARY

The neuraminidase of B/Lee/40 influenza virus was isolated following detergent dissociation or tryptic digestion of virus particles and the amino acid and carbohydrate compositions of the two preparations are reported.

The results indicate that the carbohydrate side-chains of the neuraminidase contain only -acetylglucosamine, galactose, mannose and fucose, that they are attached by -acetylglucosamine-asparagine linkages, and that the molecular weights of the neuraminidase subunit and the intact molecule are about 70000 and 280000, respectively. The results also suggest that more than 50% of the carbohydrate is attached to the membrane-associated ‘stalk’ of the molecule and that in this ‘stalk’ region the subunits are linked by disulphide bonds.

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/content/journal/jgv/10.1099/0022-1317-37-3-625
1977-12-01
2024-11-02
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