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The native and denatured 60 to 70S RNAs of two murine oncornaviruses and one simian oncornavirus were examined for their poly (A) content. In the native genome, a significant proportion of poly (A)− RNA was found and intact 60 to 70S RNA complex was identified in this fraction. The amount of poly (A)− RNA in the native genome was related to cellular growth and seemed to be independent of virus maturation. The subunits obtained after thermal denaturation consisted of approx. 2/3 poly (A)+ and 1/3 poly (A)− RNA.
The poly (A)− subunits were mainly composed of 20 to 28S RNA and the poly (A)+ subunits of 30 to 35S RNA. The results of competitive molecular hybridization of these two fractions with virus cDNA suggested that the two species of subunits possessed similar nucleotide sequences.
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