Treatment of a fully permissive monkey kidney cell clone (CVCl) with 5-iodo-2′-deoxyuridine (IdUrd) before infection with SV40 virus enhances the yield of virions 10- to 50-fold. The increase is detectable only after slowing down the virus growth cycle by reducing the m.o.i. and by incubating at low temperature. The IdUrd pre-treatment enhances SV40 DNA replication and the number of V-antigen and virus-synthesizing cells. The potentiating effect of IdUrd is not observed when the pre-treated cells are infected with SV40 DNA. The synthesis of SV40 T-antigen is increased even in the presence of cytosine arabinoside (Ara-C). IdUrd inhibits cellular DNA synthesis but the incorporation of H-uridine and H-leucine into RNA and proteins is not affected. Late virus functions are preferentially expressed in the cells in which cellular DNA synthesis is inhibited. The results suggest that the enhancement by IdUrd of SV40 replication would be the consequence of at least two complementary events: (1) stimulation of an early virus function localized between the arrival of the virus DNA in the nucleus and T-antigen induction; (2) inhibition of cellular DNA synthesis with a consequent greater availability of cellular factors required for virus growth.


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