1887

Abstract

SUMMARY

Treatment of a fully permissive monkey kidney cell clone (CVCl) with 5-iodo-2′-deoxyuridine (IdUrd) before infection with SV40 virus enhances the yield of virions 10- to 50-fold. The increase is detectable only after slowing down the virus growth cycle by reducing the m.o.i. and by incubating at low temperature. The IdUrd pre-treatment enhances SV40 DNA replication and the number of V-antigen and virus-synthesizing cells. The potentiating effect of IdUrd is not observed when the pre-treated cells are infected with SV40 DNA. The synthesis of SV40 T-antigen is increased even in the presence of cytosine arabinoside (Ara-C). IdUrd inhibits cellular DNA synthesis but the incorporation of H-uridine and H-leucine into RNA and proteins is not affected. Late virus functions are preferentially expressed in the cells in which cellular DNA synthesis is inhibited. The results suggest that the enhancement by IdUrd of SV40 replication would be the consequence of at least two complementary events: (1) stimulation of an early virus function localized between the arrival of the virus DNA in the nucleus and T-antigen induction; (2) inhibition of cellular DNA synthesis with a consequent greater availability of cellular factors required for virus growth.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-37-3-569
1977-12-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/37/3/JV0370030569.html?itemId=/content/journal/jgv/10.1099/0022-1317-37-3-569&mimeType=html&fmt=ahah

References

  1. Barbanti-Brodano G., Swetly P., Koprowski H. 1970; Early events in the infection of permissive cells with Simian virus 40: adsorption, penetration and uncoating. Journal of Virology 6:78–86
    [Google Scholar]
  2. Black P., Crawford L. V. 1964; The purification of Simian Virus 40. Virology 24:381–387
    [Google Scholar]
  3. De Marchi J. M., Kaplan A. S. 1976; Replication of human cytomegalovirus DNA: lack of dependence on cell DNA synthesis. Journal of Virology 18:1063–1070
    [Google Scholar]
  4. Graessmann A., graessmann M., Mueller C. 1976; Regulatory mechanisms of Simian Virus 40 gene expression in permissive and non-permissive cells. Journal of Virology 17:854–858
    [Google Scholar]
  5. Green J. A., Baron S. 1975; 5-Iododeoxyuridine potentiation of the replication in vitro of several unrelated RNA and DNA viruses. Science 190:1099–1101
    [Google Scholar]
  6. Hirt B. 1967; Selective extraction of polyoma DNA from infected mouse cell cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  7. Jerkofsky M., Rapp F. 1975; Stimulation of adenovirus replication in simian cells in the absence of a helper virus by pretreatment of the cells with iododeoxyuridine. Journal of Virology 15:253–258
    [Google Scholar]
  8. Lavialle Ch., Morris A. G., Suárez H. G., Estrade S., Stévenet J., Cassingena R. 1977; Simian virus 40 -Chinese hamster kidney cell interaction. IV. Enhanced virus replication in infected cells upon treatment with mitomycin C. Journal of General Virology 36:137–149
    [Google Scholar]
  9. Manteuil S., Pagés J., Stéhélin D., Girard M. 1973; Replication of simian virus 40 deoxyribonucleic acid: analysis of the one step growth cycle. Journal of Virology 11:98–106
    [Google Scholar]
  10. May E., May P., Weill R. 1971; Analysis of the events leading to SV40-induced chromosome replication and mitosis in primary mouse kidney cell cultures. Proceedings of the National Academy of Sciences of the United States of America 68:1208–1211
    [Google Scholar]
  11. Paul N. R., Iwakata S., Rhodes A. J., Labzoffsky N. A. 1974; Enhancing effect of halogenated pyrimidines (BUdR and IUdR) on the growth of rubella virus in BHK-21 cells. Archiv für die gesamte Virusforschung 44:144–146
    [Google Scholar]
  12. Plummer G., Goodheart C. R. 1974; Growth of murine cytomegalovirus in a heterologous cell system and its enhancement by 5-iodo-2-deoxyuridine. Infection and Immunity 10:251–256
    [Google Scholar]
  13. Reed S. I., Fergusson J., Davis R. W., Stark G. R. 1975; T-antigen binds to simian virus 40 DNA at the origin of replication. Proceedings of the National Academy of Sciences of the United States of America 72:1605–1609
    [Google Scholar]
  14. Robb J. A., Tegtmeyer P., Ishikawa A., Ozer H. L. 1974; Antigenic phenotypes and complementation groups of temperature-sensitive mutants of Simian Virus 40. Journal of Virology 13:662–665
    [Google Scholar]
  15. Speers W. C., Lehman J. M. 1976; Increased susceptibility of murine teratocarcinoma cells to simian virus 40 and polyoma virus following treatment with 5-bromodeoxyuridine. Journal of Cellular Physiology 88:297–306
    [Google Scholar]
  16. Staal S. P., Rowe W. P. 1975; Enhancement of adenovirus infection in WI-38 and AGMK cells by pretreatment of cells with 5-iodo-2′-deoxyuridine. Virology 64:513–519
    [Google Scholar]
  17. St Jeor S., Rapp F. 1973a; Cytomegalovirus replication in cells pretreated with 5-iodo-2-deoxyuridine. Journal of Virology 11:986–990
    [Google Scholar]
  18. St Jeor S., Rapp F. 1973b; Cytomegalovirus conversion of non-permissive cells to a permissive state for virus replication. Science 181:1060–1061
    [Google Scholar]
  19. Suárez H. G., Cassingena R., Estrade S., Wicker R., Lavialle CH., Lazar P. 1974; Properties of SV40 rescued from actinomycin D-sensitive and actinomycin D-resistant transformed hamster cells. I. Lytic infection. Archiv für die gesamte Virusforschung 46:93–104
    [Google Scholar]
  20. Suárez H. G., Morris A. G., Lavialle Ch., Cassingena R. 1976; Enhanced SV40-virus replication in Chinese hamster kidney cells pretreated with 5-iodo-2′-deoxyuridine. Archives of Virology 50:249–253
    [Google Scholar]
  21. Tegtmeyer P., Schwartz M., Collins J. K., Rundell K. 1975; Regulation of tumor antigen synthesis by simian virus 40 gene A. Journal of Virology 16:168–178
    [Google Scholar]
  22. Tournier P., Cassingena R., Wicker R., Coppey J., Suárez H. G. 1967; Etude du mécanisme de l’induction chez les cellules de hamster syrien transformées par le virus SV40. I. Propriétés d’une lignée celluiaire clonale. International Journal of Cancer 2:117–132
    [Google Scholar]
  23. Wicker R., Avrameas S. 1969; Localization of virus antigens by enzyme-labelled antibodies. Journal of General Virology 4:465–471
    [Google Scholar]
  24. Yamamoto K., Yamaguchi N., Oda K. 1975; Mechanism of interferon-induced inhibition of early simian virus 40 (SV40) functions. Virology 68:58–70
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-37-3-569
Loading
/content/journal/jgv/10.1099/0022-1317-37-3-569
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error