1887

Abstract

Summary

Neuraminidase activity could be demonstrated in highly concentrated preparations of human parainfluenza 1 virus, strain C 35 (HA2 virus). Among the substrates used, the most suitable are -acetyl neuramin lactose and fetuin. Mucin type I and type II were not hydrolysed. The neuraminidase exhibited some characteristics similar to those of the other paramyxoviruses (Sendai, NDV, mumps, human parainfluenza 2 virus): the optimum pH ranged between 5 and 5.4, and the value was 5 × 10 when tested with -acetyl neuramin lactose. Its optimum activity was between 37 and 40 °C and it was thermolabile, the enzymic activity being reduced to 50% in 5 min at 45 °C and entirely destroyed at 50 °C in the same period. The thermal inactivation constants of neuraminidase and haemagglutinin and the temperature which inactivated 50% of both these activities were very similar to those already shown for NDV. Haemagglutinin and neuraminidase activities were rapidly destroyed by ionic detergents, but not by non-ionic detergents.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-37-3-547
1977-12-01
2019-11-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-37-3-547
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error