1887

Abstract

SUMMARY

Neuraminidase activity could be demonstrated in highly concentrated preparations of human parainfluenza 1 virus, strain C 35 (HA2 virus). Among the substrates used, the most suitable are -acetyl neuramin lactose and fetuin. Mucin type I and type II were not hydrolysed. The neuraminidase exhibited some characteristics similar to those of the other paramyxoviruses (Sendai, NDV, mumps, human parainfluenza 2 virus): the optimum pH ranged between 5 and 5.4, and the value was 5 × 10 when tested with -acetyl neuramin lactose. Its optimum activity was between 37 and 40 °C and it was thermolabile, the enzymic activity being reduced to 50% in 5 min at 45 °C and entirely destroyed at 50 °C in the same period. The thermal inactivation constants of neuraminidase and haemagglutinin and the temperature which inactivated 50% of both these activities were very similar to those already shown for NDV. Haemagglutinin and neuraminidase activities were rapidly destroyed by ionic detergents, but not by non-ionic detergents.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-37-3-547
1977-12-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/37/3/JV0370030547.html?itemId=/content/journal/jgv/10.1099/0022-1317-37-3-547&mimeType=html&fmt=ahah

References

  1. Alexander D. J. 1974; Comparison of the neuraminidases of three avian paramyxoviruses. Archiv für die gesamte Vimsforschung 44:28–34
    [Google Scholar]
  2. Aymard-Henry M., Coleman M. T., Dowdle W. R., Laver W. G., Schild G. C., Webster R. G. 1973; Influenza virus neuraminidase and neuraminidase inhibition test procedures. World Health Organization Bulletin 48:199–202
    [Google Scholar]
  3. Brostrom M. A., Bruening G., Bankowski R. A. 1971; Comparison of neuraminidases of paramyxoviruses with immunologically dissimilar haemagglutinins. Virology 46:856–865
    [Google Scholar]
  4. Chanock R. M., Parrott R. H., Bell J. A., Rowe W. P., Huebner R. J. 1958; New viruses observed in children with respiratory diseases. Public Health Report 73:193–195
    [Google Scholar]
  5. Darrell R. W., Howe C. 1964; The neuraminidase of parainfluenza virus (type 2). Proceedings of the Society for Experimental Biology and Medicine 116:1091–1094
    [Google Scholar]
  6. Drzeniek R. 1972; Viral and bacterial neuraminidases. Current topics in Microbiology and Immunology 59:35–74
    [Google Scholar]
  7. Drzeniek R. 1973; Substrate specificity of neuraminidases. Histochemical Journal 5:271–290
    [Google Scholar]
  8. Drzeniek R., Bogel K., Rott R. 1967; On the classification of bovine parainfluenza 3 viruses. Virology 31:725–727
    [Google Scholar]
  9. Drzeniek R., Seto J. T., Rott R. 1966; Characterization of neuraminidases from Myxoviruses. Biochimica et Biophysica Acta 128:547–558
    [Google Scholar]
  10. Famulari N. G., Fleissner E. 1976; High titre replication of non-defective Sendai virus in MDBK cells. Journal of Virology 17:597–604
    [Google Scholar]
  11. Han R. G., Puck T. T. 1962; Quantitative growth of isolated mammalian cells. Methods in Enzymology V90–119 Edited by Colowick S. P., Kaplan N. O. New York & London: Academic Press;
    [Google Scholar]
  12. Homma M., Ohuchi M. 1973; Trypsin action on the growth of Sendai virus in tissue culture cells. Ⅲ. Structural differences of Sendai viruses grown in eggs and tissue culture cells. Journal of Virology 13:788–800
    [Google Scholar]
  13. Iinuma M., Yoshida T., Nagai Y., Maeno K., Matsumoto K., Hoshino M. 1971; Subunits of NDV. Haemagglutinin and neuraminidase subunits of Newcastle Disease virus. Virology 46:663–677
    [Google Scholar]
  14. Ishida N., Homma M., Osato T., Iinuma Y., Miyamoto T. 1964; Persistent infection in HeLa cells with haemadsorption virus type 2. Virology 39:277–285
    [Google Scholar]
  15. Jensik J. C., Silver S. 1976; Polypeptides of mumps virus. Journal of Virology 17:363–373
    [Google Scholar]
  16. Leprat R., Aymard M., Bardeletti G. 1975; Contribution to the study of the mumps neuraminidase. International Virology 3: 3rd International Congress for Virology, Madrid 10 to 17 September 1975, C333
    [Google Scholar]
  17. Lief F. S., Loh W., Ter Meulen V., Koprowski H. 1975; Antigenic variation among parainfluenza type 1 (Sendai) viruses: analysis of 6/94 virus. Intervirology 5:1–9
    [Google Scholar]
  18. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  19. Maeno K., Yoshida T., Iinuma M., Nagai Y., Matsumoto T., Asai J. 1970; Isolation of haemagglutinin and neuraminidase subunits of haemagglutinating virus of Japan. Journal of Virology 6:492–499
    [Google Scholar]
  20. Mountcastle W. E., Compans R. W., Choppin P. W. 1971; Proteins and glycoproteins of paramyxoviruses: a comparison of simian virus 5, NDV and Sendai virus. Journal of Virology 7:47–52
    [Google Scholar]
  21. Nisizawa K., Hashimoto Y. 1970; Glycoside hydrolases and glycosyl transferase. In The Carbohydrates 2nd edition, vol IIa pp 241–300 Edited by Pigman W., Horton D., Herp A. New York & London: Academic Press;
    [Google Scholar]
  22. Phillips P. E., Christian C. L. 1970; Myxovirus antibody increases in human tissue disease. Science 168:982–984
    [Google Scholar]
  23. Pierce J. S., Haywood A. M. 1973; Thermal inactivation of Newcastle Disease virus. I. Coupled inactivation rates of haemagglutinating and neuraminidase activities. Journal of Virology 11:168–176
    [Google Scholar]
  24. Scheid A., Caliguiri L. A., Compans R. W., Choppin P. W. 1972; Isolation of paramyxovirus glycoproteins. Association of both haemagglutinating and neuraminidase activities with the larger SV5 glycoprotein. Virology 50:640–652
    [Google Scholar]
  25. Scheid A., Choppin P. W. 1973; Isolation and purification of the envelope proteins of Newcastle Disease virus. Journal of Virology 11:263–271
    [Google Scholar]
  26. Scheid A., Choppin P. W. 1974; Identification of biological activities of paramyxovirus glycoproteins. Activation of cell fusion, haemolysis and infectivity by proteolytic cleavage of an inactive precursor protein of Sendai virus. Virology 57:475–490
    [Google Scholar]
  27. Spiro R. G. 1964; Periodate oxidation of the glycoprotein fetuin. Journal of Biological Chemistry 239:567–577
    [Google Scholar]
  28. Spiro R. G. 1970; Glycoproteins. Annual Review of Biochemistry 39:599–638
    [Google Scholar]
  29. Tozawa H., Homma M., Ishida N. 1967; Specificity of neuraminidase activity of Sendai virus. Proceedings of the Society for Experimental Biology and Medicine 124:734–739
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-37-3-547
Loading
/content/journal/jgv/10.1099/0022-1317-37-3-547
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error