1887

Abstract

SUMMARY

Extracts from mouse interferon-treated mouse cells (Ehrlich ascites tumour and L929) were reported earlier to differ in several characteristics from extracts of untreated cells. We report now that the effect of treatment with human interferon of cells of a human line (HeLa S) is similarly manifested in the cell extract. A comparison of extracts from interferon-treated HeLa S cells (S30) with that from untreated cells (S30) revealed: (a) an impairment in S30 of the methylation of (unmethylated) reovirus mRNAs by the cellular and virus enzymes; (b) a faster degradation of reovirus mRNAs in S30 but only in reaction mixtures supplemented with double-stranded RNA and ATP; (c) an increased phosphorylation by ATP of one (or two) proteins in S30 but only in reaction mixtures supplemented with double-stranded RNA and (d) a more pronounced inhibition of translation by double-stranded RNA in S30. No such effects were observed in extracts prepared from HeLa cells treated with mouse interferon.

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1977-12-01
2022-10-01
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