1887

Abstract

SUMMARY

Intracytoplasmic A particles can be found in the cytoplasm of mammary tumours, certain leukaemias and Leydig cell tumours of the mouse. These intracytoplasmic A particles share antigenic determinants with the mouse mammary tumour virus (MTV) and are considered to be precursors of MTV B-type particles on the basis of morphological comparison. Both DNA and RNA have been reported to be associated with these intracytoplasmic A particles (Tanaka, Tamura & Tsujimura, 1972; Smith, Litwach & Longfellow, 1974). In this study it is shown that (1) the RNA of intracytoplasmic A particles is homologous to the RNA of MTV; (2) the DNA of the intracytoplasmic A particles is not a replicative form of the MTV genome, because no hybridization was observed between this intracytoplasmic A particle DNA and the radioactively labelled RNA or cDNA of MTV; (3) the intracytoplasmic A particles also contain an RNA-directed DNA polymerase activity with a cation preference which is similar to that of the reverse transcriptase of the MTV in a poly(rC).oligo(dG)-directed DNA polymerase assay.

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1977-12-01
2024-04-20
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References

  1. Benveniste R. E., Todaro G. J., Scolnick E. M., Parks W. P. 1973; Partial transcription of murine type C viral genomes in BALB/c cell lines. Journal of Virology 12:711–720
    [Google Scholar]
  2. Birnstiel M. L., Sells B. H., Purdom J. F. 1972; Kinetic complexity of RNA molecules. Journal of Molecular Biology 63:21–39
    [Google Scholar]
  3. Britten D. E., Graham R., Neufeld B. R. 1974; Analysis of repeating DNA sequences by reassociation. Methods of Enzymology 29:363–419
    [Google Scholar]
  4. Dickson C., Skehel J. 1974; The polypeptide composition of mouse mammary tumor virus. Virology 58:387–395
    [Google Scholar]
  5. Dion A., Vaydya A. B., Fout C. S. 1974; Cation p references for poly rC: oligo dG directed DNA synthesis by RNA tumor viruses and human milk particulates. Cancer Research 34:3509–3515
    [Google Scholar]
  6. Gallo R. C., Gallagher R. E., Miller N. R., Mondel H., Saringer W. C., Ayer R. J., Smith R. G., Gilles-Pie D. 1974; Relationship between components in primate RNA tumor viruses and in the cytoplasm of human leukemic cells: implication to leukemogenesis. Cold Spring Harbor Symposium Quantitative Biology 39:933–961
    [Google Scholar]
  7. Glisin V., Crkvenjakov R., Buys C. 1974; Ribonucleic acid isolation by cesium chloride centrifugation. Biochemistry 13:2633–2637
    [Google Scholar]
  8. Longfellow D. G., Smith G. H. 1976; Further characterization of intracytoplasmic A particle-associated DNA. Journal of Virology 17:816–823
    [Google Scholar]
  9. Marcus S. L., Sarkar N. H., Modak M. J. 1976; Purification and properties of murine mammary tumor virus DNA polymerase. Virology 71:242–254
    [Google Scholar]
  10. Michalides R., Schlom J. 1975; Relationship in nucleic acid sequences between mouse mammary tumor-virus variants. Proceedings of the National Academy of Sciences of the United States of America 11:4635–4639
    [Google Scholar]
  11. Michalides R., Vlahakis G., Schlom J. 1976a; Biochemical approach to the study of the transmission of mouse mammary tumor viruses in mouse strains RIII and C3H. International Journal of Cancer 18:105–115
    [Google Scholar]
  12. Michalides R., Schlom J., Pearson J., Perk K., Dahlberg J. 1976b; Characterization of the oncorna-virus particles in the plasma of guinea pigs with L2C leukemia. Journal of Virology 18:1120–1130
    [Google Scholar]
  13. Nowinski R., Sarkar N., Old L., Moore D. H., Scheer D., Hilgers J. 1971; Characteristics of the structural components of the mouse mammary tumor virus: II. Viral proteins and antigens. Virology 46:21–38
    [Google Scholar]
  14. Sarkar N. H., Dion A. S. 1975; Polypeptides of the mouse mammary tumor virus. I. Characterization of two group-specific antigens. Virology 64:471–491
    [Google Scholar]
  15. Smith G. H., Lee B. K. 1975; Mouse mammary tumor virus polypeptide precursors in intracytoplasmic A particles. Journal of the National Cancer Institute 55:493–496
    [Google Scholar]
  16. Smith G. H., Litwach M., Longfellow D. G. 1974; High molecular weight DNA associated with intracytoplasmic A particle inclusions. Cancer Research 34:2654–2662
    [Google Scholar]
  17. Smith G. H., Longfellow D. G., Nimon J. W. 1975; Autoradiographic evidence for the association of DNA with intracytoplasmic A particles inclusions in situ . Journal of the National Cancer Institute 55:1413–1419
    [Google Scholar]
  18. Smith G. H., Wivel N. A. 1972; Isolation and partial characterization of intracytoplasmic A particles. Virology 48:270–274
    [Google Scholar]
  19. Stuck B., Boyse E. A., Old L. J., Carswell E. A. 1964; ML: a new antigen found in leukemia and mammary tumors of the mouse. Nature, London 203:1033–1034
    [Google Scholar]
  20. Tanaka H., Tamura A., Tsuiimura D. 1972; Properties of the intracytoplasmic A particles purified from mouse tumors. Virology 49:61–78
    [Google Scholar]
  21. Tooze J. 1973; The molecular biology of tumor viruses. Cold Spring Harbor Laboratories Cold Spring Harbor; New York:
    [Google Scholar]
  22. Wunderlich V., Zotter St. Sydow. 1976; RNA dependent DNA polymerase activity in intracytoplasmic type A particles of mouse mammary tumors. Acta biologica medica germanica 35:1741–1744
    [Google Scholar]
  23. Zotter St., Muller M., Kemmer Ch., Johannsen B. A., Grossman H. 1976a; Further evidence for common antigens in intracytoplasmic A particles of mouse mammary tumors and B type virions of murine milk. Experimentelle Pathologie 12:46–54
    [Google Scholar]
  24. Zotter St., Kryukowa I. N., Bukrinskaya A. G., Lezhnewa O. M., Ilyin K. V., Miller G. G., Muller M. 1976b; Presence of the P27 antigenicity and absence of the gp52 antigenecity and leukemia virus anti-gens in intracytoplasmic A particles (1Ap) of mouse mammary tumour origin. Archiv für Geschwulst-forschung 46621–629
    [Google Scholar]
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