1887

Abstract

SUMMARY

Haemagglutinating encephalomyelitis virus (HEV), a member of the coronavirus group, was adapted for growth in adult pig thyroid cell cultures and purified by ammonium sulphate precipitation and rate zonal centrifugation through sucrose gradients. Polyacrylamide gel electrophoresis of samples of purified virus revealed the presence of five polypeptides, four of which contained carbohydrate. The molecular weights of these proteins were 180000 (gp 180), 125000 (gp 125), 100000 (gp 100), 56000 (p 56) and 26 500 (gp 26.5). After treatment of the virus with the non-ionic detergent Nonidet P40, two subviral components were isolated. As RNA-containing particle, sedimenting in sucrose gradients at the same rate as untreated virus, was analysed and found to contain two polypeptides, p 56 and gp 26.5. The second complex sedimented at a much slower rate and contained three glycoproteins gp 180, gp 125 and gp 100. Comparison of these findings with data published for other members of the coronavirus group is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-37-3-487
1977-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/37/3/JV0370030487.html?itemId=/content/journal/jgv/10.1099/0022-1317-37-3-487&mimeType=html&fmt=ahah

References

  1. Alexander T. J. L., Richards W. P. C., Roe C. K. 1959; An encephalomyelitis of suckling pigs in Ontario. Canadian Journal of Comparative Medicine & Veterinary Science 23:316–319
    [Google Scholar]
  2. Almeida J. D., Berry D. M., Cunningham C. H., Hamre D., Hofstad M. S., Mulluci L., Mcintosh K., Tyrrell D. A. J. 1968; Coronaviruses. Nature, London 220:650
    [Google Scholar]
  3. Bingham R. W. 1975; The polypeptide composition of avian infectious bronchitis virus. Archives of Virology 49:207–216
    [Google Scholar]
  4. Bretscher M. S. 1971; Major human erythrocyte glycoproteins span the cell membrane. Nature, New Biology 231:229–232
    [Google Scholar]
  5. Cartwright S. F., Lucas M., Cavill J. P., Gush A. F., Blandford T. B. 1969; Vomiting and wasting disease of piglets. Veterinary Record 84:175–176
    [Google Scholar]
  6. Collins M. S., Alexander D. J., Harkness J. W. 1976; Heterogeneity of infectious bronchitis virus grown in eggs. Archives of Virology 50:55–72
    [Google Scholar]
  7. Garwes D. J., Pocock D. H. 1975; The polypeptide structure of transmissible gastroenteritis virus. Journal of General Virology 29:25–34
    [Google Scholar]
  8. Garwes D. J., Pocock D. H., Pike B. V. 1976; Isolation of subviral components from transmissible gastroenteritis virus. Journal of General Virology 32:283–294
    [Google Scholar]
  9. Garwes D. J., Pocock D. H., Wijaszka T. M. 1975; Identification of heat-dissociable RNA complexes in two porcine coronaviruses. Nature, London 257:508–510
    [Google Scholar]
  10. Gotink W. M., Lambers G. M., van Soest H., van Ulsen F. W. 1969; Vomiting and wasting disease in piglets. Veterinary Record 84:445–446
    [Google Scholar]
  11. Greig A. S., Johnson C. M., Bouillant A. M. P. 1971; Encephalomyelitis of swine caused by a haemagglutinating virus. VI. Morphology of the virus. Research in Veterinary Science 12:305–307
    [Google Scholar]
  12. Greig A. S., Mitchell D., Corner A. H., Bannister G. L., Meads E. B., Julian R. J. 1962; A haemagglutinating virus producing encephalomyelitis in baby pigs. Canadian Journal of Comparative Medicine & Veterinary Science 26:49–56
    [Google Scholar]
  13. Harris T. J. R., Brown F. 1975; Correlation of polypeptide composition with antigenic variation in the swine vesicular disease and Coxsackie B5 viruses. Nature, London 258:758–760
    [Google Scholar]
  14. Hierholzer J. C. 1976; Purification and biophysical properties of human coronavirus 229E. Virology 75:155–165
    [Google Scholar]
  15. Hierholzer J. C., Palmer E. L., Whitfield S. G., Kaye H. S., Dowdle W. R. 1972; Protein composition of coronavirus OC43. Virology 48:516–527
    [Google Scholar]
  16. Kennedy D. A., Johnson-Lussenburg C. M. 1976; Isolation and morphology of the internal component of human coronavirus, strain 229E. Intervirology 6:197–206
    [Google Scholar]
  17. Laskey R. A., Mills A. D. 1975; Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. European Journal of Biochemistry 56:335–341
    [Google Scholar]
  18. Mengeling W. L. 1972a; Precipitating antigens of haemagglutinating encephalomyelitis virus demonstrated by agar gel immunodiffusion. American Journal of Veterinary Research 33:1527–1535
    [Google Scholar]
  19. Mengeling W. L. 1972b; Haemadsorption plaque assay for haemagglutinating encephalomyelitis virus. American Journal of Veterinary Research 33:2075–2080
    [Google Scholar]
  20. Mengeling W. L., Boothe A. D., Ritchie A. E. 1972; Characteristics of a coronavirus (strain 67N) of pigs. American Journal of Veterinary Research 33:297–308
    [Google Scholar]
  21. Pensaert M. B., Callebaut P. E. 1974; Characteristics of a coronavirus causing vomiting and wasting in pigs. Archiv für die gesamte Virusforschung 44:35–50
    [Google Scholar]
  22. Philip J. I. H., Cartwright S. F., Scott S. C. 1971; The size and morphology of TGE and vomiting and wasting disease viruses of pigs. Veterinary Record 88:311–312
    [Google Scholar]
  23. Pocock D. H., Garwes D. J. 1975; The influence of pH on the growth and stability of transmissible gastroenteritis virus in vitro . Archives of Virology 49:239–247
    [Google Scholar]
  24. Richards W. P. C., Savan M. 1960; Viral encephalomyelitis of pigs. A preliminary report on the transmissibility and pathology of a disease observed in Ontario. Cornell Veterinarian 50:132–155
    [Google Scholar]
  25. Sharpee R. L., Mebus C. A., Bass E. P. 1976; Characterization of a calf diarrheal coronavirus. American Journal of Veterinary Research 37:1031–1041
    [Google Scholar]
  26. Tyrrell D. A. J., Almeida J. D., Cunningham C. H., Dowdle W. R., Hofstad M. S., Mcintosh K., Tajima M., Zakstelskaya L. Ya., Easterday B. C., Kapikian A., Bingham R. W. 1975; Coronaviridae. Intervirology 5:76–82
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-37-3-487
Loading
/content/journal/jgv/10.1099/0022-1317-37-3-487
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error