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Volume 37, Issue 2

RNA Synthesis Directed by a Temperature-Sensitive Mutant of Semliki Forest Virus Free

Abstract

Summary

RNA synthesis induced by a temperature-sensitive RNA negative mutant, ts-4, of Semliki Forest virus was studied both at the permissive (28 °C) and restrictive temperature (39 °C). At 28 °C ts-4 directed RNA synthesis was qualitatively similar to the wild type but overall synthesis remained somewhat lower. Only about 3% of RNA was synthesized compared to the wild type virus when ts-4 was maintained at 39 °C throughout the infection. The sedimenting radioactivity was almost exclusively in 42S RNA. When ts-4 infected cultures were shifted from 28 to 39 °C at 5 h post-infection, the synthesis of 26S RNA was shut off while 42S RNA synthesis continued. The synthesis of 26S RNA started again even in the presence of cycloheximide if the cultures were shifted back to 28 °C. Our results suggest that 26S RNA synthesis is controlled by a virus-specific protein, the function of which is not required for the synthesis of 42S RNA, In ts-4 this protein is denatured at 39 °C and becomes non-functional, but renatures and becomes functional upon shift down to 28 °C.

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© Journal of General Virology 1977
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1977-11-01
2024-04-25
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References

  1. Atkins G. J., Samuels J., Kennedy S. I. T. 1974; Isolation and preliminary characterization of temperature-sensitive mutants of Sindbis virus strain AR339. Journal of General Virology 25:371–380
     [Google Scholar]
  2. Bracha M., Leone A., Schlesinger M. J. 1976; Formation of Sindbis virus nonstructural protein and its relation to 42 S mRNA function. Journal of Virology 20:612–620
     [Google Scholar]
  3. Bruton C. J., Kennedy S. I. T. 1975; Semliki Forest virus intracellular RNA: properties of the multistranded RNA species and kinetics of positive and negative strand synthesis. Journal of General Virology 28:612–127
     [Google Scholar]
  4. Burge B. W., Pfefferkorn E. R. 1966; Isolation and characterization of conditional-lethal mutants of Sindbis virus. Virology 30:204–213
     [Google Scholar]
  5. Burge B. W., Pfefferkorn E. R. 1968; Functional defects of temperature-sensitive mutants of Sindbis virus. Journal of Molecular Biology 35:193–205
     [Google Scholar]
  6. Cancedda R., Swanson R., Schlesinger M. J. 1974; Effects of different RNAs and components of the cell-free system on in vitro synthesis of Sindbis viral proteins. Journal of Virology 14:652–663
     [Google Scholar]
  7. Cancedda R., Villa-komaroff L., Lodish H. F., Schlesinger M. 1975; Initiation sites for translation of Sindbis virus 42 S and 26 S messenger RNAs. Cell 6:215–222
     [Google Scholar]
  8. Clegg J. C. S., Kennedy S. I. T. 1975; Translation of Semliki Forest virus intracellular 26 S RNA: characterization of the products synthesised in vitro. European Journal of Biochemistry 53:175–183
     [Google Scholar]
  9. Clegg J. C. S., Brzeski H., Kennedy S. I. T. 1976; RNA polymerase components in Semliki Forest virus-infected cells: synthesis from large precursors. Journal of General Virology 32:413–430
     [Google Scholar]
  10. Clewley J. P., Kennedy S. I. T. 1976; Purification and polypeptide composition of Semliki Forest virus RNA polymerase. Journal of General Virology 32:395–411
     [Google Scholar]
  11. Friedman R. M., Levy H. B., Carter W. B. 1966; Replication of Semliki Forest virus: three forms of viral RNA produced during infection. Proceedings of the National Academy of Sciences of the United States of America 56:440–446
     [Google Scholar]
  12. Glanville N., Morser J., Uomala P., Kääriäinen L. 1976a; Simultaneous translation of structural and nonstructural proteins from Semliki Forest virus RNA in two eukaryotic systems in vitro. European Journal of Biochemistry 64:167–175
     [Google Scholar]
  13. Glanville N., Ranki M., Morser J., Kääriäinen L., Smith A. E. 1976b; Initiation of translation directed by Semliki Forest virus 42 S and 26 S RNAs in vitro. Proceedings of the National Academy of Sciences of the United States of America 73:3059–3063
     [Google Scholar]
  14. Kääriäinen L., Gomatos P. J. 1969; A kinetic analysis of the synthesis in BHK21 cells of RNAs specific for Semliki Forest virus. Journal of General Virology 5:251–265
     [Google Scholar]
  15. Kääriäinen L., Keranen S., Lachmi B., Soderlund H., Tuomi K., Ulmanen I. 1975; Replication of Semliki Forest virus. Medical Biology 53:342–352
     [Google Scholar]
  16. Kennedy S. L. T. 1976; Sequence relationships between the genome and the intracellular RNA species of standard and defective-interfering Semliki Forest virus. Journal of Molecular Biology 108:491–511
     [Google Scholar]
  17. Keränen S., Kääriäinen L. 1974; Isolation and basic characterization of temperature-sensitive mutants from Semliki Forest virus. Acta Pathologica et Microbiologica Scandinavica B 82:810–820
     [Google Scholar]
  18. Keranen S., Kääriäinen L. 1975; Proteins synthesized by Semliki Forest virus and its 16 temperature-sensitive mutants. Journal of Virology 16:388–396
     [Google Scholar]
  19. Levin J. G., Friedman R. M. 1971; Analysis of arbovirus ribonucleic acid forms by polyacrylamide gel electrophoresis. Journal of Virology 7:504–514
     [Google Scholar]
  20. Lachmi B., Kääriäinen L. 1976; Sequential translation of nonstructural proteins in cells infected with a Semliki Forest virus mutant. Proceedings of the National Academy of Sciences of the United States of America 73:1936–1940
     [Google Scholar]
  21. Lachmi B., Kääriäinen L. 1977; Control of protein synthesis in Semliki Forest virus-infected cells. Journal of Virology 22:142–149
     [Google Scholar]
  22. Martin B. A. B., Burke D. C. 1974; The replication of Semliki Forest virus. Journal of General Virology 24:45–66
     [Google Scholar]
  23. Sawicki D. L., Gomatos P. J. 1976; Replication of Semliki Forest virus: polyadenylate in plus-strand RNA and polyuridylate in minus-strand RNA. Journal of Virology 20:446–464
     [Google Scholar]
  24. Scheele C. M., Pfefferkorn E. R. 1969; Inhibition of interjacent ribonucleic acid (26 S) synthesis in cells infected by Sindbis virus. Journal of Virology 4:117–122
     [Google Scholar]
  25. Simmons D. T., Strauss J. H. 1972a; Replication of Sindbis virus. I. Relative size and genetic content of 26 S and 49 S RNA. Journal of Molecular Biology 71:599–614
     [Google Scholar]
  26. Simmons D. T., Strauss J. H. 1972b; Replication of Sindbis virus. II. Multiple forms of double-stranded RNA isolated from infected cells. Journal of Molecular Biology 71:615–631
     [Google Scholar]
  27. Simmons D. T., Strauss J. H. 1974; Translation of Sindbis virus 26 S RNA and 49 S RNA in lysates of rabbit reticulocytes. Journal of Molecular Biology 86:397–409
     [Google Scholar]
  28. Sonnabend J. A., Martin E. M., Mecs E. 1967; Viral specific RNAs in infected cells. Nature, London 213:365–367
     [Google Scholar]
  29. Waite M. R. F. 1973; Protein synthesis directed by an RNA– temperature-sensitive mutant of Sindbis virus. Journal of Virology 11:198–206
     [Google Scholar]
  30. Wengler G., Beato M., Hackemack B. A. 1974; Translation of 26 S virus-specific RNA from Semliki Forest virus-infected cells in vitro. >Virology 61:120–128
     [Google Scholar]
  31. Wengler G., Wengler G. 1976; Localization of the 26 S RNA sequence on the viral genome type 42 S RNA isolated from SFV infected cells. Virology 73:190–199
     [Google Scholar]
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RNA Synthesis Directed by a Temperature-Sensitive Mutant of Semliki Forest Virus
J. Gen. Virol. 37, 399 (1977); https://doi.org/10.1099/0022-1317-37-2-399
/content/journal/jgv/10.1099/0022-1317-37-2-399
/content/journal/jgv/10.1099/0022-1317-37-2-399
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