Isolation and Characterization of Bacteriophages for Free

Abstract

Summary

We have isolated 38 viruses capable of infecting . Twenty-eight of these isolates were DNA phages with a prolate cylindrical head and a long flexible tail. Each of them infected only one of the cell types, the swarmer cell, and many proved to be capable of establishing a lysogenic relationship with their host. Despite structural similarity and host cell specificity, the majority of these DNA phages appeared to be genetically distinct as shown by R1 restriction patterns of their DNA and by host range on phage resistant and lysogenic strains. Two isolates were small RNA phages, structurally similar to the RNA phages, which specifically adsorbed to pili on the swarmer cell. The remaining eight isolates were DNA phages with polyhedral heads ranging from 60 to 160 nm in diameter. Six had contractile tails ranging from 50 to 160 nm in length, while two had long flexible non-contractile tails. Each of these isolates was capable of attaching to all cell types and two were capable of mediating generalized transduction between strains of (Ely & Johnson, 1977).

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/content/journal/jgv/10.1099/0022-1317-37-2-323
1977-11-01
2024-03-28
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References

  1. Agabian-Keshishian N., Shapiro L. 1970; Stalked bacteria: properties of deoxyribonucleic acid bacteriophage ϕ CbK. Journal of Virology 5:795–800
    [Google Scholar]
  2. Bendis L., Shapiro L. 1970; Properties of Caulobacter ribonucleic acid bacteriophage ϕ Cb5. Journal of Virology 6:847–854
    [Google Scholar]
  3. Bradley D. E. 1966; The fluorescent staining of bacteriophage nucleic acids. Journal ofGeneral Microbiology 44:383–391
    [Google Scholar]
  4. Driggers L. J., Schmidt J. M. 1970; Induction of defective and temperate bacteriophages in Caulobacter. Journal of General Virology 6:421–427
    [Google Scholar]
  5. Ely B., Johnson R. C. 1977; Generalized transduction in Caulobacter crescentus. Genetics 87: (in the press)
    [Google Scholar]
  6. Ely B., Weppelman R. M., Massey H. C. Jun, Hartman P. E. 1974; Some improved methods in P22 transduction. Genetics 76:625–631
    [Google Scholar]
  7. Helling R. B., Goodman H. M., Boyer H. W. 1974; Analysis of endonuclease R TcoRf fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. Journal of Virology 14:1235–1244
    [Google Scholar]
  8. Leonard K. R., Kleinschmidt A. K., Agabian N., Shapiro L., Maizel J. V. Jun 1972; Structural Studies on the capsid of Caulobacter crescentus bacteriophage ϕ CbK. Journal of Molecular Biology 71:201–216
    [Google Scholar]
  9. Newton A., Allebach E. 1975; Gene transfer in Caulobacter crescentus: polarized inheritance of genetic markers. Genetics 50:1–11
    [Google Scholar]
  10. Poindexter J. S. 1964; Biological properties and classification of the Caulobacter Group. Bacteriological Renews 28:231–295
    [Google Scholar]
  11. Potter S. S., Newbold J. E., Hutchison C. A., Edgell M. J. 1975; Specific cleavage analysis of mammalian mitochondrial DNA. Proceedings of the National Academy of Sciences of the United States of America 72:4496–4500
    [Google Scholar]
  12. Schmidt J. M., Stanier R. Y. 1965; Isolation and characterization of bacteriophages active against stalked bacteria. Journal of General Microbiology 39:95–107
    [Google Scholar]
  13. Shapiro L. 1976; Differentiation in the Caulobacter life cycle. Annual Reviews of Microbiology 30:377–407
    [Google Scholar]
  14. Szeyko G. H., Gerencser V. F. 1967; Fresh water bacteriophages for Caulobacter. Bacteriological Proceedings 1967:27–28
    [Google Scholar]
  15. Thompson R., Hughes S. G., Broda P. 1974; Plasmid identification using specific endonucleases. Molecular and General Genetics 133:141–149
    [Google Scholar]
  16. West D., Lagenaur C., Agabian N. 1976; Isolation and characterization of Caulobacter crescentus bacteriophage 0Cdl. Journal of Virology 17:568–575
    [Google Scholar]
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