1887

Abstract

Summary

The rate of virus and cell DNA synthesis was studied in human embryonic lung cells pre-treated with 5-iodo-2′-deoxyuridine (IdUrd) and exposed to cytomegalovirus (CMV) or medium. Analysis of DNA in CMV-infected cells following sequential 4 h pulses with H-thymidine indicated that a temporal relationship existed in the pattern of virus and cell DNA synthesis. The pattern of DNA replication in infected cells resembled that of a typical cell cycle, whereas the rate of cell DNA synthesis in uninfected cells remained low throughout the study. Increased rates of cell and virus DNA synthesis began concomitantly at 16 h post-infection and reached a maximum at 36 h post-infection. The rate of DNA synthesis then declined and remained at lower levels until 48 h post-infection. This was subsequently followed by a second increase in the rate of cell and virus DNA synthesis. The rates of cell and virus DNA replication were similar throughout the study in that increased and decreased rates of synthesis occurred simultaneously. It was of interest to note that CMV induced cell DNA replication in IDUrd arrested cells; in contrast, addition of fresh serum did not induce a similar increase in the rate of DNA synthesis in IdUrd arrested, but uninfected, cells.

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/content/journal/jgv/10.1099/0022-1317-37-1-65
1977-10-01
2024-03-28
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References

  1. Ben-Porat T., Kaplan A. S. 1973; Replication - biochemical aspects. In The Herpesviruses pp 163–220 Edited by S A. Kaplan, New York: Academic Press;
    [Google Scholar]
  2. Crawford L., Lee A. 1964; The nucleic acid of human cytomegalovirus. Virology 23:105–107
    [Google Scholar]
  3. Darlington R. W., Granoff A. 1973; Replication - biological aspects. In The Herpesviruses, pp93–132 Edited by Kaplan A. S. New York: Academic Press;
    [Google Scholar]
  4. Darlington R. W., Moss L. H. 1968; Herpesvirus envelope. Journal of Virology 2:48–55
    [Google Scholar]
  5. Demarchi J. M., Kaplan A. S. 1976; Replication of human cytomegalovirus DNA: lack of dependence on cell DNA synthesis. Journal of Virology 18:1063–1070
    [Google Scholar]
  6. Fried M., Pitts H. D. 1968; Replication of polyoma virus DNA. I. A resting cell system for biochemical studies on polyoma virus. Virology 34:761–770
    [Google Scholar]
  7. Furukawa T., Fioretti A., Plotkin S. 1973; Growth characteristics of cytomegalovirus in human fibroblasts with demonstration of protein synthesis early in viral replication. Journal of Virology991–997
    [Google Scholar]
  8. Furukawa T., Tanaka S., Plotkin S. A. 1975a; Stimulation of macromolecular synthesis in guinea pig cells by human CMV. Proceedings of the Society for Experimental Biology and Medicine 148:211–214
    [Google Scholar]
  9. Furukawa T., Tanaka S., Plotkin S. A. 1975b; Restricted growth of human cytomegalovirus in TJV-irradiated WI-38 human fibroblasts. Proceedings of the Society for Experimental Biology and Medicine 148:1249–1251
    [Google Scholar]
  10. Henry P., Black P. H., Oxman M. N., Weissman S. M. 1966; Stimulation of DNA synthesis in mouse cell line 3T3 by simian virus 40. Proceedings of the National Academy of Sciences of the United States of America 56:1170–1176
    [Google Scholar]
  11. Holmes A. W., Gilson J., Deinhardt F. 1964; Inhibition of interferon production by 5-iodo-2-deoxy-uridine. Virology 24:229–232
    [Google Scholar]
  12. Huang E., Chen S., Pagano J. S. 1973; Human cytomegalovirus. I. Purification and characterization of viral DNA. Journal of Virology 12:1473–1481
    [Google Scholar]
  13. Huang E. 1975; Human cytomegalovirus III virus induced DNA polymerase. Journal of Virology 16:298–310
    [Google Scholar]
  14. Plummer G., Goodheart C. R., Henson D., Bowling C. P. 1969; A comparative study of the DNA density and behaviour in tissue culture of fourteen different herpesviruses. Virology 39:134–137
    [Google Scholar]
  15. Shackney S. E., Ford S. S., Wittig A. B. 1973; The effects of counting threshold and emulsion exposure duration on the percent labeled mitosis curve and their implications for cell cycle analysis. Cancer Research 33:2726–2731
    [Google Scholar]
  16. Stockdale H. F., Okazaki K., Nameroff M., Holtzer H. 1964; 5-Bromodeoxyuridine: effect of myogenesis in vitro. Science 146:533–535
    [Google Scholar]
  17. St Jeor S., Rapp F. 1973a; Cytomegalovirus replication in cells pretreated with 5′-iodo-2′-deoxyuridine. Journal of Virology 11:986–990
    [Google Scholar]
  18. St Jeor S., Rapp F. 1973b; Cytomegalovirus: conversion of non-permissive cells to permissive state for virus replication. Science 181:1060–1061
    [Google Scholar]
  19. St Jeor S. C., Albrecht T. B., Funk F. D., Rapp F. 1974; Stimulation of cellular DNA synthesis by human cytomegalovirus. Journal of Virology 13:353–362
    [Google Scholar]
  20. St Jeor S. C., Hutt R., Rapp F. 1975; Relationship between human cytomegalovirus and cell DNA synthesis. Abstracts of the Annual Meeting of the American Society for Microbiology p 239
    [Google Scholar]
  21. Wentworth B. B., French L. 1970; Plaque assay of cytomegalovirus strains of human origin. Proceedings of the Society for Experimental Biology and Medicine 135:253–258
    [Google Scholar]
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