1887

Abstract

Summary

Cells infected with respiratory syncytial (RS) virus eventually die but there appears to be no specific mechanism for shutting off cellular synthesis of macromolecules. DNA and RNA synthesis, as measured by the incorporation of labelled thymidine or uridine, do not begin to shut down until some time between 11 and 18 h after infection. By 18 h their rates of synthesis are reduced to approx. 50% for DNA and 35% for RNA.

Protein synthesis continues throughout the course of infection at approximately the same rate. Synthesis of most of the cellular polypeptides also continues, but the distribution of polypeptides of high and low mol. wt. shifts. The increase in the proportion of those of high mol. wt. includes a peak that represents one of the seven previously identified virion polypeptides.

Another consequence of RS virus infection is an increase in glucosamine incorporation, beginning near the end of the virus eclipse period (12 h after infection), which may be associated with virion glycoprotein synthesis. Polyacrylamide-gel electrophoresis of glucosamine-labelled cells reveals that at 18 h after infection two of the three previously identified virion glycoproteins are present.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-37-1-53
1977-10-01
2024-04-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/37/1/JV0370010053.html?itemId=/content/journal/jgv/10.1099/0022-1317-37-1-53&mimeType=html&fmt=ahah

References

  1. Baldridge P., Senterfit L. B. 1976; Persistent infection of cells in culture by respiratory syncytial virus. Proceedings of the Society for Experimental Biology and Medicine 151:684–688
    [Google Scholar]
  2. Berthiaume L., Joncas J., Pavilanis V. 1964; Comparative structure, morphogenesis and biological characteristics of respiratory syncytial (RS) virus and pneumonia virus of mice (PVM). Archiv für die gesamte Virusforschung 45:39–51
    [Google Scholar]
  3. Choppin P. W., Compans R. W. 1975; Reproduction of paramyxoviruses. In Comprehensive Virology vol 4 pp 95–178 Edited by Fraenkel-Conrat H., Wagner. R. R. New York and London: Plenum Press;
    [Google Scholar]
  4. Compans R. W., Holmes K. V., Dales S., Choppin P. W. 1966; An electron microscopic study of moderate and virulent virus-cell interactions of the parainfluenza virus SV5. Virology 30:411–426
    [Google Scholar]
  5. Hodes D., Schauf V., Chanock R. M. 1974; Isolation of RNA species from HeLa cells infected with respiratory syncytial virus. Proceedings of the Society for Experimental Biology and Medicine 146:287–290
    [Google Scholar]
  6. Holmes K. V., Choppin P. W. 1966; On the role of the response of the cell membrane in determining virus virulence. Contrasting effects of the parainfluenza virus SV5 in two cell types. Journal of Experimental Medicine 124:501–520
    [Google Scholar]
  7. Levine S. 1977; Polypeptides of respiratory syncytial virus. Journal of Virology 21:427–431
    [Google Scholar]
  8. Levine S., Buthala D., Hamilton R. 1971; Late stage synchronization of respiratory syncytial virus replication. Virology 45:390–400
    [Google Scholar]
  9. Levine S., Hamilton R. 1969; Kinetics of the respiratory syncytial virus growth cycle in the HeLa cell. Archiv fur die gesamte Virusforschung 28:122–132
    [Google Scholar]
  10. Lowry O. H., Rosebrough N. H., Farr A. L., Randall R. J. 1951; Protein measurements with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  11. Norrby E., Marusyk H., Orvell C. 1970; Morphogenesis of respiratory syncytial virus in a green monkey kidney cell line (Vero). Journal of Virology 6:237–242
    [Google Scholar]
  12. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  13. Wunner W. H., Faulkner G. P., Pringle C. R. 1975; Respiratory syncytial virus: some biological and biochemical properties. In Negative Strand Viruses pp 193–201 Edited by Mahy B. W. J., Barry. R. D. New York: Academic Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-37-1-53
Loading
/content/journal/jgv/10.1099/0022-1317-37-1-53
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error