RT Journal Article SR Electronic(1) A1 Khosaka, ToshiyukiYR 1977 T1 Physicochemical Properties of a Virulent Lactobacillus Phage Containing DNA with Cohesive Ends JF Journal of General Virology, VO 37 IS 1 SP 209 OP 214 DO https://doi.org/10.1099/0022-1317-37-1-209 PB Microbiology Society, SN 1465-2099, AB Summary Physicochemical properties of a virulent Lactobacillus phage, J1, and its DNA were studied. Phage J1 was shown to consist of a hexagonal head 55 nm in diameter and a flexible, non-contractile tail 290 nm in length and 10 nm in width. The buoyant density of the phage in CsCl was measured as 1.49 g/ml. It was found that J1 DNA was a linear duplex molecule with cohesive ends and had a contour length of 12.2 µm. The single strands were intact for the length of the molecule. The melting temperature, Tm, and the buoyant density of J1 DNA correspond to a G + C content of 48% and 45%, respectively., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-37-1-209