1887

Abstract

Summary

Physicochemical properties of a virulent phage, J1, and its DNA were studied. Phage J1 was shown to consist of a hexagonal head 55 nm in diameter and a flexible, non-contractile tail 290 nm in length and 10 nm in width. The buoyant density of the phage in CsCl was measured as 1.49 g/ml. It was found that J1 DNA was a linear duplex molecule with cohesive ends and had a contour length of 12.2 µm. The single strands were intact for the length of the molecule. The melting temperature, T, and the buoyant density of J1 DNA correspond to a G + C content of 48% and 45%, respectively.

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1977-10-01
2022-01-28
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References

  1. Adams M. H. 1959 In Bacteriophage pp 454–456 New York: Academic Press;
  2. Bode V. C., Kaiser A. D. 1965; Changes in the structure and activity of A DNA in a superinfected immune bacterium. Journal of Molecular Biology 14:399–417
    [Google Scholar]
  3. Hershey A. D., Burgi E., Ingraham L. 1963; Cohesion of DNA molecules isolated from phage lambda. Proceedings of the National Academy of Sciences of the United States of America 49:748–755
    [Google Scholar]
  4. Hino M., Ikebe N. 1965; Studies on the lactic acid bacteria employed for beverage production. Part II. Isolation and some properties of a bacteriophage infected during the fermentation of lactic acid beverage. Journal of the Agricultural Chemical Society of Japan 39:472–476
    [Google Scholar]
  5. Ichioka S., Ishiwa H. 1973; Genetic studies on temperature-sensitive mutants of; Lactobacillus casei phage J1. Virus (Japan) 23:30–38
    [Google Scholar]
  6. Kleinschmidt A. K. 1968; Monolayer techniques in electron microscopy of nucleic acid molecules. In Methods in Enzymology vol XII part B pp 361–377 Edited by Crossman L., Moldave. K. New York and London: Academic Press;
    [Google Scholar]
  7. Lee C. S., Davidson N. 1970; Studies on the deoxyribonucleic acids from bacteriophages of Micrococcus lysodeikticus. Virology 40:102–117
    [Google Scholar]
  8. Lee C. S., Davidson N., Scaletti J. V. 1968; Covalently closed circular DNA from M. lysodeikticus cells infected with phage N1. Biochemical and Biophysical Research Communications 32:752–756
    [Google Scholar]
  9. Machattie L. A., Thomas C. A. Jun 1964; DNA from bacteriophage lambda: molecular length and conformation. Science 144:1142–1144
    [Google Scholar]
  10. Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  11. Murata A., Soeda E., Saruno R. 1969; Factors influencing plaque formation by bacteriophages of Lactobacillus acidophilus. Journal of the Agricultural Chemical Society of Japan 39:472–476
    [Google Scholar]
  12. Sakurai T., Takahashi T., Kamiyama K., Arai H. 1969; Isolation of bacteriophages parasitic on Lactobacillus casei and L. plantarum and their several properties. Virus (Japan) 19:311–324
    [Google Scholar]
  13. Schildkraut C. L., Marmur J., Doty P. 1962; Determination of the base composition of deoxyribonucleic acid rom its buoyant density in CsCl. Journal of Molecular Biology 4:430–443
    [Google Scholar]
  14. Wang J. C., Davidson N. 1966a; Thermodynamic and kinetic studies on the interconversion between the linear and circular forms of phage A DNA. Journal of Molecular Biology 15:111–123
    [Google Scholar]
  15. Wang J. C., Davidson N. 1966b; On the probability of ring closure of A DNA. Journal of Molecular Biology 19:469–482
    [Google Scholar]
  16. Watanabe K., Takesue S. 1972; The requirement for calcium in infection with Lactobacillus phage. Journal of General Virology 17:19–30
    [Google Scholar]
  17. Watanabe K., Takesue S. 1975; Use of L-rhamnose to study irreversible adsorption of bacteriophage PL-1 to a strain of Lactobacillus casei. Journal of General Virology 28:29–35
    [Google Scholar]
  18. Watanabe K., Takesue S., Jin-Nai K., Yoshikawa T. 1970; Bacteriophage active against the lactic acid beverage-producing bacterium, Lactobacillus casei. Applied Microbiology 20:409–415
    [Google Scholar]
  19. Weissbach A., Lipton A., Lisio A. 1966; Intracellular forms of A deoxyribonucleic acid in Escherichia coli infected with clear or virulent mutants of bacteriophage A. Journal of Bacteriology 91:1489–1493
    [Google Scholar]
  20. Wetmur J. G., Davidson N., Scaletti J. V. 1966; Properties of DNA of bacteriophage N1, a DNA with reversible circularity. Biochemical and Biophysical Research Communications 25:684–688
    [Google Scholar]
  21. Yamamoto K. R., Alberts B. M., Benzinger R., Lawhorne L., Treiber G. 1970; Rapid bacteriophage sedimentation in the presence of polyethylene glycol and its application to large scale virus purification. Virology 40:734–744
    [Google Scholar]
  22. Yokokura T. 1971; Phage receptor material in Lactobacillus casei cell wall. I. Effect of L-rhamnose on phage adsorption to the cell wall. Japanese Journal of Microbiology 15:457–463
    [Google Scholar]
  23. Yokokura T. 1977; Phage receptor material in Lactobacillus casei. Journal of General Microbiology 100:139–145
    [Google Scholar]
  24. Young E. T. II, Sinsheimer R. L. 1964; Novel intracellular forms of lambda DNA. Journal of Molecular Biology 10:562–564
    [Google Scholar]
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