1887

Abstract

Summary

Kethoxal bis (thiosemicarbazone) (KTS) inhibited replication of, and plaque formation by, vesicular stomatitis virus (VSV) in chick embryo cells. No other thiosemicarbazones tested were effective. Virus-specific m-RNA and protein synthesis were inhibited by KTS. However, virion RNA-dependent RNA synthesis was not inhibited by the drug. Treatment of VSV virions directly with KTS produced enhancement, rather than inactivation, of plaque formation.

KTS inhibited cellular DNA and RNA synthesis by 67 and 25% respectively. Since cellular DNA and RNA synthesis are not required for VSV replication, the inhibition of these processes is probably unrelated to the antiviral activity of KTS. Cellular protein synthesis was inhibited 24% by KTS. Unexpectedly, synthesis of four proteins was induced in KTS-treated uninfected cells.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-37-1-183
1977-10-01
2022-07-07
Loading full text...

Full text loading...

/deliver/fulltext/jgv/37/1/JV0370010183.html?itemId=/content/journal/jgv/10.1099/0022-1317-37-1-183&mimeType=html&fmt=ahah

References

  1. Bauer D. J., sadler P. W. 1960; The structure-activity relationships of the antiviral chemotherapeutic activity of isatin β-thiosemicarbazones. British Journal of Pharmacology 15:101–110
    [Google Scholar]
  2. Bauer D. I., Apostolov K., Selway J. W. T. 1970; Activity of methisazone against RNA viruses. Annals of the New York Academy of Sciences 173:314–319
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Booth B. A., Sartorelli A. C. 1967; Metabolic effects of copper in intact cells: comparative activity of cupric chloride and the cupric chelate of kethoxal bis (thiosemicarbazone). Molecular Pharmacology 3:290–302
    [Google Scholar]
  5. Booth B. A., Moore E. C., Sartorelli A. C. 1971; Metabolic effects of some tumor-inhibitory pyridine carboxaldehyde thiosemicarbazones. Cancer Research 31:228–234
    [Google Scholar]
  6. Campbell J. B., Maes R. F., Wiktor T. J., Koprowski H. 1968; The inhibition of rabies virus by arabinosy cytosine. Studies on the mechanism and specificity of action. Virology 34:701–708
    [Google Scholar]
  7. Crim J. A., Petering H. G. 1967; The antitumor activity of Cu (Ⅱ) KTS, the copper (Ⅱ) chelate of 3-ethoxy-2-oxobutyraldehyde bis (thiosemicarbazone). Cancer Research 27:1278–1285
    [Google Scholar]
  8. Flamand A., Eishop D. H. L. 1974; In vivo synthesis of RNA by vesicular stomatitis virus and its mutants. Journal of Molecular Biology 87:31–53
    [Google Scholar]
  9. Follett E. A. C., Pringle C. R., Winner W. H., Skehel J. J. 1974; Virus replication in enucleate cells: vesicular stomatitis virus and influenza virus. Journal of Virology 13:394–399
    [Google Scholar]
  10. Haase A. T., Levinson W. 1973; Inhibition of RNA slow viruses by thiosemicarbazones. Biochemical and Biophysical Research Communications 51:875–880
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Levinson W. 1967; Fragmentation of the nucleus in Rous sarcoma virus infected chick embryo cells. Virology 32:74–83
    [Google Scholar]
  13. Levinson W. 1973; Inhibition of viruses, tumors and pathogenic micro-organisms by isatin β-thiosemi-carbazone and other thiosemicarbazones. In Selective Inhibitors of Viral Function pp 213–226 Edited by Carter W. Cleveland: CRC Press;
    [Google Scholar]
  14. Levinson W., Helling R. 1976; Inactivation of lambda phage infectivity and lambda deoxyribonucleic acid transfection by TV-methyl isatin betathiosemicarbazone. Antimicrobial Agents and Chemotherapy 9:160–163
    [Google Scholar]
  15. Levinson W., Coleman V., Woodson B., Rabson A., Lanier J., Whitcher J., Dawson C. 1974; Inactivation of herpes simplex virus by thiosemicarbazone and certain cations. Antimicrobial Agents and Chemotherapy 5:398–402
    [Google Scholar]
  16. Levinson W., Faras A., Morris R., Mikelens P., Ringold G., Kass S., Levinson B., Jackson J. 1973a; Effect of N-methyl isatin betathiosemicarbazone on Rous sarcoma virus, several isolated enzymes and other viruses. In ICN-UCLA Symposium on Virus Research pp 403–409 Edited by Fox F., Robinson W. New York: Academic Press;
    [Google Scholar]
  17. Levinson W., Faras A., Woodson B., Jackson J., Bishop J. 1973b; Inhibition of RNA dependent DNA polymerase of Rous sarcoma virus by thiosemicarbazones and several cations. Proceedings of the National Academy of Sciences of the United States of America 70:164–168
    [Google Scholar]
  18. Levinson W., Woodson B., Jackson J. 1971; Inactivation of Rous sarcoma virus on contact with TV-substituted isatin beta-thiosemicarbazone. Nature New Biology 232:116–118
    [Google Scholar]
  19. Levy J., Levy, S., Levinson W. 1976; Inactivation of murine RNA tumor viruses by isatin betathiosemicarbazone, its derivative and analogs. Virology 74:426–431
    [Google Scholar]
  20. Loening U. R. 1969; Determination of the molecular weight of RNA by polyacrylamide gel electrophoresis. Biochemical Journal 113:131–138
    [Google Scholar]
  21. Logan J. C., Fox M. P., Morgan J. H., Makohon A. M., Pfau C. J. 1975; Arena virus inactivation on contact with N-substituted isatin beta-thiosemicarbazone and certain cations. Journal of General Virology 28:271–284
    [Google Scholar]
  22. Mikelens P., Woodson B., Levinson W. 1976; Association of nucleic acids with complexes of N-methyl isatin beta-thiosemicarbazone and copper. Biochemical Pharmacology 26:821–827
    [Google Scholar]
  23. Pearson G. D., Zimmerman E. F. 1969; Inhibition of poliovirus replication by TV-methyl isatin B-4′-4′ dibutyl thiosemicarbazone. Virology 38:641–650
    [Google Scholar]
  24. Repik P., Flamand A., Bishop D. H. L. 1974; Effect of interferon upon the primary and secondary transcription of vesicular stomatitis and influenza viruses. Journal of Virology 14:1169–1178
    [Google Scholar]
  25. Schincariol A. L., Howatson A. F. 1970; Replication of vesicular stomatitis virus. Virology 42:732–743
    [Google Scholar]
  26. Studier F. W. 1973; Analysis of bacteriophage T7 early RNA’s and proteins on slab gels. Journal of Molecular Biology 79:237–248
    [Google Scholar]
  27. Turner W., Bauer D., Nimmo-Smith R. H. 1962; Eczema vaccinatum treated with TV-methyl isatin β-thiosemicarbazone. British Medical Journal i:1317–1319
    [Google Scholar]
  28. van Giessen G. T., Petering H. G. 1968; Metal complexes of 3-ethoxy-2-oxo-butyraldehyde bis (thiosemicarbazone) and related ligands as antitumor agents. Journal of Medicinal Chemistry 11695–699
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-37-1-183
Loading
/content/journal/jgv/10.1099/0022-1317-37-1-183
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error