1887

Abstract

SUMMARY

Methods are described for the preparation of S-methionine-labelled influenza viruses, the purification of the nucleoprotein (NP) and matrix (M) proteins and the separation of peptides obtained by protease digestion by two-dimensional thin-layer chromatography. The maps of the M proteins of A/Okuda/57 (HN) and A/Finland/4/74 (HN) were very similar overall but differed in three peptides. Hence they could be clearly distinguished. Maps of the NP proteins of the same strains showed a greater number of differences.

A recombinant strain having the haemagglutinin and neuraminidase of the A/Finland/4/74 parent and the virulence of the A/Okuda/57 parent was shown to have the M and P proteins of A/Okuda/57.

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1977-09-01
2024-04-18
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References

  1. Bernkopf H. 1949; Cultivation of influenza virus in the chorio-allantoic membrane of de-embryonated eggs. Proceedings of the Society for Experimental Biology and Medicine 72:680–682
    [Google Scholar]
  2. Brand C. M., Stealey V. M., Rowe J. 1977; Structural differences in matrix proteins of human influenza A strains as revealed by I2rT-labelled peptide maps. Journal of General Virology 36: (in the press)
    [Google Scholar]
  3. Bray D., Brownlee S. M. 1973; Peptide mapping of proteins from acrylamide gels. Analytical Biochemistry 55:213–221
    [Google Scholar]
  4. Choppin P. W., Compans R. W. 1975; The structure of influenza virus. In The Influenza Viruses and Influenza p 15–51 Edited by Kilbourne E. D. London: Academic Press;
    [Google Scholar]
  5. Clegg J. C. S., Brzeski H., Kennedy S. I. T. 1976; RNA polymerase components in Semliki Forest virus-infected cells: synthesis from large precursors. Journal of General Virology 32:413–430
    [Google Scholar]
  6. Fazekas de ST Groth S., White D. O. 1958; An improved assay for the infectivity of influenza viruses. Journal of Hygiene 56:151–162
    [Google Scholar]
  7. Hayman M. J., Skehel J. J., Crumpton M. J. 1973; Purification of virus glycoproteins by affinity chromatography using Lens culinaris phytohaemagglutinin. FEES Letters 29:185–188
    [Google Scholar]
  8. Inglis S. C., Carroll A. R., Lamb R. A., Mahy B. W. J. 1976; Polypeptides specified by the influenza virus genome. Virology 74:489–503
    [Google Scholar]
  9. Kelly D. C., Dimmock N. J. 1974; Fowl plague virus replication in mammalian cell-avian erythrocyte heterokaryons: studies concerning the actinomycin D and ultraviolet light sensitive phase in influenza virus replication. Virology 61:210–222
    [Google Scholar]
  10. Kilbourne E. D., Choppin P. W., Schulze I. T., Scholtissek C., Bucher D. L. 1972; Influenza virus polypeptides and antigens – summary of influenza workshop I. Journal of Infectious Diseases 125:447–455
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Lamb R. A., Choppin P. W. 1976; Synthesis of influenza virus proteins in infected cells: translation of viral polypeptides including three P polypeptides, from RNA produced by primary transcription. Virology 74:504–519
    [Google Scholar]
  13. Laver W. G., Downie J. C. 1976; Influenza virus recombination. 1. Matrix protein markers and segregation during mixed infections. Virology 70:105–117
    [Google Scholar]
  14. Lief F. S., Fabiyi A., Henle W. 1958; Antigenic analyses of influenza viruses by complement fixation.I. The production of antibodies to the soluble antigen in guinea pigs. Journal of Immunology 80:53–65
    [Google Scholar]
  15. McCahon D., Beare A. S., Stealey V. 1976; The production of live attenuated influenza A strains by recombination with A/Okuda/57 (H1N2). Postgraduate Medical Journal 52:389–394
    [Google Scholar]
  16. Oxford J. S., Schild G. C. 1975; Immunological studies with influenza virus matrix protein. In Negative Strand Viruses p 611–620 Edited by Mahy B. W. J., Barry R. D. London: Academic Press;
    [Google Scholar]
  17. Palese P. 1977; The genes of influenza virus. Cell 10:1–10
    [Google Scholar]
  18. Schild G. C. 1972; Evidence for a new type-specific structural antigen of the influenza virus particle. Journal of General Virology 15:99–103
    [Google Scholar]
  19. Studier R. W. 1973; Analysis of bacteriophage T7 early RNAs and proteins on slab gel. Journal of Molecular Biology 79:237–248
    [Google Scholar]
  20. Webster R. G., Laver W. G. 1967; Preparation and properties of antibody directed specifically against the neuraminidase of influenza virus. Journal of Immunology 99:49–55
    [Google Scholar]
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