In an attempt to define the molecular events involved in induction of interferon, various parameters of chick cells infected with human adenovirus type 5 were analysed. It was shown by digestion with various proteolytic enzymes and by disruption of the purified virus that induction of interferon requires the interaction of infectious virus with the chick cells. Analysis of adenovirus-infected chick cells by immunological and biochemical techniques indicated that most of the cells produce some virus-specific components, and that temperature sensitive mutants which fail to induce interferon at the restrictive temperature fail to synthesize late components at that temperature. However, since it has been shown that interferon can be induced in the absence of DNA synthesis, these studies conclude that interferon induction results from an early interaction between virus (or virus product) and chick cells and moreover that this interaction is also necessary for the synthesis of virus DNA in this system.
AtkinsG. J.,
LancashireC. L.1976; The induction of interferon by temperature sensitive mutants of Sindbis virus: its relationship to double stranded RNA synthesis and cytopathic effect. Journal of General Virology 30:157–165
BakayM.,
BurkeD. C.1972; The production of interferon in chick cells infected with DNA viruses: a search for double-stranded RNA. Journal of General Virology 16:399–403
BeladiL.,
PusztaiR.1967; Interferon-like substance produced in chick fibroblast cells inoculated with human adenoviruses. Zeitschrift fur Naturforschung 226:165–169
BurkeD. C.,
RussellW. C.1975; Control of nucleic acid replication in cells infected with animal viruses. In SGM Symposium on Control Processes in Virus Multiplication no 25: p 253–293 London: Cambridge University Press;
LaiM. H. T.,
JoklikW. K.1973; The induction of interferon by temperature-sensitive mutants of reovirus, UV-irradiated reovirus, and the subviral reovirus particles. Virology 51:191–204
LampsonG. P.,
TytellA. A.,
FieldA. K,
NemesM. M. Q.,
HillemanM. R.1967; Inducers of interferon and host resistance. I. Double-stranded RNA from extracts of Penicillium funiculosum. Proceedings of the National Academy of Sciences of the United States of America 58:782–789
LockartR. Z.,
BaylissN. L.,
ToyS. T.,
YinF. H.1968; Viral events necessary for the induction of interferon in chick embryo cells. Journal of Virology 2:962–965
LowryO. H.,
RosebroughN. J.,
FarrA. L.,
RandallR. J.1951; Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193:265–275
LucasJ. J.,
GinsbergH. S.1972; Identification of double stranded virus specific ribonucleic acid in KB cells infected with type 2 adenovirus. Biochemical and Biophysical Research Communications 49:39–44
MaizelJ. V.Jun,
WhiteD. O.,
ScharffM. D.1968; The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and comparison of type 2, jA and 12. Virology 36:115–125
PrageL.,
PetterssonU.,
HoglundS.,
Longerg-HolmK.,
PhilipsonL.1970; Structural proteins of adenovirus. IV. Sequential degradation of the adenovirus type 2 virion. Virology 42:341–358
PusztaiR.,
BeladiI.,
BakayM.,
MucsiI.1969; Effect of ultraviolet irradiation and heating on the interferon-inducing capacity of human adenoviruses. Journal of General Virology 4:169–176
RussellW. C.,
HayashiK.,
SandersonP. J.,
PereiraH. G.1967; Adenovirus antigens-a study of their properties and sequential development in infection. Journal of General Virology 1:495–508
RussellW. C.,
NewmanC.,
WilliamsJ. F.1972; Characterisation of temperature sensitive mutants of adenovirus type 5-serology. Journal of General Virology 17:265–279
UstacelebiS.,
WilliamsJ. F.1972; Temperature sensitive mutants of adenovirus defective in interferon induction at non-permissive temperature. Nature, London 235:52–53
WilliamsJ. F.,
GharpureM.,
UstacelebiS.,
McDonaldS.1971; Isolation of temperature sensitive mutants of adenovirus type 5. Journal of General Virology 11:95–101