1887

Abstract

SUMMARY

Periodate or nitrous acid treatment greatly decreases the ability of unfractionated transfer RNA (tRNA) to be aminoacylated by tRNA-synthetases but these treatments do not affect their antiviral activity against encephalomyocarditis virus infection of mice. Bisulphite treatment of tRNA reduces its ability to be aminoacylated by 20% and has no effect on antiviral activity. Bromine water treatment of tRNA under conditions causing extensive base modifications eliminates aminoacylation and the antiviral activity of tRNA. Periodate treatment of yeast tRNA does not affect its antiviral activity and nitrous acid treatment increases its antiviral activity to that of tRNA. The ability to be aminoacylated does not therefore appear to be essential for antiviral activity of tRNA but extensive modification (bromine water treatment) does destroy antiviral activity.

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1977-08-01
2022-10-04
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References

  1. Brammer K. W. 1963; Chemical modification of viral ribonucleic acid. II. Bromination and iodination. Biochimica et Biophysica Acta 72:217–229
    [Google Scholar]
  2. Carbon J. 1971; Chemical modifications of transfer RNA. In Methods in Enzymology vol 20 pp 129–135 Edited by Moldave K., Grossman L. New York and London: Academic Press;
    [Google Scholar]
  3. Chambers R. W., Aoyagi S., Furukawa Y., Zawadzka H., Bhanot O. S. 1973; Inactivation of valine acceptor activity by a C→ U missense change in the anti–codon of yeast valine transfer ribonucleic acid. Journal of Biological Chemistry 248:5549–5551
    [Google Scholar]
  4. Dulbecco R., Smith J. D. 1960; The detection of terminal nucleoside residues in polyribonucleotides. Biochemica et Biophysica Acta 39:358–361
    [Google Scholar]
  5. Eaton M. A. W., Hutchinson D. W. 1973; Poly(5–hydroxycytidylic acid). Biochimica et Biophysica Acta 319:281–287
    [Google Scholar]
  6. Gierer A., Mundry K. W. 1958; Production of mutants of TMV by chemical alteration of its ribonucleic acid in vitro. Nature, London 182:1457
    [Google Scholar]
  7. Goddard J. P., Schulman L. H. 1972; Conversion of exposed cytidine residues to uridine residues in Escherichia coli formylmethionine transfer ribonucleic acid. Journal of Biological Chemistry 247:3864–3867
    [Google Scholar]
  8. Kucan Z., Freude K. A., Kucan I., Chambers R. W. 1971; Aminoacylation of bisulphite–modified yeast tyrosine transfer RNA. Nature New Biology 232:177–179
    [Google Scholar]
  9. Lindley I. J. D., Stebbing N. 1976; Aminoacylation of encephalomyocarditis virus RNA. Journal of General Virology 34:177–181
    [Google Scholar]
  10. Loening U. E. 1969; The determination of the molecular weight of ribonucleic acid by polyacrylamidegel– electrophoresis. Biochemical Journal 113:131–138
    [Google Scholar]
  11. Means G. E., Fraenkel-Conrat H. 1971; Effects of bromine on the template and messenger specificities of polynucleotides. Biochimica et Biophysica Acta 247:441–448
    [Google Scholar]
  12. Peto R., Pike M. C. 1973; Conservatism of the approximation ∑(O-E)2/E in the logrank test for survival data or tumour incidence data. Biometrics 29:579–584
    [Google Scholar]
  13. Stebbing N., Grantham C. A., Carey N. H. 1976a; Antiviral activity of single–stranded homopolynucleotides against encephalomyocarditis virus and Semliki Forest virus in adult mice without interferon induction. Journal of General Virology 30:21–39
    [Google Scholar]
  14. Stebbing N., Grantham C. A., Kaminski F., Lindley I. J. D. 1976b; Protection of mice against encephalomyocarditis virus infection by preparations of transfer RNA. Journal of General Virology 34:73–85
    [Google Scholar]
  15. Stebbing N., Lindley I. J. D., Eaton M. A. W. 1977; The direct antiviral activity of single–stranded polyribonucleotides. I. Potentiation of activity by mixtures of polymers which do not anneal. Proceedings of the Royal Society (in the press)
    [Google Scholar]
  16. Zamecnik P. C., Yu C. T. 1971; Modification of tRNA’s by bromination. In Methods in Enzymology vol 20 pp 175–178 Edited by Moldave K., Grossman L. New York & London: Academic Press;
    [Google Scholar]
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