Reagents which Inhibit Disulphide Bond Formation Stabilize Human Fibroblast Interferon Free

Abstract

SUMMARY

Fibroblast interferon may be stabilized against many inactivating influences by the addition of certain simple sulphydryl reagents. The use of such easily removable and relatively non-toxic stabilizers should help in the preparation and purification of fibroblast interferon for clinical use.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-36-2-323
1977-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/36/2/JV0360020323.html?itemId=/content/journal/jgv/10.1099/0022-1317-36-2-323&mimeType=html&fmt=ahah

References

  1. Davidson B. E., Hird F. J. R. 1967; The reactivity of the disulphide bonds of purified proteins in relationship to primary structure. Biochemical Journal 104:473–479
    [Google Scholar]
  2. De Somer P., Joniau M., Edy V. G., Billiau A. 1973; Mass production of human interferon in diploid cells. Proceedings of the Tissue Culture Association Workshop Lake Placid May 1973 39–46
    [Google Scholar]
  3. Edy V. G., Billiau A., Joniau M., De Somer P. 1974; Stabilisation of human and mouse interferons towards shaking and guanidine hydrochloride by acid pH. Proceedings of the Society for Experimental Biology and Medicine 146:249–253
    [Google Scholar]
  4. Fantes K. H., O’Neill C. 1964; Some similarities between a viral inhibitor of plant origin and chick interferon. Nature, London 203:1048–1050
    [Google Scholar]
  5. Merigan T. C., Winget C. A., Dixon G. M. 1965; Purification and characterisation of vertebrate interferons. Journal of Molecular Biology 13:679–691
    [Google Scholar]
  6. Schonne E. 1966; Properties of rat tumour interferon. Biochimica et Biophysica Acta 15:429–439
    [Google Scholar]
  7. Tanford C. 1968; Protein denaturation. Advances in Protein Chemistry 23:121–282
    [Google Scholar]
  8. Warner R. C., Levy M. 1958; Denaturation of bovine serum albumin. Journal of the American Chemical Society 80:5735–5744
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-36-2-323
Loading
/content/journal/jgv/10.1099/0022-1317-36-2-323
Loading

Data & Media loading...

Most cited Most Cited RSS feed