1887

Abstract

SUMMARY

Five temperature-sensitive mutants (ts 1 to 5) were isolated from a stock of the Moloney strain of murine sarcoma leukaemia virus complex which had been mutagenized by ultraviolet irradiation or -methyl-′-nitro--nitrosoguanidine. In mouse cells at the non-permissive temperature the mutants formed fewer foci of transformed cells than at the permissive temperature.

The ts mutants were characterized by testing: (1) murine leukaemia virus (MuLV) clones from the ts complex, (2) the effect of additional wild type MuLV on focus formation, (3) focus formation in rat cells and (4) focus formation with pseudotypes rescued from non-producer cells. Two mutants (ts 1 and ts 3) were found to be ts MuLVs which did not possess heat labile virion proteins and were not ts in post-penetration helper functions necessary for the fixation of sarcoma virus transformation. The remaining three mutants (ts 2, ts 4 and ts 5) were ts murine sarcoma viruses which, however, showed no temperature-sensitive effect on the maintenance of transformed cell morphology nor on colony forming efficiency in soft agar.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-36-2-257
1977-08-01
2022-01-17
Loading full text...

Full text loading...

/deliver/fulltext/jgv/36/2/JV0360020257.html?itemId=/content/journal/jgv/10.1099/0022-1317-36-2-257&mimeType=html&fmt=ahah

References

  1. Bassin R. H., Tuttle N., Fischinger P. J. 1970; Isolation of murine sarcoma virus-transformed mouse cells which are negative for leukemia virus from agar suspension cultures. International Journal of Cancer 6:95–107
    [Google Scholar]
  2. Bookout J. B., Sigel M. M. 1975; Characterization of a conditional mutant of Rous sarcoma virus with alterations in early and late functions of cell transformation. Virology 67:474–486
    [Google Scholar]
  3. Duc-Nguyen H., Rosenblum E. N., Zeigel R. F. 1966; Persistent infection of a rat kidney cell line with Rauscher murine leukemia virus. Journal of Bacteriology 92:1133–1140
    [Google Scholar]
  4. Friis R. R., Toyoshima K., Vogt P. K. 1971; Conditional lethal mutants of avian sarcoma viruses. I. Physiology of ts 75 and ts 149. Virology 43:375–389
    [Google Scholar]
  5. Hunter E., Vogt P. K. 1976; Temperature-sensitive mutants of avian sarcoma viruses. Genetic recombination with wild type sarcoma virus and physiological analysis of multiple mutants. Virology 69:23–34
    [Google Scholar]
  6. Ida N., Ikawa Y., Ogawa K., Takada M., Sugano H. 1974; Cell culture from a rat brain tumor induced by intracerebral inoculation with murine sarcoma virus. Journal of the National Cancer Institute 53:431–448
    [Google Scholar]
  7. Levy J. A. 1971; Demonstration of differences in murine sarcoma virus foci formed in mouse and rat cells under a soft agar overlay. Journal of the National Cancer Institute 46:1001–1007
    [Google Scholar]
  8. Martin G. S. 1970; Rous sarcoma virus: a function required for the maintenance of the transformed state. Nature, London 227:1021–1023
    [Google Scholar]
  9. Parkman R., Levy J. A., Ting R. C. 1970; Murine sarcoma virus: the question of defectiveness. Science 168:387–389
    [Google Scholar]
  10. Rowe W. P., Pugh W. E., Hartley J. W. 1970; Plaque assay techniques for murine leukemia viruses. Virology 42:1136–1139
    [Google Scholar]
  11. Scolnick E. M., Stephenson J. R., Aaronson S. A. 1972; Isolation of temperature-sensitive mutants of murine sarcoma virus. Journal of Virology 10:653–657
    [Google Scholar]
  12. Scolnick E. M., Goldberg R. J., Parks W. P. 1975; A biochemical and genetic analysis of mammalian RNA-containing sarcoma viruses. Cold Spring Harbor Symposia on Quantitative Biology 39:885–895
    [Google Scholar]
  13. Somers K., Kit S. 1973; Temperature-dependent expression of transformation by a cold-sensitive mutant of murine sarcoma virus. Proceedings of the National Academy of Sciences of the United States of America 70:2206–2210
    [Google Scholar]
  14. Stephenson J. R., Aaronson S. A. 1973; Characterization of temperature-sensitive mutants of murine leukemia virus. Virology 54:53–59
    [Google Scholar]
  15. Stephenson J. R., Aaronson S. A. 1974; Temperature-sensitive mutants of murine leukemia virus. III. Mutants defective in helper functions for sarcoma virus fixation. Virology 58:294–297
    [Google Scholar]
  16. Stephenson J. R., Reynolds R. K., Aaronson S. A. 1972; Isolation of temperature-sensitive mutants of murine leukemia virus. Virology 48:749–756
    [Google Scholar]
  17. Svoboda J., Chyle P. P., Simkovic D., Hilgert I. 1963; Demonstration of the absence of infectious Rous virus in rat tumor XC, whose structurally intact cells produce Rous sarcoma when transferred to chicks. Folia Biologica 9:77–81
    [Google Scholar]
  18. Wong P. K. Y., Russ L. J., Mccarter J. A. 1973; Rapid, selective procedure for isolation of spontaneous temperature-sensitive mutants of Moloney leukemia virus. Virology 51:424–431
    [Google Scholar]
  19. Wyke J. A. 1975; Temperature sensitive mutants of avian sarcoma viruses. Biochimica et Biophysica Acta 417:91–121
    [Google Scholar]
  20. Wyke J. A., Linial M. 1973; Temperature-sensitive avian sarcoma viruses: a physiological comparison of twenty mutants. Virology 53:152–161
    [Google Scholar]
  21. Yoshikura H. 1973; Ultraviolet sensitivity of helper function of murine leukemia virus. Archives of Biochemistry and Biophysics 154:76–83
    [Google Scholar]
  22. Yuasa Y., Shimojo H., Aoyagi T., Umezawa H. 1975; Effect of protease inhibitors on focus formation by murine sarcoma virus. Journal of the National Cancer Institute 54:1255–1256
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-36-2-257
Loading
/content/journal/jgv/10.1099/0022-1317-36-2-257
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error