Production of Antiserum to the Reverse Transcriptase of Mason-Pfizer Monkey Virus Free

Abstract

SUMMARY

The reverse transcriptase of Mason-Pfizer monkey virus (M-PMV) has been isolated and partially purified by ion exchange chromatography. Sera from rabbits immunized with the partially purified enzyme have been shown by microimmuno-diffusion analysis to be immunologically specific for the M-PMV polymerase. The immune serum also specifically inhibits M-PMV polymerase activity and this inhibitory activity has been shown to reside in the IgG fraction of the serum. The application of these reagents to examining virus identity and investigating the possible viral aetiology of human breast cancer is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-36-2-227
1977-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/36/2/JV0360020227.html?itemId=/content/journal/jgv/10.1099/0022-1317-36-2-227&mimeType=html&fmt=ahah

References

  1. Aaronson S., Parks W., Scolnick E., Todaro G. 1971; Antibody to the RNA-dependent DNA polymerase of mammalian C type RNA tumor viruses. Proceedings of the National Academy of Sciences of the United States of America 68:920–924
    [Google Scholar]
  2. Abrell J., Gallo R. 1973; Purification, characterization and comparison of the DNA polymerases from two primate RNA tumor viruses. Journal of Virology 12:431–439
    [Google Scholar]
  3. Ahmed M., Mayyasi S., Chopra H., Zelljadt I., Jensen E. 1971; Mason-Pfizer monkey virus isolated from spontaneous mammary carcinoma of a female monkey. I. Detection of virus antigens by immunodiffusion, immunofluorescent and virus agglutination techniques. Journal of the National Cancer Institute 46:1325–1334
    [Google Scholar]
  4. Ahmed M., Scihdlovsky G., Korol W., Vidrine G., Ciemanee J. L. 1974; Occurrence of Mason-Pfizer monkey virus in healthy rhesus monkeys. Cancer Research 34:3504–3508
    [Google Scholar]
  5. Axel R., Gulati S. C., Spiegelman S. 1972; Particles containing RNA-instructed DNA polymerase and virus-related RNA on human breast tumors. Proceedings of the National Academy of Sciences of the United States of America 69:3133–3137
    [Google Scholar]
  6. Chopra H. C., Mason M. L. 1970; A new virus in a spontaneous mammary tumor of a rhesus monkey. Cancer Research 30:2081–2086
    [Google Scholar]
  7. Colcher D., Spiegelman S., Schlom J. 1974; Sequence homology between the RNA of Mason-Pfizer monkey virus and the RNA of human malignant breast tumors. Proceedings of the National Academy of Sciences of the United States of America 71:4975–4979
    [Google Scholar]
  8. Das M. R., Sadasivan E., Koshy R., Vaidya A. B., Sirsat S. M. 1972; Homology between RNA from human malignant breast tissue and DNA synthesized by milk particles. Nature New Biology 239:92–95
    [Google Scholar]
  9. Gallagher R., Todaro G., Smith R., Livingston D., Gallo R. 1974; Relationship between RNA-directed DNA polymerase from human acute leukemic blood cells and primate type-C viruses. Proceedings of the National Academy of Sciences of the United States of America 71:1303–1313
    [Google Scholar]
  10. Gelderblom H., Bauer H., Ogura H., Wigand R., Fischer A. B. 1974; Detection of oncomavirus-like particles in HeLa cells. Fine structure and comparative morphological classification. International Journal of Cancer 13:246–253
    [Google Scholar]
  11. Harewood K., Wolff J. 1973; A rapid electrophoretic procedure for the detection of SDS-released on-comaviral RNA using polyacrylamide-agarose gels. Analytical Biochemistry 55:573–581
    [Google Scholar]
  12. Ilyin K., Bykovsky A., Zhdanov V. 1973; An oncornavirus isolated from human cancer line. Cancer 32:89–96
    [Google Scholar]
  13. Jensen E., Zelljadt I., Chopra H., Mason M. 1970; Isolation and propagation of a virus from a spontaneous mammary carcinoma of a rhesus monkey. Cancer Research 30:2388–2393
    [Google Scholar]
  14. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  15. McGrath C., Grant P., Soule H., Glancy T., Rich M. 1974; Replication of oncornavirus-like particles in human breast carcinoma cell line MCF-7. Nature, London 252:247–250
    [Google Scholar]
  16. Nowinski R., Edynak E., Sarkar N. 1971; Serological and structural properties of Mason-Pfizer monkey virus isolated from the mammary tumor of a rhesus monkey. Proceedings of the National Academy of Sciences of the United States of America 68:1608–1612
    [Google Scholar]
  17. Parks W., Gilden R., Bykovsky A., Miller G., Zhdanov V., Soloviev V., Scolnick E. 1973; MaSOn-Pfizer virus characterization: a similar virus in a human amniotic cell line. Journal of Virology 12:1540–1547
    [Google Scholar]
  18. Scolnick E., Parks W., Todaro G. 1972; Reverse transcriptase of primate viruses as immunological markers. Science 177:1119–1121
    [Google Scholar]
  19. Todaro G., Gallo R. 1973; Immunological relationship of DNA polymerase from human acute leukemia cells and primate and mouse leukemia virus reverse transcriptase. Nature, London 244:206–209
    [Google Scholar]
  20. Yaniv A., Ohno T., Kacian D., Colcher D., Witkin S., Schlom J., Spiegelman S. 1974; Serological analysis of reverse transcriptase of the Mason-Pfizer monkey virus. Virology 59:335–338
    [Google Scholar]
  21. Yeh J., Ahmed M., Mayyasi S., Alessi A. 1975; Detection of an antigen related to Mason-Pfizer virus in malignant human breast tumors. Science 190:583–584
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-36-2-227
Loading
/content/journal/jgv/10.1099/0022-1317-36-2-227
Loading

Data & Media loading...

Most cited Most Cited RSS feed