1887

Abstract

SUMMARY

The physicochemical and serological properties of a virus isolated from the bivalve mollusc, , have been examined. The virus has a diam. of 59 nm, sediments at 430S in sucrose gradients and bands at a density of 1.32 g/ml in CsCl. The virus contains RNA with a mol. wt. about 2.8 × 10 as estimated by poly-acrylamide gel electrophoresis but in sucrose gradients the RNA sediments at 14S. The virus RNA is resistant to ribonuclease under conditions in which ribosomal RNA and the single stranded Mengo virus RNA are completely hydrolysed. Two major polypeptides, mol. wt. 67 and 40 × 10, and one minor polypeptide, mol. wt. 110 × 10, are present in the virus particle. These properties are similar to those found for different serotypes of infectious pancreatic necrosis (IPN) virus. Although there was only a very low level of cross-neutralization between Tellina virus and IPN virus, there was some cross-reaction in immune electron microscopy tests and in immunofluorescence tests with infected tissue culture cells. This cross reaction, together with the close similarity in morphology and physicochemical properties, suggests that Tellina virus and IPN virus belong to the same virus group.

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/content/journal/jgv/10.1099/0022-1317-36-1-93
1977-07-01
2022-01-28
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References

  1. Almeida J. D., Morris R. 1973; Antigenically-related viruses associated with infectious bursal disease. Journal of General Virology 20:369–375
    [Google Scholar]
  2. Brown F., Cartwright B. 1963; Purification of radioactive foot and mouth disease virus. Nature, London 199:1168–1170
    [Google Scholar]
  3. Buchanan J. S. 1973; Electron microscopic observations of virus-like particles in the digestive gland of a marine bivalve mollusc Tellina tenuis. Vlth Annual Meeting of the Society for Invertebrate Pathology p 28
    [Google Scholar]
  4. Cartwright B., Talbot P., Brown F. 1970; The proteins of biologically active sub-units of vesicular stomatitis virus. Journal of General Virology 7:267–272
    [Google Scholar]
  5. Cohen J., Poinsard A., Scherrer R. 1973; Physico-chemical and morphological features of infectious pancreatic necrosis virus. Journal of General Virology 21:485–498
    [Google Scholar]
  6. Cohen J., Scherrer R. 1974; Le virus la necrose pancreatique infectieuse. 1. Purification et structure de la capsid. Annales de Recherches veterinaires 8:87–100
    [Google Scholar]
  7. Dobos P. 1976; Size and structure of the genome of infectious pancreatic necrosis virus. Nucleic Acid Research 3:1903–1924
    [Google Scholar]
  8. Dobos P., Hallett R., Kells D. T. C., Sorensen O., Rowe D. 1977; Biophysical studies of infectious pancreatic necrosis virus (IPNV). Journal of Virology 22:150–159
    [Google Scholar]
  9. Farrell J. A., Harvey J. D., Bellamy A. R. 1974; Biophysical studies of reovirus type 3. 1. The molecular weights of reovirus and reovirus cores. Virology 62:145–153
    [Google Scholar]
  10. Finlay J., Hill B. J. 1975; The use of the complement fixation test for the rapid typing of infectious pancreatic necrosis virus. Aquaculture 5:305–310
    [Google Scholar]
  11. Harkness J. W., Alexander D. J., Pattison M., Scott A. C. 1975; Infectious bursal disease agent morphology by negative stain electron microscopy. Archives of Virology 48:63–73
    [Google Scholar]
  12. Hill B. J. 1976a; Properties of a virus isolated from the bivalve mollusc Tellina tenuis (da Costa). In Wildlife Diseases pp 445–452 Edited by Page L. A. New York and London: Plenum Press;
    [Google Scholar]
  13. Hill B. J. 1976b; Molluscan viruses: their occurrence, culture and relationships. Proceedings of the International Colloquium on Invertebrate Pathology Kingston, Ontario: (in the press)
    [Google Scholar]
  14. Kelly R. K., Loh P. C. 1972; Electron microscopical and biophysical characterisation of IPNV. Journal of Virology 10:824–834
    [Google Scholar]
  15. Lientz J. C., Springer J. E. 1973; Neutralisation tests of infectious pancreatic necrosis virus with polyvalent antiserum. Journal of Wildlife Diseases 9:120–124
    [Google Scholar]
  16. Loh P. C., Lee M. H., Kelly R. K. 1974; The polypeptides of infectious pancreatic necrosis virus. Journal of General Virology 22:421–423
    [Google Scholar]
  17. McDonald R. D., Yamamoto T. 1977; The structure of infectious pancreatic necrosis virus RNA. Journal of General Virology 34:235–247
    [Google Scholar]
  18. Moss L. H., Gravell M. 1969; Ultrastructure and sequential development of infectious pancreatic necrosis virus. Journal of Virology 3:52–58
    [Google Scholar]
  19. Nick H., Cursiefen D., Becht H. 1976; Structural and growth characteristics of infectious bursal disease virus. Journal of Virology 18:227–234
    [Google Scholar]
  20. Piper D., Nicholson B. L., Dunn J. 1973; Immunofluorescent study of the replication of infectious pan-creatic necrosis virus in trout and Atlantic salmon cell cultures. Infection & Immunity 8:249–254
    [Google Scholar]
  21. Vestergaard-Jorgensen P., Grauballe P. C. 1971; Problems in the serological typing of IPN virus. Acta Veterinaria Scandinavica 12:145–147
    [Google Scholar]
  22. Vestergaard-Jorgenson P., Kehlet N. P. 1971; Infectious pancreatic necrosis viruses in Danish rainbow trout. Their serological and pathogenic properties. Nordisk Veterinaermedicin 23:568–575
    [Google Scholar]
  23. Vestergaard-Jorgenson P. 1972; Freund’s adjuvants: their influence on the specificity of viral antisera. Acta pathologica et microbiologica Scandinavica B 80:931–933
    [Google Scholar]
  24. Wilcockson L., Hull R. 1974; The rapid isolation of plant virus RNAs using sodium perchlorate. Journal of General Virology 23:107–111
    [Google Scholar]
  25. Wolf K., Quimby M. C. 1971; Salmonid viruses: infectious pancreatic necrosis virus. Morphology, pathology and serology of first European isolations. Archiv.fur diegesamte Virusforschung 34:144–156
    [Google Scholar]
  26. Wrigley N. G. 1968; The lattice spacing of crystalline catalase as an internal standard length in electron microscopy. Journal of Ultrastructure Research 24:454–464
    [Google Scholar]
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