RT Journal Article SR Electronic(1) A1 Van den Hurk, J. A1 Tas, P. W. L. A1 Peters, D.YR 1977 T1 The Ribonucleic Acid of Tomato Spotted Wilt Virus JF Journal of General Virology, VO 36 IS 1 SP 81 OP 91 DO https://doi.org/10.1099/0022-1317-36-1-81 PB Microbiology Society, SN 1465-2099, AB SUMMARY TSWV nucleic acid, extracted from purified virus with phenol-SDS, was non-infectious. Following electrophoresis of the extracted nucleic acid in 2% poly-acrylamide gels containing 0.5% agarose, 3 major and 2 minor bands were observed. The amount of material contained in the different bands varied with the season. Bands 1, 2, 3 and 4 were sensitive to RNases and resistant to DNase. This was a strong indication of the presence of single-stranded RNA in these bands. Precipitation in 2 m-LiCl, effect of heating and mobility in gels containing an increasing percentage of acrylamide confirmed the single-stranded character. Band 1a was resistant to the action of RNases and stained with Coomassie brilliant blue. The mol. wt. of RNAs 1, 2, 3 and 4 (corresponding to bands 1 to 4) were estimated under both non-denaturing and denaturing conditions and appeared to be 2.5 to 2.7 × 106, 1.9 × 106, 1.7 × 106 and 1.3 × 106, respectively. Following dissociation of the virus with Nonidet P 40, an infectious zone could be isolated after sedimentation in an 8 to 43% glycerol gradient., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-36-1-81