1887

Abstract

SUMMARY

Judged by the yield of infective virus, good infection of cowpea mesophyll protoplasts with alfalfa mosaic virus is obtained when protoplasts are resuspended at 0 °C in 0.5 -mannitol, 0.01 -potassium phosphate, pH 5.6, containing 1 g/ml poly--ornithine and about 2 g/ml virus. At 25°C, virus infectivity is first detectable 12 h after inoculation and increases exponentially in the next 12 h. Forty hours after inoculation about 1 to 5 × 10 virus particles are produced per living protoplast. As with intact leaves, a mixture of bottom component, middle component and top component nucleoprotein is required for the infection of protoplasts.

Virus multiplication is not sensitive to chloramphenicol (200 g/ml) but is completely inhibited by cycloheximide (10 g/ml) applied at any point in the growth cycle. Actinomycin D (10 g/ml) interferes with an early step in virus replication.

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/content/journal/jgv/10.1099/0022-1317-36-1-175
1977-07-01
2024-04-25
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References

  1. Aoki S., Takebe I. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus ribonucleic acid. Virology 39:439–448
    [Google Scholar]
  2. Beier H., Bruening G. 1976; Factors influencing the infection of cowpea protoplasts by cowpea mosaic virus RNA. Virology 72:363–369
    [Google Scholar]
  3. Bol J. F., Bakhuizen C. E. G. C., Rutgers T. 1976; Composition and biosynthetic activity of polyribosomes associated with alfalfa mosaic virus infections. Virology 75:1–17
    [Google Scholar]
  4. Bol J. F., Kraal B., Brederode F. T. 1974; Limited proteolysis of alfalfa mosaic virus: influence on the structural and biological function of the coat protein. Virology 58:101–110
    [Google Scholar]
  5. Bol J. F., Lak-Kaashoek M. 1974; Composition of alfalfa mosaic virus nucleoproteins. Virology 60:476–484
    [Google Scholar]
  6. Bol J. F., Van Vloten-Doting L., Jaspars E. M. J. 1971; A functional equivalence of top component a RNA and coat protein in the initiation of infection by alfalfa mosaic virus. Virology 46:73–85
    [Google Scholar]
  7. Bol J. F., Veldstra H. 1969; Degradation of alfalfa mosaic virus by pancreatic ribonuclease. Virology 37:74–85
    [Google Scholar]
  8. Dawson W. O., Schlegel D. E. 1976; The sequence of inhibition of tobacco mosaic virus synthesis by actinomycin D, 2-thiouracil, and cycloheximide in a synchronous infection. Phytopathology 66:177–181
    [Google Scholar]
  9. Dingjan-Versteegh A., Van Vloten-Doting L., Jaspars E. M. J. 1972; Alfalfa mosaic virus hybrids constructed by exchanging nucleoprotein components. Virology 49:716–722
    [Google Scholar]
  10. Heijtink R. A. 1974; Fysisch-chemische karakterisering van nucleoproteinen en nucieinezuurmoleculen van alfalfa mosaic virus. Ph.D. Thesis University of Leiden;
    [Google Scholar]
  11. Hibi T., Rezelman G., Van Kammen A. 1975; Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64:308–318
    [Google Scholar]
  12. Hull R. 1969; Alfalfa mosaic virus. Advances in Vims Research 15:365–433
    [Google Scholar]
  13. Kassanis B., White R. F., Woods R. D. 1975; Inhibition of multiplication of tobacco mosaic virus in protoplasts by antibiotics and its prevention by divalent metals. Journal of General Virology 28:185–191
    [Google Scholar]
  14. Kleczkowski A. 1950; Interpreting relationships between the concentrations of plant viruses and numbers of local lesions. Journal of General Microbiology 4:53–69
    [Google Scholar]
  15. Kubo S., Harrison B. D., Robinson D. J. 1974; Effect of phosphate on the infection of tobacco protoplasts by tobacco rattle virus. Intervirology 3:382–387
    [Google Scholar]
  16. Kubo S., Robinson D. J., Harrison B. D., Hutcheson A. M. 1976; Uptake of tobacco rattle virus by tobacco protoplasts and the effect of phosphate on infection. Journal of General Virology 30:287–298
    [Google Scholar]
  17. Motoyoshi F., Bancroft J. B., Watts J. W., Burgess J. 1973; The infection of tobacco protoplasts with cowpea chlorotic mottle virus and its RNA. Journal of General Virology 20:177–193
    [Google Scholar]
  18. Motoyoshi F., Hull R., Flack I. H. 1975; Infection of tobacco mesophyll protoplasts by alfalfa mosaic virus. Journal of General Virology 27:263–266
    [Google Scholar]
  19. Motoyoshi F., Watts J. W., Bancroft J. B. 1974; Factors influencing the infection of tobacco protoplasts by cowpea chlorotic mottle virus. Journal of General Virology 25:245–256
    [Google Scholar]
  20. Otsuki Y., Takebe I., Honda Y., Kajita S., Matsui C. 1974; Infection of tobacco mesophyll protoplasts by potato virus X. Journal of General Virology 22:375–385
    [Google Scholar]
  21. Ouchterlony O. 1962; Diffusion-in-gel methods for immunological analysis. II. Progress in Allergy 6:30–154
    [Google Scholar]
  22. Takebe I. 1975; The use of protoplasts in plant virology. Annual Review of Phytopathology 13:105–125
    [Google Scholar]
  23. Van Vloten-Doting L., Dingjan-Versteegh A., Jaspars E. M. J. 1970; Three nucleoprotein components of alfalfa mosaic virus necessary for infectivity. Virology 40:419–430
    [Google Scholar]
  24. Van Vloten-Doting L., Jaspars E. M. J. 1972; The uncoating of alfalfa mosaic virus by its own RNA. Virology 48:699–708
    [Google Scholar]
  25. Verhagen W., Bol J. F. 1972; Evidence for a pH-induced structural change of alfalfa mosaic virus. Virology 50:431–439
    [Google Scholar]
  26. Widholm J. M. 1972; The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technology 47:189–194
    [Google Scholar]
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