The original foot-and-mouth disease virus recombination map (Lake, Priston & Slade, 1975), which included 35 mutagen-induced ts mutants, has been extended both in detail and size by the mapping of a further 33 ts mutants (9 mutagen-induced and 24 spontaneous). The size increase from 0.57% to 3.27% maximum recombination frequency was principally due to the use of a new standardization technique for recombination frequencies but, in addition, the original map distance was increased by approx. 30% due to the mapping of new mutations. As in the original map, there was a marked concentration of mutations near the guanidine (gs) locus, i.e. 83% of the mutants had mutations in the third of the map adjacent to the gs locus.
BlackD. N.,
SangarD. V.,
RowlandsD. J.1975; The biochemical determination of the gene order of foot-and-mouth disease virus. Abstracts of 3rd International Congress of Virology Madrid:
CooperP. D.,
GeisslerE.,
TannockG. A.1975; Attempts to extend the genetic map of poliovirus temperature-sensitive mutants. Journal of General Virology 29:109–120
EpsteinR. H.,
BolleA.,
SteinbergC. M.,
KellenbergerE.,
Boy De La TourE.,
ChevalleyR.,
EdgarR. S.,
SusmanM.,
DenhardtG. H.,
LeilausisA.1963; Physiological studies of conditional lethal mutations of bacteriophage T4D. Cold Spring Harbor Symposium on Quantitative Biology 28:375–394
LakeJ. R.,
MackenzieJ. S.1973; Improved technique for the isolation of temperature-sensitive mutants of foot-and-muth disease virus. Journal of Virology 21:665–668
MackenzieJ. S.,
SladeW. R.,
LakeJ. R.,
PristonR. A. J.,
BisbyJ.,
LaingS.,
NewmanJ. W.1975; Temperature-sensitive mutants of foot-and-mouth disease virus: the isolation of mutants and observations on their properties and genetic recombination. Journal of General Virology 27:61–70
PringleC. R.,
SladeW. R.,
ElworthyP.,
O’sullivanM.1970; Properties of temperature-sensitive mutants of the Kenya 3/57 strain of foot-and-mouth disease virus. Journal of General Virology 6:213–220