The 50S and 35S RNAs from Newcastle Disease Virus-Infected Cells Free

Abstract

SUMMARY

Electrophoretic analyses of the 50S and 35S Newcastle disease virus-specific RNAs from infected cells before and after heat denaturation make it possible to demonstrate that these regions each contain single-stranded RNAs with corresponding S values as well as partially base-paired structures. The partially base-paired structures which sediment at 50S (40 to 60S) have a distribution in gels similar to that of the transcriptive intermediates, and they remain when 50S RNA synthesis (replication) is blocked by cycloheximide. The partially base-paired 35S RNA is more homogeneous and is neither labelled in the transcription reaction nor when infected cells are treated with cycloheximide. These base-paired structures may, therefore, be involved in transcription and replication, respectively.

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1977-06-01
2024-03-29
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References

  1. Bishop D. H. L., Claybrook J. R., Spiegelman S. 1967; Electrophoretic separation of viral nucleic acids on polyacrylamide gels. Journal of Molecular Biology 26:373–387
    [Google Scholar]
  2. Blair C. D., Robinson W. S. 1968; Replication of Sendai virus. I. Comparison of the viral RNA and virus-specific RNA synthesis with Newcastle disease virus. Virology 35:537–549
    [Google Scholar]
  3. Bratt M. A. 1969; RNA synthesis in chick embryo cells infected with different strains of NDV. Virology 38:485–488
    [Google Scholar]
  4. Bratt M. A., Gallaher W. R. 1969; Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus. Proceedings of the National Academy of Sciences of the United States of America 64:536–543
    [Google Scholar]
  5. Bratt M. A., Robinson W. S. 1967; Ribonucleic acid synthesis in cells infected with Newcastle disease virus. Journal of Molecular Biology 23:1–21
    [Google Scholar]
  6. Bratt M. A., Robinson W. S. 1971; Evidence for an RNA intermediate in cells infected with Newcastle disease virus. Journal of General Virology 10:139–145
    [Google Scholar]
  7. Clavell L. A., Bratt M. A. 1971; Relationship between the ribonucleic acid-synthesizing capacity of ultraviolet-irradiated Newcastle disease virus and its ability to induce interferon. Journal of Virology 8:500–508
    [Google Scholar]
  8. Collins B. S., Bratt M. A. 1973; Separation of the messenger RNAs of Newcastle disease virus by gel electrophoresis. Proceedings of the National Academy of Sciences of the United States of America 70:2544–2548
    [Google Scholar]
  9. Hightower L. H., Bratt M. A. 1974; Protein synthesis in Newcastle disease virus-infected chicken embryo cells. Journal of Virology 13:788–800
    [Google Scholar]
  10. Hightower L. E., Morrison T. G., Bratt M. A. 1975; Relationships among the polypeptides of Newcastle disease virus. Journal of Virology 16:1599–1607
    [Google Scholar]
  11. Huang A. S., Baltimore D., Bratt M. A. 1971; Ribonucleic acid polymerase in virions of Newcastle disease virus: comparison with the vesicular stomatitis virus polymerase. Journal of Virology 7:389–394
    [Google Scholar]
  12. Kaverin N. V., Varich N. L. 1974; Newcastle disease virus-specific RNA: polyacrylamide gel analysis of single-stranded RNA and hybrid duplexes. Journal of Virology 13:253–260
    [Google Scholar]
  13. Kingsbury D. W. 1966; Newcastle disease virus RNA. II. Preferential synthesis of RNA complementary to parental viral RNA by chick embryo cells. Journal of Molecular Biology 18:204–214
    [Google Scholar]
  14. Kingsbury D. W. 1973; Cell-free translation of paramyxovirus messenger RNA. Journal of Virology 12:1020–1027
    [Google Scholar]
  15. Kolakofsky D., Boy De La Tour E., Bruschi A. 1974; Self-annealing of Sendai virus RNA. Journal of Virology 14:33–39
    [Google Scholar]
  16. Kolakofsky D., Boy De La Tour E., Delius H. 1974; Molecular weight determination of Sendai and Newcastle disease virus RNA. Journal of Virology 13:261–268
    [Google Scholar]
  17. Morrison T. G., Weiss S., Hightower L. E., Spanier-Collins B., Bratt M. A. 1975; Newcastle disease virus protein synthesis. Institut National de la Sante et de la Recherche Medicale 47:281–290
    [Google Scholar]
  18. Oberg B., Philipson L. 1971; Replicative structures of poliovirus RNA in vivo. Journal of Molecular Biology 58:725–737
    [Google Scholar]
  19. Peacock A. C., Dingman C. W. 1968; Molecular weight estimates and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7:668–674
    [Google Scholar]
  20. Portner A., Kingsbury D. W. 1972; Identification of transcriptive and replicative intermediates in Sendai virus-infected cells. Virology 47:711–725
    [Google Scholar]
  21. Robinson W. S. 1971; Sendai virus RNA synthesis and nucleocapsid formation in the presence of cyclo-heximide. Virology 44:494–502
    [Google Scholar]
  22. Roux L., Kolakofsky D. 1975; Isolation of RNA transcripts from the entire Sendai viral genome. Journal of Virology 16:1426–1434
    [Google Scholar]
  23. Schincariol A. L., Howatson A. F. 1972; Replication of vesicular stomatitis virus. II. Separation and characterization of virus-specific RNA species. Virology 49:766–783
    [Google Scholar]
  24. Spanier-Collins B. 1975; Characterization of Newcastle disease virus-specific RNAs. Ph.D. Thesis Harvard University;
    [Google Scholar]
  25. Weiss S. R. 1976; Analysis of Newcastle disease virus messenger RNA and poly (A). Ph.D. Thesis Harvard University;
    [Google Scholar]
  26. Weiss S. R., Bratt M. A. 1974; Polyadenylate sequences on Newcastle disease virus RNA synthesized in vivo and in vitro. Journal of Virology 13:1220–1230
    [Google Scholar]
  27. Weiss S. R., Bratt M. A. 1975; The effect of cordycepin 3′-deoxyadenosine on virus specific RNA species synthesized in Newcastle disease virus infected cells. Journal of Virology 116:1575–1583
    [Google Scholar]
  28. Weiss S. R., Bratt M. A. 1976; The 18-22S RNAs of Newcastle disease virus. Journal of Virology 18:316–323
    [Google Scholar]
  29. Zhdanov V. M., Kingsbury D. W. 1969; Replicative intermediate of Newcastle disease virus. Voprosy Virusologyii 5:537–541
    [Google Scholar]
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