RT Journal Article SR Electronic(1) A1 Spanier, Bonnie B. A1 Bratt, Michael A.YR 1977 T1 The 50S and 35S RNAs from Newcastle Disease Virus-Infected Cells JF Journal of General Virology, VO 35 IS 3 SP 439 OP 453 DO https://doi.org/10.1099/0022-1317-35-3-439 PB Microbiology Society, SN 1465-2099, AB SUMMARY Electrophoretic analyses of the 50S and 35S Newcastle disease virus-specific RNAs from infected cells before and after heat denaturation make it possible to demonstrate that these regions each contain single-stranded RNAs with corresponding S values as well as partially base-paired structures. The partially base-paired structures which sediment at 50S (40 to 60S) have a distribution in gels similar to that of the in vitro transcriptive intermediates, and they remain when 50S RNA synthesis (replication) is blocked by cycloheximide. The partially base-paired 35S RNA is more homogeneous and is neither labelled in the in vitro transcription reaction nor when infected cells are treated with cycloheximide. These base-paired structures may, therefore, be involved in transcription and replication, respectively., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-35-3-439