1887

Abstract

SUMMARY

The mycovirus infecting has been fractionated into two particle classes designated A and B. Particles A have a buoyant density of 1.385 g/cm, in CsCl, a sedimentation coefficient of 75S and contain one major and two minor polypeptides with molecular weights of 34000, 31000 and 28000, respectively. Particles B band at 1.335 g/cm, sediment with a value of 67S and, in addition to the three polypeptides as in particles A, they contain seven extra polypeptides, six of which are of lower molecular weights. Both particles A and B contain double-stranded RNA which was resolved by electrophoresis on polyacrylamide gels into three components with molecular weights of 1.4 (major), 2.5 and 1.1 × 10 (minor). Both particles also contain single-stranded RNA. Particles A contain 27% RNA whereas particles B only contain 14%. Evidence is presented that particles A and B belong to the same type of virus and represent two different replicative stages.

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1977-06-01
2024-11-03
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References

  1. Barlow J. J., Mathias A. P., Williamson R., Gammak D. B. 1963; A simple method for the quantitative isolation of undegraded high molecular weight ribonucleic acid. Biochemical and Biophysical Research Communications 13:61–66
    [Google Scholar]
  2. Britten R. J., Pavich M., Smith J. 1969; A new method for DNA purification. In Carnegie Institution of Washington Yearbook 1968: pp 400–402
    [Google Scholar]
  3. Buck K. W., Kempson-Jones G. F. 1973; Biophysical properties of Penicillium stoloniferum virus S. Journal of General Virology 18:223–235
    [Google Scholar]
  4. Buck K. W., Ratti G. 1975; Biophysical and biochemical properties of two viruses isolated from Aspergillus foetidus. Journal of General Virology 27:211–224
    [Google Scholar]
  5. Burnett J. P., Frank B. H., Douthart R. J. 1975; Ionic strength effects on the stability and conformation of Penicillium chrysogenum mycophage double-stranded RNA. Nucleic Acids Research 2:759–771
    [Google Scholar]
  6. Cox R. A., Kanagalingam K., Sutherland E. S. 1970; Double-helical character of ribonucleic acid from virus-like particles found in Penicillium chrysogenum. Biochemical Journal 120:549–557
    [Google Scholar]
  7. Dische Z. 1955; Color reactions of nucleic acid components. In The Nucleic Acids vol I p 285 Edited by Chargaff E., Davidson J. N. New York: Academic Press Inc;
    [Google Scholar]
  8. Fähnrich P., Rothe H. H., Trapp M. 1975; Isolation and characterization of ribosomal RNA from the phycomycete Allomyces arbuscula. Zeitschrift für Naturforschung 30:302–303
    [Google Scholar]
  9. Hollings M., Stone O. M. 1971; Viruses that infect fungi. Annual Review of Phytopathology 9:93–118
    [Google Scholar]
  10. Khandjian E.-W., Roos U. P., Timberlake W. E., Eder L., Turian G. 1974; RNA virus-like particles in the chytridiomycete Allomyces arbuscula. Archives for Microbiology 101:351–356
    [Google Scholar]
  11. Khandjian E. W., Roos U.-P., Turian G. 1975; Mycovirus d’Allomyces. Pathologia et Microbiologia 42:250–251
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Lane L. C., Kaesberg P. 1971; Multiple genetic components in bromegrass mosaic virus. Nature New Biology 232:40–43
    [Google Scholar]
  14. Lemke P. A., Nash C. H. 1974; Fungal viruses. Bacteriological Review 38:29–56
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. Mandel M., Marmur J. 1968; Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. In Methods in Enzymology vol XIIB p 195 Edited by Grossman L., Moldave K. New York: Academic Press Inc;
    [Google Scholar]
  17. Nash C. H., Douthart R. J., Ellis L. F., Van Frank R. M., Burnett J. P., Lemke P. A. 1973; On the mycophage of Penicillium chrysogenum. Canadian Journal of Microbiology 19:97–103
    [Google Scholar]
  18. Ojha M., Turian G. 1971; Interspecific transformation and DNA characteristics in Allomyces. Molecular and General Genetics 112:49–59
    [Google Scholar]
  19. Peacock A. C., Dingman C. W. 1968; Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels. Biochemistry 7:668–674
    [Google Scholar]
  20. Pinck L., Hirth L., Bernardi G. 1968; Isolation of replicative RNA from alfalfa mosaic virus infected plants by chromatography on hydroxylapatite columns. Biochemical and Biophysical Research Communications 31:481–487
    [Google Scholar]
  21. Rawlinson C. J., Hornby D., Pearson V., Carpenter J. M. 1973; Virus-like particles in the take-all fungus Gaeumannomyces graminis. Annals of Applied Biology 74:197–209
    [Google Scholar]
  22. Rawlinson C. J., Carpenter J. M., Muthyalu G. 1975; Double-stranded RNA virus in Colletotrichum lindemuthianum. Transactions of the British Mycological Society 65:305–308
    [Google Scholar]
  23. Roos U.-P., Khandjian E. W., Turian G. 1976; RNA virus in Allomyces arbuscula: ultrastructural localization during the life cycle. Journal of General Microbiology 95:87–95
    [Google Scholar]
  24. Szybalski W. 1968; Use of caesium sulfate for equilibrium density gradient centrifugation. In Methods in Enzymology vol XIIB p 330 Edited by Grossman L., Moldave K. New York: Academic Press Inc;
    [Google Scholar]
  25. Turian G. 1963; Synthese differentielle d’acide ribonucleique et differenciation sexuelle chez V Allomyces. Developmental Biology 6:61–72
    [Google Scholar]
  26. Van Franck R. M., Ellis L. F., Kleinschmidt W. J. 1971; Purification and physical properties of mycophage PS 1. Journal of General Virology 12:33–42
    [Google Scholar]
  27. Wood H. A. 1973; Viruses with double-stranded RNA genomes. Supplement Symposium Journal of General Virology 20:61–85
    [Google Scholar]
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