1887

Abstract

SUMMARY

Human leukocyte interferon, purified approximately 1000-fold by affinity chromatography on immobilized anti-interferon globulins and SDS-Sephadex filtration, was resolved into one major and one minor component by adsorption chromatography on hydroxylapatite and electrophoresis in polyacrylamide gels. These components were indistinguishable in their capacity to protect bovine, porcine and murine cells, and the antiviral activities of both were equally susceptible to reduction by β-mercaptoethanol. They were neutralized to the same degree by rabbit anti-leukocyte interferon but were not neutralized by rabbit antifibroblast interferon serum. Mice immunized with either component developed antibodies to both but failed to form antibodies against human fibroblast interferon. Our present evidence indicates that the two components possess at most only minor structural and antigenic dissimilarities.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-35-2-341
1977-05-01
2022-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/35/2/JV0350020341.html?itemId=/content/journal/jgv/10.1099/0022-1317-35-2-341&mimeType=html&fmt=ahah

References

  1. Anfinsen C. B., Bose S., Corley L., Gurari-Rotman D. 1974; Partial purification of human interferon by affinity chromatography. Proceedings of the National Academy of Sciences of the United States of America 71:3139–3142
    [Google Scholar]
  2. Berg K., Ogburn C. A., Paucker K., Mogensen K. E., Cantell K. 1975; Affinity chromatography of human leukocyte and diploid cell interferons on Sepharose-bound antibodies. The Journal oflmmunology 114:640–644
    [Google Scholar]
  3. Borecký L., Fuchsberger N., Hajnická V. 1974; Electrophoretic profiles and activities of human interferon in heterologous cells. Intervirology 3:369–377
    [Google Scholar]
  4. Cantell K. 1970; Preparation of human leukocyte interferon. In Symposia Series in Immunological Standardization. International Symposium on Interferon and Interferon Inducers vol 14 pp 6–8 Edited by Perkins F. T., Regamey R. H. Basel: S. Karger AG;
    [Google Scholar]
  5. Cantell K., Hirvonen S., Mogensen K. E., Pyhälä L. 1974; Human leukocyte interferon: production, purification, stability, and animal experiments. In The production and use of interferon for the treatment and prevention of human virus infections pp 35–38In Vitro Monograph no. 3. Edited by Waymouth C. Rockville: The Tissue Culture Association;
    [Google Scholar]
  6. Cantell K., Pyhälä L. 1973; Circulating interferon in rabbits after administration of human interferon by different routes. Journal of General Virology 20:97–104
    [Google Scholar]
  7. Chen J. K., Jankowski W. J., O’Malley J. A., Sulkowski E., Carter W. A. 1976; The nature of the molecular heterogeneity of human leukocyte interferon. Journal of Virology 19:425–434
    [Google Scholar]
  8. Cuatrecasas P., Anfinsen C. B. 1971; Affinity chromatography. Separation based on specific activity. In Methods in Enzymology vol 22 pp 345–378 Edited by Jacoby W. B. New York: Academic Press;
    [Google Scholar]
  9. Desmyter J., Stewart W. E. II. 1976; Molecular modification of interferon: attainment of human interferon in a conformation active on cat cells but inactive on human cells. Virology 70:451–458
    [Google Scholar]
  10. Epstein L. B., Kreth H. W., Herzenberg L. A. 1974; Fluorescence-activated cell sorting of human T and B lymphocytes. II. Identification of the cell type responsible for interferon production and cell proliferation in response to mitogens. Cellular Immunology 12:407–421
    [Google Scholar]
  11. Fantes K. H. 1970a; Purification and properties of human interferon. Annals of the New York Academy of Sciences 173:118–121
    [Google Scholar]
  12. Fantes K. H. 1970b; Partial purification, concentration and properties of human leukocyte interferon. In Colloques de Vlnstitut National de la Sante et de la Recherche Medicate. L’Interferon no. 6, pp 181–186 Paris: Institut National de la Sante et de la Recherche Medicale;
    [Google Scholar]
  13. Fantes K. H. 1974; Human leukocyte interferon: properties and purification. In The production and use of interferon for the treatment and prevention of human virus infections p 48In Vitro Monograph no. 3. Edited by Waymouth C. Rockville: The Tissue Culture Association;
    [Google Scholar]
  14. Fantes K. H., Furminger I. G. S. 1967; Polyacrylamide gel electrophoresis of highly purified chick interferon. Nature, London 216:71–72
    [Google Scholar]
  15. Gresser I., Bandu M.-T., Brouty-Boyé D., Tovey M. 1974; Pronounced antiviral activity of human interferon on bovine and procine cells. Nature, London 251:543–545
    [Google Scholar]
  16. Havell E. A., Vilček J. 1972; Production of high-titered interferon in cultures of human diploid cells. Antimicrobial Agents and Chemotherapy 2:476–484
    [Google Scholar]
  17. Havell E. A., Berman B., Ogburn C. A., Berg K., Paucker K., Vilček J. 1975a; TWO antigenically distinct species of human interferon. Proceedings of the National Academy of Sciences of the United States of America 72:2185–2187
    [Google Scholar]
  18. Havell E. A., Berman B., Vilček J. 1975b; Antigenic and biologic differences between human leukocyte and fibroblast interferons. Ninth International Immunobiological Symposium, Yugoslav Academy of Sciences and Arts, Zagreb, October1–2 (in the press)
    [Google Scholar]
  19. Levy-Koenig R. E., Golgher R. R., Paucker K. 1970; Immunology of interferons. II. Heterospecific activities of human interferon and their neutralization by antibody. The Journal of Immunology 104:791–797
    [Google Scholar]
  20. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  21. Matsuo A., Hayashi S., Kishida T. 1974; Production and purification of human leukocyte interferon. Japanese Journal of Microbiology 18:21–27
    [Google Scholar]
  22. McClurkin A. W., Pirtle E. C., Coria M. F., Smith R. L. 1974; Comparison of low- and high-passage bovine turbinate cells for assay of bovine viral diarrhea virus. Archiv fiir die gesamte Virusforschung 45:285–289
    [Google Scholar]
  23. Mogensen K. E., Cantell K. 1974; Human leukocyte interferon: a role for disulphide bonds. Journal of General Virology 22:95–103
    [Google Scholar]
  24. Mogensen K. E., Pyhälä L., Cantell K. 1975; Raising antibodies to human leukocyte interferon. Acta Pathologica et Microbiologica Scandinavica Section B 83:443–450
    [Google Scholar]
  25. Mogensen K. E., Pyhälä L., Torma E., Cantell K. 1974; No evidence for a carbohydrate moiety affecting the clearance of circulating human leukocyte interferon in rabbits. Acta Pathologica et Microbiologica Scandinavica Section B 82:305–310
    [Google Scholar]
  26. Moss B., Rosenblum E. N. 1972; Hydroxylapatite chromatography of protein-sodium dodecyl sulfate complexes. Journal of Biological Chemistry 247:5194–5198
    [Google Scholar]
  27. Ogburn C. A., Berg K., Paucker K. 1973; Purification of mouse interferon by affinity chromatography on anti-interferon globulin-Sepharose. The Journal of Immunology m:1206–1218
    [Google Scholar]
  28. Paucker K., Dalton B. J., Ogburn C. A., Törmä E. 1975; Multiple active sites on human interferons. Proceedings of the National Academy of Sciences of the United States of America 72:4587–4591
    [Google Scholar]
  29. Riley V. 1960; Adaptation of orbital bleeding technic to rapid serial blood studies. Proceedings of the Society for Experimental Biology and Medicine 104:751–754
    [Google Scholar]
  30. Salvin S. B., Younger J. S., Nishio J., Neta R. 1975; Brief communication: tumor suppression by a lymphokine released into the circulation of mice with delayed hypersensitivity. Journal of the National Cancer Institute 55:1233–1236
    [Google Scholar]
  31. Sanford K. K., Earle W. R., Likely G. D. 1948; The growth in vitro of single isolated tissue cells. Journal of the National Cancer Institute 9:229–246
    [Google Scholar]
  32. Stanček D., Gressnerová M., Paucker K. 1970; Isoelectric components of mouse, human and rabbit interferons. Virology 41:740–750
    [Google Scholar]
  33. Stewart W. E. II, Desmyter J. 1975; Molecular heterogeneity of human leukocyte interferon: two populations differing in molecular weights, requirements for renaturation, and cross-species antiviral activity. Virology 67:68–73
    [Google Scholar]
  34. Strander H., Cantell K. 1966; Production of interferon by human leukocytes in vitro. Annales Medicinae Experimentalis et Biologiae Fenniae 44:265–273
    [Google Scholar]
  35. Törmä E. T., Paucker K. 1976; Purification and characterization of human leukocyte interferon components. Journal of Biological Chemistry 251:4810–4816
    [Google Scholar]
  36. Weber K., Osborn M. 1969; The reliability of molecular weight determinations by dodecyl sulfate polyacrylamide gel electrophoresis. Journal of Biological Chemistry 244:4406–4412
    [Google Scholar]
  37. Youngner J. S., Salvin S. B. 1973; Production and properties of migration inhibitory factor and interferon in the circulation of mice with delayed hypersensitivity. The Journal of Immunology 111:1914–1922
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-35-2-341
Loading
/content/journal/jgv/10.1099/0022-1317-35-2-341
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error