1887

Abstract

SUMMARY

Forty-six arboviruses were tested for c.p.e. and/or plaque formation in an amphibian cell line. C.p.e. was observed with a high proportion of the viruses tested. Comparative plaque assay, in the XTC-2 cells at 28 °C and Vero cells at 37 °C, suggests that these systems are comparable in sensitivity and susceptibility to infection. Practical uses of this cell line are discussed.

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/content/journal/jgv/10.1099/0022-1317-35-2-335
1977-05-01
2021-09-25
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References

  1. Chanas A. C., Johnson B. K., Simpson D. I. H. 1976; Antigenic relationships of alphaviruses by a simple microculture cross-neutralization method. Journal of General Virology 33:295–300
    [Google Scholar]
  2. Cunningham C. H. 1973; Quantal and enumerative titration of virus in cell cultures. In Tissue Culture Methods and Applications pp 527–532 Edited by Kruse Paul F. Jun, Patterson M. K. New York: Academic Press;
    [Google Scholar]
  3. DeMadrid A. T., Porterfield J. S. 1969; A simple micro-culture method for the study of Group B arboviruses. Bulletin of the World Health Organization 40:113–121
    [Google Scholar]
  4. Haldane J. B. S. 1960; ‘Dex’ or ‘order of magnitude’?. Nature, London 187:879
    [Google Scholar]
  5. Johnson B. K. 1973; Arbovirus and arbovirus-antibody complexes in mosquitoes and mosquito cell culture. Ph.D. thesis University of London;
    [Google Scholar]
  6. Leake C. J. 1975; Plaque production by some arboviruses in a cell line from the South African clawedtoad, Xenopus laevis. Transactions of the Royal Society of Tropical Medicine and Hygiene 69:436
    [Google Scholar]
  7. Leibovitz A. 1963; The growth and maintenance of tissue cell cultures in free gas exchange with the atmosphere. American Journal of Hygiene 78:173–180
    [Google Scholar]
  8. Pudney M. 1973 Cell culture of tissues from arthropods and lower vertebrates for application in the study of arboviruses Ph.D. thesis University of London;
    [Google Scholar]
  9. Pudney M., Varma M. G. R., Leake C. J. 1973; Establishment of a cell line from the South African clawed toad, Xenopus laevis. Experientia 29:466–467
    [Google Scholar]
  10. Reeves W. C. 1961; Overwintering of arthropod-borne viruses. Progress in Medical Virology 3:59–78
    [Google Scholar]
  11. Thompson W. R. 1947; Use of moving averages and interpolation to estimate median effective dose. Bacteriological Reviews 11:115–145
    [Google Scholar]
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