1887

Abstract

SUMMARY

The indirect ferritin-labelled antibody technique was used to determine the reactivity of an antiserum prepared against the NZB xenotropic virus with three murine xenotropic viruses, a feline xenotropic virus and a murine ecotropic virus. The envelope antigens of the xenotropic type C viruses isolated from the NZB, NIH Swiss and C57L mice were tagged with ferritin. The feline RD114 virus was not. Gross murine leukaemia virus was tagged, but only at high serum concentrations. The cross-reactivity titre of Gross virus to anti-NZB serum was removed by a serum dilution which was still reactive to xenotropic viruses. This difference in reactivity titres between a xenotropic and an ecotropic virus was sufficient to distinguish one from the other in doubly infected cultures. Specific tagging of membranes of cells infected by xenotropic virus was also observed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-35-2-317
1977-05-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/35/2/JV0350020317.html?itemId=/content/journal/jgv/10.1099/0022-1317-35-2-317&mimeType=html&fmt=ahah

References

  1. Aaronson S. A., Stephenson J. R. 1974; Widespread natural occurrence of high-titered neutralizing antibodies to a specific class of endogenous mouse type C virus. Proceedings of the National Academy of Sciences of the United States of America 71:1957–1961
    [Google Scholar]
  2. Aoki T., Todaro O. J. 1973; Antigenic properties of endogenous type-C viruses from spontaneously transformed clones of BALB/3T3. Proceedings of the National Academy of Sciences of the United States of America 70:1598–1602
    [Google Scholar]
  3. Arnstein P., Levy J. A., Oshiro L. S., Price P. J., Suk W., Lennette E. H. 1974; Recovery of murine xenotropic type-C virus from G57L mice. Journal of the National Cancer Institute 53:1787–1792
    [Google Scholar]
  4. Cremer N. E., Taylor D. O. N., Lennette E. H. 1969; Localization of immunoglohulin and viral antigen in rats infected with Moloney virus. Journal of the National Cancer Institute 42:695–705
    [Google Scholar]
  5. Gross L. 1951; Pathogenic properties, and ‘vertical’ transmission of the mouse leukemia agent. Proceedings of the Society for Experimental Biology and Medicine 78:342–348
    [Google Scholar]
  6. Hanna M. G. Jun, Ihle J. N., Batzing B. L., Tennant R. W., Schenley C. K. 1975; Assessment of reactivities of natural antibodies to endogenous RNA tumor virus envelope antigens and virus-induced cell surface antigens. Cancer Research 35:164–171
    [Google Scholar]
  7. Hartley J. W., Rowe W. P. 1976; Naturally occurring murine leukemia viruses in wild mice: characterization of new ‘amphotropic’ class. Journal of Virology 19:19–25
    [Google Scholar]
  8. Hino S., Stephenson J. R., Aaronson S. A. 1976; Radioimmunoassays for the 70000-molecular-weight glycoproteins of endogenous mouse type C viruses: viral antigen expression in normal mouse tissues and sera. Journal of Virology 18:933–941
    [Google Scholar]
  9. Lee J. C., Hanna M. G. Jun, Ihle J. N., Aaronson S. A. 1974; Autogenous immunity to endogenous RNA tumor virus: differential reactivities of immunoglobulins M and G to virus envelope antigens. Journal of Virology 14:773–781
    [Google Scholar]
  10. Levy J. A. 1973; Xenotropic viruses: muiine leukemia viruses associated with NIH Swiss, NZB, and other mouse strains. Science 182:1151–1153
    [Google Scholar]
  11. Levy J. A. 1974; Autoimmunity and neoplasia. The possible role of C-type viruses. American Journal of Clinical Pathology 62:258–280
    [Google Scholar]
  12. Levy J. A. 1975; Xenotropic C-type viruses and autoimmune disease. Journal of Rheumatology 2:135–148
    [Google Scholar]
  13. Levy J. A., Kazan P., Varnier O., Kleiman H. 1975; Murine xenotropic C-type viruses. I. Distribution and further characterization of the virus in NZB mice. Journal of Virology 16:844–853
    [Google Scholar]
  14. Levy J. A., Pincus T. 1970; Demonstration of biological activity of a murine leukemia virus of New Zealand black mice. Science 170:326–327
    [Google Scholar]
  15. Mcallister R. M., Nicolson M., Gardner M. B., Rongey R. W., Rasheed S., Sarma P. S., Huebner R. J., Hatanaka M., Oroszlan S., Gilden R. V., Kabigting A., Vernon L. 1972; C-type virus released from cultured human rhabdomyosarcoma cells. Nature New Biology 235:3–6
    [Google Scholar]
  16. Rifkind R. A., Hsu K. C., Morgan C. 1964; Immunochemical staining for electron microscopy. Journal of Histochemistry and Cytochemistry 12:131–136
    [Google Scholar]
  17. Singer S. J. 1959; Preparation of an electron-dense antibody conjugate. Nature, London 183:1523–1524
    [Google Scholar]
  18. Stephenson J. R., Aaronson S. A., Arnstein P., Huebner R. J., Tronick S. R. 1974; Demonstration of two immunologically distinct xenotropic type C RNA viruses of mouse cells. Virology 61:56–63
    [Google Scholar]
  19. Stewart M. L., Maizel J. V. Jun 1975; Friend leukemia and endogenous viruses. I. Production of Friend and endogenous C-type particles by mouse cells cultured in vitro. Virology 65:55–66
    [Google Scholar]
  20. Todaro O. J., Arnstein P., Parks W. P., Lennette E. H., Huebner R. J. 1973; A type-C virus in human rhabdomyosarcoma cells after inoculation into NIH Swiss mice treated with antithymyocyte serum. Proceedings of the National Academy of Sciences of the United States of America 70:859–962
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-35-2-317
Loading
/content/journal/jgv/10.1099/0022-1317-35-2-317
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error