1887

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Volume 35, Issue 2

The Uncoupled Relationship between the Temperature-sensitivity and Neurovirulence in Mice of Mutants of Vesicular Stomatitis Virus Free

Abstract

SUMMARY

Inoculation of wild-type (wt) VSV intracerebrally (i.c.) in Swiss weanling mice results in a rapidly fatal illness with death in two to three days. In contrast, i.c. inoculation of temperature-sensitive () VSV mutants G31 and G22, but not G11 or G41, results in a more slowly progressive central nervous system (CNS) disease with distinct neurological signs. Studies undertaken to evaluate the neurovirulence of VSV mutants indicated that the ability of mutants to produce pathological changes in the CNS of mice appeared related to their ability to replicate to high titre in brain and spinal cord. However, replication of VSV mutants in brain alone was not sufficient to produce clinical illness. More importantly, the ability of VSV mutants to replicate at non-permissive temperatures did not appear to correlate with neurovirulence. VSV harvests from brains and spinal cords of mice infected with each of the mutants were temperature-insensitive. In spite of their temperature-insensitivity, the biological behaviour of viruses recovered from CNS tissues was, surprisingly, not that which was characteristic of revertant clones. Virus isolates recovered from infected CNS tissues, despite their temperature-insensitivity, behaved biologically like the original stocks of mutant virus. These data suggest that temperature-sensitivity is not directly correlated with the unique pathogenesis elicited by infection with VSV mutants.

  • Received:
  • Accepted:
  • Published Online:
© Journal of General Virology 1977
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1977-05-01
2024-04-25
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References

  1. Burge B. W., Pfefferkorn E. R. 1966; Isolation and characterization of conditional-lethal mutants of Sindbis virus. Virology 30:204–213
     [Google Scholar]
  2. Clark H. F., Koprowski H. 1971; Isolation of temperature-sensitive conditional-lethal mutants of ‘fixed’ rabies virus. Journal of Virology 7:295–300
     [Google Scholar]
  3. Clark H. F., Wiktor T. J. 1972; Temperature-sensitivity characteristics distinguishing substrains of fixed rabies virus: lack of correlation with plaque-size markers on virulence for mice. Journal of Infectious Diseases 125:637–645
     [Google Scholar]
  4. Dal Canto M. C., Rabinowitz S. G., Johnson T. C. 1976a; Status spongiosus resulting from intracerebral infection of mice with temperature-sensitive mutants of vesicular stomatitis virus. British Journal of Experimental Pathology 57:321–329
     [Google Scholar]
  5. Dal Canto M. C., Rabinowitz S. G., Johnson T. C. 1976b; An ultrastructural study of central nervous system disease produced by wild-type and temperature-sensitive mutants of vesicular stomatitis virus. Laboratory Investigation 35:185–196
     [Google Scholar]
  6. Doyle M., Holland J. J. 1973; Prophylaxis and immunization in mice by use of virus-free defective T particles to protect against intracerebral infection by vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 70:2105–2108
     [Google Scholar]
  7. Haspel M. V., Duff R., Rapp F. 1975; Experimental measles encephalitis: a genetic analysis. Infection and Immunity 12:785–790
     [Google Scholar]
  8. Holland J. J., Villarreal L. P. 1974; Persistent non-cytocidal vesicular stomatitis virus infections mediated by defective T particles that suppress virion transcriptase. Proceedings of the National Academy of Sciences of the United States of America 71:2956–2960
     [Google Scholar]
  9. Huang A. S., Wagner R. R. 1966; Comparative sedimentation coefficients of RNA extracted from plaque-forming and defective particles of vesicular stomatitis virus. Journal of Molecular Biology 22:381–384
     [Google Scholar]
  10. Leamnson R. N., Reichmann M. E. 1974; The RNA of defective vesicular stomatitis virus particles in relation to viral cistrons. Journal of Molecular Biology 85:551–568
     [Google Scholar]
  11. Mills J., Chanock R. M. 1971; Temperature-sensitive mutants of influenza virus. I. Behavior in tissue culture and in experimental animals. Journal of Infectious Diseases 123:145–157
     [Google Scholar]
  12. Preble O. T., Youngner J. S. 1975; Temperature-sensitive viruses and the etiology of chronic and inapparent infections. Journal of Infectious Diseases 131:467–473
     [Google Scholar]
  13. Pringle C. R. 1970; Genetic characteristics of conditional-lethal mutants of vesicular stomatitis virus induced by 5-fluorouracil, 5-azacytidine, and ethyl methane sulfonate. Journal of Virology 5:559–567
     [Google Scholar]
  14. Pringle C. R., Duncan I. B. 1971; Preliminary physiological characterization of temperature-sensitive mutants of vesicular stomatitis virus. Journal of Virology 8:56–61
     [Google Scholar]
  15. Rabinowitz S. G., Dal Canto M. C., Johnson T. C. 1976; Comparison of central nervous system disease produced by wild-type and temperature-sensitive mutants of vesicular stomatitis virus. Infection and Immunity 13:1242–1249
     [Google Scholar]
  16. Reed L. J., Muench H. 1938; A simple method for estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
     [Google Scholar]
  17. Simizu B., Takayama N. 1971; Relationship between neurovirulence and temperature-sensitivity of an attempted western equine encephalitis virus. Archiv fur die Gesamte Virusforschung 35:242–250
     [Google Scholar]
  18. Simpson R. W., Hirst G. K. 1968; Temperature-sensitive mutants of influenza A virus: isolation of mutants and preliminary observations on genetic recombination and complementation. Virology 35:41–49
     [Google Scholar]
  19. Stanners C. P., Goldberg V. J. 1975; On the mechanism of neurotropism of vesicular stomatitis virus in newborn hamsters. Studies with temperature-sensitive mutants. Journal of General Virology 29:286–296
     [Google Scholar]
  20. Tarr G. C., Lubiniecki A. S. 1976; Chemically-induced temperature-sensitive mutants of dengue virus type 2: comparison of temperature-sensitivity in vitro with infectivity in suckling mice, hamsters, and rhesus monkeys. Infection and Immunity 13:688–695
     [Google Scholar]
  21. Youngner J. S., Quagliana D. O. 1976; Temperature-sensitive mutants of vesicular stomatitis virus are conditionally defective particles that interfere with and are rescued by wild-type virus. Journal of Virology 19:102–107
     [Google Scholar]
  22. Zygraich N., Huygelen C. 1973; In vivo behavior of a temperature-sensitive (ts) mutants of herpesvirus hominis type 2. Archiv für die Gesamte Virusforschung 43:103–111
     [Google Scholar]
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The Uncoupled Relationship between the Temperature-sensitivity and Neurovirulence in Mice of Mutants of Vesicular Stomatitis Virus
J. Gen. Virol. 35, 237 (1977); https://doi.org/10.1099/0022-1317-35-2-237
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