1887

Abstract

SUMMARY

The expression of virus, thymus and tumour-specific antigens was studied in two Marek’s disease lymphoblastoid cell lines (MSB-1 and HPRS-line 2).

The proportions of cells which spontaneously expressed virus antigens or which formed infective centres were considerably higher in MSB-1 than in HPRS-line 2, but did not exceed 10%. In contrast to virus antigens, thymus-specific and tumour-specific antigens were expressed on the majority of the cells. Treatment with IdUrd increased the proportion of cells forming infective centres in both cell lines and the proportion of cells expressing virus antigens in MSB-1. A decline in the proportion of cells forming infective centres in IdUrd-treated and in untreated cultures was noted during continuous subculture of both cell lines.

Direct evidence for the presence of virus-specific antigens in MSB-1 cells was obtained by immunodiffusion. The results suggested further that lymphoblastoid cells are unable to synthesize a major precipitating glycoprotein antigen (A antigen) normally associated with infection of permissive cells with MDV.

Analysis of surface proteins of normal thymus cells labelled by lactoperoxidase-catalysed iodination showed that thymus-specific determinants are associated with iodinated polypeptides in the mol. wt. range 45000 to 47000, 50000 to 58000 and 90000 to 150000. The major thymus-specific polypeptide exposed at the surface of normal thymus cells was in the mol. wt. range of 50000 to 58000. Surface proteins of lymphoblastoid cells were not accessible to iodination.

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1977-05-01
2022-01-18
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References

  1. Adams A., Strander H., Cantell K. 1975; Sensitivity of the Epstein-Barr virus transformed human lymphoid cell lines to interferon. Journal of General Virology 28:207–217
    [Google Scholar]
  2. Akiyama Y., Kato S., Iwa N. 1973; Continuous cell culture from lymphoma of Marek’s disease. Biken’s Journal 16:177–179
    [Google Scholar]
  3. Churchill A. E. 1968; Herpes-type virus isolated in cell culture from tumours of chickens with Marek’s disease. X. Studies in cell culture. Journal of the National Cancer Institute 41:939–950
    [Google Scholar]
  4. Churchill A. E., Chubb R. C., Baxendale W. 1969; The attenuation with loss of oncogenicity, of the herpes-type virus of Marek’s disease (strain HPRS-16) on passage in cell culture. Journal of General Virology 4:557–564
    [Google Scholar]
  5. Dimmock N. J., Watson D. H. 1969; Proteins specified by influenza virus in infected cells: analysis by polyacrylamide gel electrophoresis of antigens not present in the virus particle. Journal of General Virology 5:499–509
    [Google Scholar]
  6. Hampar B., Derge J. G., Nonoyama M., Chang S., Tagamets M. A., Showalter S. D. 1974; Programming of events in Epstein-Barr virus activated cells induced by 5-Iud R. Virology 62:71–89
    [Google Scholar]
  7. Hudson L., Payne L. N. 1973; An analysis of the T and B cells of Marek’s disease lymphomas of the chicken. Nature New Biology 241:52–53
    [Google Scholar]
  8. Hynes R. O. 1973; Alteration of cell surface proteins by viral transformation and by proteolysis. Proceedings of the National Academy of Sciences of the United States of America 70:3170–3174
    [Google Scholar]
  9. Kato S., Akiyama Y. 1975; Lymphoid cell lines from lymphomas of Marek’s disease. In Oncogenesis and Herpesviruses vol II pp 101–108 Edited by de The G., Epstein M. A., zur Hausen H. Lyon: International Agency for Research on Cancer; Publication No. 11
    [Google Scholar]
  10. Keski-Oja J., Vaheri A., Ruoslahti E. 1976; Fibroblast surface antigen (SF): The external glycoprotein lost in proteolytic stimulation and malignant transformation. International Journal of Cancer 17:261–269
    [Google Scholar]
  11. Matsuda H., Ikuta K., Kato S. 1976; Detection of T cell surface determinants in three Marek’s disease lymphoblastoid cell lines. Biken’s Journal 19:29–32
    [Google Scholar]
  12. Natt M. P., Herrick C. A. 1952; A new blood diluent for counting the erythrocytes and leucocytes of the chicken. Poultry Science 31:735–738
    [Google Scholar]
  13. Nazerian K. 1975; Induction of an early antigen of Marek’s disease virus in a lymphoblastoid cell line. In Oncogenesis and Herpesviruses vol II pp 345–350 Edited by de Thé G., Epstein G. M. A., zur Hausen H. Lyon: International Agency for Research on Cancer; Publication No. 11
    [Google Scholar]
  14. Nazerian K., Lee L. F. 1974; Deoxyribonucleic acid of Marek’s disease virus in lymphoblastoid cell lines from Marek’s disease tumours. Journal of General Virology 25:317–321
    [Google Scholar]
  15. Nyormoi O., Klein G., Adams A., Dombos L. 1973; Sensitivity to EBV superinfection and IudR inducibility of hybrid cells formed between a sensitive and a relatively resistant Burkitt lymphoma cell line. International Journal of Cancer 12:396–408
    [Google Scholar]
  16. Payne L. N., Powell P. C., Rennie M. 1974; Response of B and T lymphocytes and other blood leukocytes in chickens with Marek’s disease. Cold Spring Harbor Symposium on Quantitative Biology 39:817–826
    [Google Scholar]
  17. Payne L. N., Rennie M. 1970; Lack of effect of bursectomy on Marek’s disease. Journal of the National Cancer Institute 45:387–397
    [Google Scholar]
  18. Pearlstein E., Hynes R. O., Franks L. M., Hemmings V. J. 1976; Surface proteins and fibrinolytic activity of cultured mammalian cells. Cancer Research 36:1475–1485
    [Google Scholar]
  19. Powell P. C., Payne L. N., Frazier J. A., Rennie M. 1974; T lymphoblastoid cell lines from Marek’s disease lymphomas. Nature, London 251:79–80
    [Google Scholar]
  20. Powell P. C., Payne L. N., Frazier J. A., Rennie M. 1975; T-lymphoblastoid cell lines from Marek’s disease lymphomas. In Oncogenesis and Herpesviruses vol 11 pp 89–99 Edited by de The G., Epstein M. A., zur Hausen H. Lyon: International Agency for Research on Cancer; Publication No. 11
    [Google Scholar]
  21. Purchase H. G., Biggs P. M. 1967; Characterization of five isolates of Marek’s disease. Research in Veterinary Science 8:440–449
    [Google Scholar]
  22. Roblin R., Chou I., Black P. H. 1975; Proteolytic enzymes cell surface changes and viral transformation. In Advances in Cancer Research vol 22 pp 203–260 Edited by Klein G., Weizhouse S., Had-dow A. New York, London: Academic Press;
    [Google Scholar]
  23. Ross L. J. N. 1974; Comparison of antigenic glycoproteins and glycoprotein receptors of concanavalin A isolated from duck embryo cells infected with Marek’s disease virus and a herpesvirus of turkeys (strain FC 126). Journal of General Virology 24:549–562
    [Google Scholar]
  24. Ross L. J. N., Bassarab O., Walker D. J., Whitby B. 1975; Serological relationship between a pathogenic strain of Marek’s disease virus, its attenuated derivative and herpesvirus of turkeys. Journal of General Virology 28:37–47
    [Google Scholar]
  25. Ross L. J. N., Biggs P. M., Newton A. A. 1973; Purification and properties of the ‘A’ antigen associated with Marek’s disease virus infections. Journal of General Virology 18:291–304
    [Google Scholar]
  26. Rouse B. T., Wells R. J. H., Warner M. L. 1973; Proportion of T and B lymphocytes in lesions of Marek’s disease: theoretical implications for pathogenesis. Journal of Immunology no:534–539
    [Google Scholar]
  27. Stillstrom J. 1963; Grain count corrections in autoradiography. International Journal of Applied Radiation 14:113–118
    [Google Scholar]
  28. Watkins J. F. 1974; The SV40 rescue problem. Cold Spring Harbor Symposium on Quantitative Biology 39:355–362
    [Google Scholar]
  29. Westmoreland D., Watkins J. F. 1974; The IgG receptor induced by herpes simplex virus: studies using radioiodinated IgG. Journal of General Virology 24:167–178
    [Google Scholar]
  30. Westmoreland D., Watkins J. F., Rapp F. 1974; Demonstration of a receptor for IgG in Syrian Hamster cells transformed with herpes simplex virus. Journal of General Virology 25:167–170
    [Google Scholar]
  31. Witter R. L., Nazerian K., Purchase H. G., Burgoyne G. H. 1970; Isolation from turkeys of a cell-associated herpesvirus antigenically related to Marek’s disease virus. American Journal of Veterinary Research 31:525–538
    [Google Scholar]
  32. Witter R. L., Solomon J. J., Champion L. R., Nazerian K. 1975; Demonstration of a tumor-associated surface antigen in Marek’s disease. Journal of Immunology 115:177–183
    [Google Scholar]
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