Rapid Diagnosis of Rotavirus Infections: Comparison of Electron Microscopy and Immunoelectro-osmophoresis for the Detection of Rotavirus in Human Infantile Gastroenteritis Free

Abstract

SUMMARY

Eighty-seven faecal samples from infants and children suffering from acute gastroenteritis were investigated for the presence of rotavirus by immunoelectro-osmophoresis (IEOP) and electron microscopy (EM). Sixty-one % of the samples contained rotavirus antigens when examined by IEOP whereas only 50% were diagnosed as positive by EM. However, where it was possible to perform EM within the same day that the sample was received it took 24 h to establish the diagnosis by IEOP. The high sensitivity of the IEOP method was achieved by application of antiserum produced in rabbits to rotavirus immunoprecipitates. The specificity and sensitivity of the diagnostic antiserum produced were tested by application of different immunoelectrophoretic methods.

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1977-05-01
2024-03-29
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References

  1. Albrey M. B., Murphy A. M. 1976; Rotavirus growth in bovine monolayers. Lancet 1:753
    [Google Scholar]
  2. Albrey M. B., Murphy A. M., Path F. R. C. 1976; Rotavirus and acute gastroenteritis of infants and children. Medical Journal of Australia 1:82–85
    [Google Scholar]
  3. Banatvala J. E., Totterdell B., Chrystie I. L., Woode G. N. 1975; In vitro detection of human rotaviruses. Lancet 11:821
    [Google Scholar]
  4. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. 1973; Evidence for viral gastroenteritis. New England Journal of Medicine 389:1096–1097
    [Google Scholar]
  5. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. 1974; Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet i:149–151
    [Google Scholar]
  6. Flewett T. H., Bryden A. S., Davies H. 1973; Virus particles in gastroenteritis. Lancet ii:1497
    [Google Scholar]
  7. Flewett T. H., Bryden A. S., Davies H., Woode G. N., Bridger J. C., Derrick J. M. 1974; Relation between viruses from acute gastroenteritis of children and newborn calves. Lancet ii:61–63
    [Google Scholar]
  8. Grauballe P. C., Vestergaard B. F., Hornsleth A., Leerhoy J., Johnsson T. 1975; Demonstration by immunoelectro-osmophoresis of precipitating antibodies to a purified rubella virus antigen. Infection and Immunity 12:55–61
    [Google Scholar]
  9. Just Svendsen P. 1973; Fused rocket immunoelectrophoresis. Scandinavian Journal of Immunology 2: suppl 169–70
    [Google Scholar]
  10. Kapikian A. Z., Kim H. W., Wyatt R. G., Rodriguez W. J., Cline W. L., Parrott R. H., Chanock R. M. 1974; Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests. Science 185:1049–1053
    [Google Scholar]
  11. Kapikian A. Z., Cline W. L., Mebus C. A., Wyatt R. G., Kalica A. R., James H. D., Van Kirk D., Chanock R. M., Kim H. W. 1975; New complement-fixation test for the human reovirus-like agent of infantile gastroenteritis. Lancet i:1056–1061
    [Google Scholar]
  12. Kapikian A. Z., Kalica A. R., Shih J. W., Cline W. L., Thornhill T. S., Wyatt R. G., Chanock R. M., Kim H. W., Gerin J. L. 1976; Buoyant density in cesium chloride of the human reoviruslike agent of infantile gastroenteritis by ultracentrifugation, electron microscopy and complement fixation. Virology 70:564–569
    [Google Scholar]
  13. Kroll J. 1973; Line immunoelectrophoresis. Scandinavian Journal of Immunology 2: suppl 161–67
    [Google Scholar]
  14. Leading Article 1975a; Rotaviruses of man and animals. Lancet i:257–259
    [Google Scholar]
  15. Leading Article 1975b; Virus of infantile gastroenteritis. British Medical Journal 3:555–556
    [Google Scholar]
  16. Leading Article 1976; Recent advances in aetiology of enteritis in children. Medical Journal of Australia 1:75–76
    [Google Scholar]
  17. Middleton P. J., Szymanski M. T., Abbott G. D., Bortolussi R., Hamilton J. R. 1974; OrbivirUS acute gastroenteritis of infancy. Lancet i:1241–1244
    [Google Scholar]
  18. Middleton P. J., Petric M., Hewitt C. M., Szymanski M. T., Tam J. S. 1976; Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody. Journal of Clinical Pathology 39:191–197
    [Google Scholar]
  19. Orstavik I., Figenshau K. J., Haug K. W., Ulstrup J. C. 1976; A reovirus-like agent (rotavirus) in gastroenteritis of children. Virus detection and serological studies. Scandinavian Journal of Infectious Diseases 8:1–5
    [Google Scholar]
  20. Peterson M. W., Spendlove R. S., Smart R. A. 1976; Detection of neonatal calf diarrhoea virus, infant reoviruslike diarrhea virus, and coronavirus using the fluorescent virus precipitin test. Journal of Clinical Microbiology 3:376–377
    [Google Scholar]
  21. Purdham D. R., Purdham P. A., Evans N., Mcnaish A. S. 1975; Isolation of human rotavirus using human gut monolayers. Lancet ii:977
    [Google Scholar]
  22. Rodger S. M., Schnagl R. D., Holmes I. H. 1975; Biochemical and biophysical characteristics of diarrhea viruses of human and calf origin. Journal of Virology 16:1229–1235
    [Google Scholar]
  23. Spence L., Fauvel M., Bouchard S., Babiuk L., Saunders J. R. 1975; Test for reovirus-like agent. Lancet ii:322
    [Google Scholar]
  24. Tufvesson B., Johnsson T. 1976a; Occurrence of reo-like viruses in young children with acute gastroenteritis. Diagnosis established by electron microscopy and complement fixation, using the reo-like calf virus as antigen. Acta Pathologica et Microbiologica Scandinavica B 84:22–28
    [Google Scholar]
  25. Tufvesson B., Johnsson T. 1976b; Immunoelectro-osmophoresis for detection of reo-like virus: methodology and comparison with electron microscopy. Acta Pathologica et Microbiologia Scandinavica 84:225–228
    [Google Scholar]
  26. Vestergaard B. F. 1973; Crossed immunoelectrophoretic characterization of herpesvirus hominis type 1 and 2 antigens. Acta Pathologica et Microbiologica Scandinavica B 81:808–810
    [Google Scholar]
  27. Vestergaard B. F. 1975; Production of antiserum against a specific herpes simplex virus type 2 antigen. Scandinavian Journal of Immunology 4: suppl 2203–206
    [Google Scholar]
  28. Vestergaard B. F., Grauballe P. C. 1975; Separation and identification of herpes simplex virus antigens by ion-exchange chromatography and fused rocket immunoelectrophoresis. Scandinavian Journal of Immunology 4: suppl 2207–210
    [Google Scholar]
  29. Weeke B. 1973; Crossed immunoelectrophoresis. Scandinavian Journal of Immunology 2: suppl 147–56
    [Google Scholar]
  30. Wyatt R. G., Kapikian A. Z., Thornhill T. S., Sereno M. M., Kim H. W., Chanock R. M. 1974; In vitro cultivation in human foetal intestinal organ culture of a reovirus-like agent associated with nonbacterial gastroenteritis in infants and children. Journal of Infectious Diseases 130:523–528
    [Google Scholar]
  31. Wyatt R. G., Gill V. W., Sereno M. M., Kalica A. R., Van Kirk D. H., Chanock R. M., Kapikian A. Z. 1976; Probable in vitro cultivation of human reovirus-like agent of infantile diarresa. Lancet i:98–99
    [Google Scholar]
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