1887

Abstract

SUMMARY

Permeation chromatography on controlled pore glass (CPG) was compared with sucrose density gradient centrifugation for the purification of fifteen widely differing plant viruses. CPG chromatography gave excellent recovery of nearly all the viruses as evaluated by , serological titre and infectivity assay and proved as good as or better than sucrose density gradient centrifugation. Both methods gave similar separations of virus from host material and acceptably pure virus preparations. CPG columns accepted virus in high or low concentration; non-specific adsorption was not observed using CPG pre-treated with poly(ethylene glycol) of mol. wt. 20000.

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/content/journal/jgv/10.1099/0022-1317-35-1-77
1977-04-01
2024-04-24
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References

  1. Andrews P. 1965; The gel-filtration behaviour of proteins related to their molecular weights over a wide range. Biochemical Journal 96:595–606
    [Google Scholar]
  2. Gschwender H. H., Haller W., Hofschneider P. H. 1969; Large scale preparation of viruses by steric chromatography on columns of controlled pore glass: ϕ X 174, M13, M12, Qβ and T4 bacteriophages. Biochimica et Biophysica Acta 190:460–469
    [Google Scholar]
  3. Haller W. 1965; Chromatography on glass of controlled pore size. Nature, London 206:693–696
    [Google Scholar]
  4. Haller W. 1967; Virus isolation with glass of controlled pore size: MS-2 bacteriophage and Kilham virus. Virology 33:740–743
    [Google Scholar]
  5. Hawk G. L., Cameron J. A., Dufault L. B. 1972; Chromatography of biological materials on polyethylene glycol treated controlled pore glass. Preparative Biochemistry 2:193–203
    [Google Scholar]
  6. Hiatt C. W., Shelokov A., Rosenthal E. J., Galimore J. M. 1971; Treatment of controlled pore glass with polyfethylene oxide) to prevent adsorption of rabies virus. Journal of Chromatography 56:363–364
    [Google Scholar]
  7. Hollings M., Stone O. M., Barton R. J. 1977; Pathology, soil transmission and characterisation of cym- bidium ringspot, a virus from cymbidium orchids and white clover (Trifolium repens). Annals of Applied Biology (in the press)
    [Google Scholar]
  8. Marcinka K. 1972; Application of permeation chromatography on controlled pore glass in the purification of plant viruses. Acta Virologica 16:53–62
    [Google Scholar]
  9. Musil M. 1969; Red clover necrotic mosaic virus, a new virus infecting red clover (Trifolium pratense) in Czechoslovakia. Bioldgia, Bratislava 24:33–45
    [Google Scholar]
  10. Porath J. 1963; Some recently developed fractionation procedures and their application to peptide and protein hormones. Journal of Applied Chemistry 6:233–244
    [Google Scholar]
  11. Porath L., Flodin P. 1959; Gel filtration: a method for desalting and group separation. Nature, London 183:1657–1659
    [Google Scholar]
  12. Steere R. L. 1963; Tobacco mosaic virus: purifying and sorting associated particles according to length. Science 140:1089–1090
    [Google Scholar]
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