1887

Abstract

SUMMARY

The isolation of YS, a spontaneous variant of the CAM strain of tobacco rattle virus, is described. Unlike CAM, YS produced yellow symptoms in several species of host plant. In experiments with mixtures of virus particles from the two strains, this character was shown to be controlled by RNA-2. However, the coat proteins of YS and CAM were indistinguishable by serology, by electrophoresis in polyacrylamide gels or by tryptic peptide mapping. It is concluded that the mutation responsible for the yellow symptoms is not in the coat protein gene, but elsewhere in RNA-2.

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/content/journal/jgv/10.1099/0022-1317-35-1-37
1977-04-01
2024-12-12
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References

  1. Cooper J. I., Mayo M. A. 1972; Some properties of the particles of three tobravirus isolates. Journal of General Virology 16:285–297
    [Google Scholar]
  2. Duesberg P. H., Rueckert R. R. 1965; Preparative zone electrophoresis of proteins on polyacrylamide gels in 8 m urea. Analytical Biochemistry 11:342–361
    [Google Scholar]
  3. Ghabrial S. A., Lister R. M. 1973; Coat protein and symptom specification in tobacco rattle virus. Virology 52:1–12
    [Google Scholar]
  4. Harrison B. D. 1970; Tobacco rattle virus. C.M.I./A.A.B. Descriptions of Plant Viruses No 12:4 pp
    [Google Scholar]
  5. Harrison B. D., Nixon H. L. 1959; Separation and properties of particles of tobacco rattle virus with different lengths. Journal of General Microbiology 21:569–581
    [Google Scholar]
  6. Harrison B. D., Woods R. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610–620
    [Google Scholar]
  7. Lister R. M., Bracker C. E. 1969; Defectiveness and dependence in three related strains of tobacco rattle virus. Virology 37:262–275
    [Google Scholar]
  8. Mayo M. A., Robinson D. J. 1975; Revision of estimates of the molecular weights of tobravirus coat proteins. Intervirology 5:313–318
    [Google Scholar]
  9. Robinson D. J. 1973a; Inactivation and mutagenesis of tobacco rattle virus by nitrous acid. Journal of General Virology 18:215–222
    [Google Scholar]
  10. Robinson D. J. 1973b; Properties of two temperature–sensitive mutants of tobacco rattle virus. Journal of General Virology 21:499–506
    [Google Scholar]
  11. Sänger H. L. 1968; Characteristics of tobacco rattle virus. I. Evidence that its two particles are functionally defective and mutually complementing. Molecular and General Genetics 101:346–367
    [Google Scholar]
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