1887

Abstract

Summary

Recovery and specific infectivity of infectious RNA from cowpea chlorotic mottle virus of low specific infectivity (14 to 21 day infections) were greatly improved by using antioxidants during virus purification and RNA extraction, and by disrupting coat protein with pronase before phenol-SDS extraction. Total infectivity of RNA from virus of low infectivity was increased over 30 times. RNA profiles obtained using polyacrylamide gels were then similar for virus with high (4 to 7 day infections) or low specific infectivity. Low specific infectivity, therefore, seems to be caused by alteration of the coat protein or of the protein-RNA interaction in intact virus particles.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-35-1-175
1977-04-01
2019-10-23
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-35-1-175
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error