Infection of Tobacco Mesophyll Protoplasts with Raspberry Ringspot Virus alone and together with Tobacco Rattle Virus Free

Abstract

SUMMARY

Raspberry ringspot virus (RRV) infected tobacco mesophyll protoplasts best, judged by the yield of infective virus, when the inoculation mixture contained 0.04 to 5 g/ml virus, 1.0 to 1.5 g/ml poly--ornithine and phosphate buffer (0.006 to 0.025 , pH 6.0 to 9.0); without poly--ornithine no infection occurred. The optimum temperature for accumulation of infective virus was 20 to 22 °C, and the virus content reached about 2 × 10 particles/protoplast in 3 days at 22 °C. On staining with fluorescent antibody to purified virus particles, infected protoplasts gave a faint generalized cytoplasmic fluorescence, with many also containing a more brightly fluorescing spot thought to correspond with the vesiculated inclusion body found by electron microscopy.

In contrast, many of the protoplasts inoculated with mixtures of RRV and the CAM strain of tobacco rattle virus (TRV-CAM) contained numerous discrete granules of RRV particle antigen throughout the cytoplasm. Infection with RRV had little effect on the distribution of TRV-CAM particle antigen or on the proportion of protoplasts infected with TRV-CAM. Up to 95% of protoplasts became infected with both viruses, which apparently infected independently. The aggregates of RRV antigen formed only in protoplasts also infected with TRV-CAM.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-35-1-125
1977-04-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/35/1/JV0350010125.html?itemId=/content/journal/jgv/10.1099/0022-1317-35-1-125&mimeType=html&fmt=ahah

References

  1. Barker H., Harrison B. D. 1977; The interaction between raspberry ringspot and tobacco rattle viruses in doubly infected protoplasts. Journal of General Virology 35:135–145
    [Google Scholar]
  2. Cooper J. L., Mayo M. A. 1972; Some properties of the particles of three tobravirus isolates. Journal of General Virology 16:285–297
    [Google Scholar]
  3. De Zoeten G. A., Assink A. M., Van Kammen A. 1974; Association of cowpsa mosaic virus-induced doublestranded RNA with a cytopathological structure in infected cells. Virology 59:341–355
    [Google Scholar]
  4. Harrison B. D. 1958; Further studies on raspberry ringspot and tomato black ring, soil-borne viruses that affect raspberry. Annals of Applied Biology 46:571–584
    [Google Scholar]
  5. Harrison B. D., Kubo S., Robinson D. J., Hutcheson A. M. 1976; The multiplication cycle of tobacco rattle virus in tobacco mesophyll protoplasts. Journal of General Virology 33:237–248
    [Google Scholar]
  6. Harrison B. D., Murant A. F., Mayo M. A., Roberts I. M. 1974; Distribution of determinants for symptom production, host range and nematode transmissibility between the two RNA components of raspberry ringspot virus. Journal of General Virology 22:233–247
    [Google Scholar]
  7. Harrison B. D., Woods R. D. 1966; Serotypes and particle dimensions of tobacco rattle viruses from Europe and America. Virology 28:610–620
    [Google Scholar]
  8. Hibi T., Rezelman G., Van Kammen A. 1975; Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64:308–318
    [Google Scholar]
  9. Kubo S., Harrison B. D., Barker H. 1975b; Defined conditions for growth of tobacco plants as sources of protoplasts for virus infection. Journal of General Virology 28:255–257
    [Google Scholar]
  10. Kubo S., Harrison B. D., Robinson D. J. 1974; Effect of phosphate on the infection of tobacco protoplasts by tobacco rattle virus. Intervirology 3:382–387
    [Google Scholar]
  11. Kubo S., Harrison B. D., Robinson D. J., Mayo M. A. 1975a; Tobacco rattle virus in tobacco mesophyll protoplasts: infection and virus multiplication. Journal of General Virology 27:293–304
    [Google Scholar]
  12. Kubo S., Robinson D. J., Harrison B. D., Hutcheson A. M. 1976; Uptake of tobacco rattle virus by tobacco protoplasts, and the effect of phosphate on infection. Journal of General Virology 30:287–298
    [Google Scholar]
  13. Murant A. F., Mayo M. A., Harrison B. D., Goold R. A. 1972; Properties of virus and RNA components of raspberry ringspot virus. Journal of General Virology 16:327–338
    [Google Scholar]
  14. Otsuki Y., Takebe L. 1976; Double infection of isolated tobacco mesophyll protoplasts by unrelated plant viruses. Journal of General Virology 30:309–316
    [Google Scholar]
  15. Takebe I. 1975; The use of protoplasts in plant virology. Annual Review of Phytopathology 13:105–125
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-35-1-125
Loading
/content/journal/jgv/10.1099/0022-1317-35-1-125
Loading

Data & Media loading...

Most cited Most Cited RSS feed