1887

Abstract

SUMMARY

Herpes simplex virus (HSV) DNA molecules were isolated from infected BSC 1 cells and centrifuged in CsCl-ethidium bromide density gradients. Both newly labelled and mature virus DNA molecules were found to have a linear conformation. The morphology of virus DNA molecules at different stages of the virus growth cycle in BSC 1 cells, was studied by electron microscopy after separation of virus DNA from cellular DNA by centrifugation in CsCl gradients. In each sample, about 200 virus DNA molecules were photographed and the different morphological forms were studied. Four classes of virus DNA molecules were observed: () mature linear DNA molecules, 52.4 ± 3.3 µm in length, () DNA molecules that contain a replicative loop or are Y-shaped, resembling replicative intermediates, () virus DNA molecules having one or more single-stranded filaments attached to them and () molecules with collapsed regions or with branches. A few circular molecules as well as linear DNA molecules longer than unit length were also observed. The virus DNA molecules resembling replicative intermediates gradually increased in number and reached a maximal amount of about 5% of the virus DNA population at 12 h after infection. The other forms of virus DNA were found to persist after the number of replicating DNA molecules decreased.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-34-3-507
1977-03-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/34/3/JV0340030507.html?itemId=/content/journal/jgv/10.1099/0022-1317-34-3-507&mimeType=html&fmt=ahah

References

  1. Adams A., Lindahl T. 1975; Epstein-Barr virus genomes with properties of circular DNA molecules in carrier cells. Proceedings of the National Academy of Sciences of the United States of America 72:1477–1481
    [Google Scholar]
  2. Becker Y., Asher Y. 1975; In vitro synthesis of DNA in nuclei isolated from herpes simplex virus-infected cells, untreated and treated with metabolic inhibitors. Virology 63:209–220
    [Google Scholar]
  3. Ben-Porat T., Kaplan A. S., Stehn B., Rubenstein A. S. 1976; Concatemeric forms of intracellular herpes-virus DNA. Virology 69:547–560
    [Google Scholar]
  4. Broker T. 1973; An electron microscopic analysis of pathways for bacteriophage T4 DNA recombination. Journal of Molecular Biology 81:1–16
    [Google Scholar]
  5. Broker T. R., Lehman I. R. 1971; Branched DNA molecules: intermediates in T4 recombination. Journal of Molecular Biology 60:131–149
    [Google Scholar]
  6. Dressler D. 1975; The recent excitement in the DNA growing point problem. Annual Review of Microbiology 29:525–559
    [Google Scholar]
  7. Ellens D. J., Sussenbach J. S., Jansz H. S. 1974; Studies on the mechanism of replication of adenovirus DNA. III. Electron microscopy of replicating DNA. Virology 61:427–442
    [Google Scholar]
  8. Hayward G. S., Jacob R. J., Wadsworth S. C., Roizman B. 1975; Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientations of their long and short components. Proceedings of the National Academy of Sciences of the United States of America 72:4243–4247
    [Google Scholar]
  9. Holloman W. K., Wiegand R., Hoessli C., Radding C. M. 1975; Uptake of homologous single-stranded fragments by superhelical DNA: a possible mechanism for initiation of genetic recombination. Proceedings of the National Academy of Sciences of the United States of America 72:2394–2398
    [Google Scholar]
  10. Hudson B., Upholt W. B., Devinny J., Vinograd J. 1969; The use of an ethidium analogue in the dye-buoyant density procedure for the isolation of closed circular DNA: the variation of the superhelix density of mitochondrial DNA. Proceedings of the National Academy of Sciences of the United States of America 62:813–820
    [Google Scholar]
  11. Kiger J. A., Sinsheimer R. L. 1969; Vegetative lambda DNA. IV. Fractionation of replicating lambda DNA on benzoylated naphthoylated DEAE cellulose. Journal of Molecular Biology 40:467–490
    [Google Scholar]
  12. Kleinschmidt A. K., Dunnebacke T. H., Spendlove R. S., Schaffer F. L., Whitcomb R. F. 1964; Electron microscopy of RNA from reovirus and wound tumour virus. Journal of Molecular Biology 10:282–288
    [Google Scholar]
  13. Kleinschmidt A. K., Lang D., Jackerts D., Zahn R. K. 1962; Darstellung und langenmessungen des gesamten desoxyribonucleinaureinhaltes von T2-bakteriophagen. Biochimica et Biophysica Acta 61:857–864
    [Google Scholar]
  14. Pearson G. D. 1975; Intermediate in adenovirus type 2 replication. Journal of Virology 16:17–26
    [Google Scholar]
  15. Scudiero D., Strauss B. 1974; Accumulation of single-stranded regions in DNA and the block to replication in a human cell line alkylated with methyl methane sulfonate. Journal of Molecular Biology 83:17–34
    [Google Scholar]
  16. Sedat J. W., Kelley R. B., Sinsheimer R. L. 1967; Fractionation of nucleic acid on benzoylated napthoylated DEAE cellulose. Journal of Molecular Biology 2:537–540
    [Google Scholar]
  17. Sheldrick P., Berthelot N. 1975; Inverted repetitions in the chromosome of herpes simplex virus. Cold Spring Harbor Symposium on Quantitative Biology 39:667–678
    [Google Scholar]
  18. Shlomai J., Friedmann A., Becker Y. 1976a; Replicative intermediates of herpes simplex virus DNA. Virology 69:647–659
    [Google Scholar]
  19. Shlomai J., Strauss B., Asher Y., Friedmann A., Becker Y. 1976b; Analysis of herpes virus DNA synthesized in infected nuclei by chromatography on benzoylated naphthoylated DEAE-celluIose columns. Journal of General Virology 32:189–204
    [Google Scholar]
  20. Subak-Sharpe J. H., Brown S. M., Ritchie D. A., Timbury M. C., Macnab J. C. M., Marsden H. S., Hay J. 1974; Genetic and biochemical studies with herpesvirus. Cold Spring Harbor Symposium on Quantitative Biology 39:717–730
    [Google Scholar]
  21. Sussenbach J. S., Ellens D. J., Jansz H. S. 1973; Studies on the mechanism of replication of adenovirus DNA. II. The nature of single-stranded DNA in replicative intermediates. Journal of Virology 12:1131–1138
    [Google Scholar]
  22. Tattersall R., Crawford L. V., Shatkin A. J. 1973; Replication of parvovirus MVM II. Isolation and characterization of intermediates in the replication of the viral DNA. Journal of Virology 12:1446–1456
    [Google Scholar]
  23. Wildy P. 1955; Recombination with herpes simplex virus. Journal of General Microbiology 13:34–46
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-34-3-507
Loading
/content/journal/jgv/10.1099/0022-1317-34-3-507
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error