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1887

Abstract

SUMMARY

Phenotypic expression of different genes controlling resistance to tobacco mosaic virus (TMV) in tomato was analysed in the protoplast system using otherwise isogenic breeding lines. Genes -2 and -2 were not expressed and did not prevent TMV-L, a common tomato strain of TMV, infecting and multiplying. By contrast, homozygous gene -1 was able to express its effect in protoplasts as well as in leaf discs; no virus progeny were detected by fluorescent antibody staining or by infectivity assay up to 3 days after inoculation with TMV-L. Protoplasts and leaf discs homozygous for -1, however, became infected with TMV-CH2, a tomato strain able to overcome the effects of -1 in intact plants.

Protoplasts from P. I. 128650, known to have a high level of resistance to TMV, were as readily infected with TMV-L, and synthesized progeny virus as rapidly as protoplasts from susceptible tomato. This genotype seems to have no resistance expressible in isolated protoplasts.

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/content/journal/jgv/10.1099/0022-1317-34-3-499
1977-03-01
2025-06-23
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References

  1. Alexander L. J. 1971; Host-pathogen dynamics of tobacco mosaic virus on tomato. Phytopathology 61:611–617
    [Google Scholar]
  2. Cirulli M., Alexander L. J. 1969; Influence of temperature and strain of tobacco mosaic virus on resistance in a tomato breeding line derived from Lycopersicon peruvianum. Phytopathology 59:1287–1297
    [Google Scholar]
  3. Dawson J. R. O. 1965; Contrasting effects of resistant and susceptible tomato plants on tomato mosaic virus multiplication. Annals of Applied Biology 56:485–491
    [Google Scholar]
  4. Dawson J. R. O. 1967; The adaptation of tomato mosaic virus to resistant tomato plants. Annals of Applied Biology 60:209–214
    [Google Scholar]
  5. Frazier W. A., Dennett R. K. 1949; Tomato lines of Lycopersicon esculentum type resistant to tobacco mosaic virus. Proceedings of the American Society for Horticultural Science 54:265–271
    [Google Scholar]
  6. Holmes F. O. 1954; Inheritance of resistance to infection by tobacco-mosaic virus in tomato. Phytopathology 44:640–642
    [Google Scholar]
  7. Holmes F. O. 1957; True-breeding resistance in tomato to infection by tobacco-mosaic virus. Phytopathology 47:16–17
    [Google Scholar]
  8. Kikuta K., Frazier W. A. 1947; Preliminary report on breeding tomatoes for resistance to tobacco mosaic virus. Proceedings of the American Society for Horticultural Science 49:256–262
    [Google Scholar]
  9. Mcritchie J. J., Alexander L. J. 1963; Host specific Lycopersicon strains of tobacco mosaic virus. Phytopathology 53:394–398
    [Google Scholar]
  10. Motoyoshi F., Bancroft J. B., Watts J. W. 1974; The infection of tobacco protoplasts with a variant of brome mosaic virus. Journal of General Virology 25:31–36
    [Google Scholar]
  11. Motoyoshi F., Oshima N. 1975; Infection with tobacco mosaic virus of leaf mesophyll protoplasts from susceptible and resistant lines of tomato. Journal of General Virology 29:81–91
    [Google Scholar]
  12. Motoyoshi F., Oshima N. 1976; The use of tris–HCl buffer for inoculation of tomato protoplasts with tobacco mosaic virus. Journal of General Virology 32:311–314
    [Google Scholar]
  13. Nozu Y., Okada Y. 1968; Amino acid sequence of a common Japanese strain of tobacco mosaic virus. Journal of Molecular Biology 35:643–646
    [Google Scholar]
  14. Oshima N. 1973; Classification of strains of tobacco mosaic virus by cross protection tests. Report of the Tottori Mycological Institute (Japan) No. 10 771–778
    [Google Scholar]
  15. Oshima N., Goto T., Sato R. 1964; A strain of tobacco mosaic virus (TMV-L) isolated from tomato. Research Bulletin No. 83 of the Hokkaido National Agricultural Experimental Station87–99
    [Google Scholar]
  16. Oshima N., Ogiwara S., Aoki H. 1971a; Tomato strain of TMV isolated from resistant lines of tomato. Annals of the Phytopathological Society of Japan 37:401–402
    [Google Scholar]
  17. Oshima N., Ohashi Y., Umekawa M. 1971b; Studies of some strains of tobacco mosaic virus pathogenic to crucifer plants. I. Physical and chemical studies. Annals of the Phytopathological Society of Japan 37:319–325
    [Google Scholar]
  18. Otsuki Y., Shimomura T., Takebe I. 1972; Tobacco mosaic virus multiplication and expression of the N gene in necrotic responding tobacco varieties. Virology 50:45–50
    [Google Scholar]
  19. Otsuki Y., Takebe I. 1969; Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplasts. Virology 38:497–499
    [Google Scholar]
  20. Pelham J. 1966; Resistance in tomato to tobacco mosaic virus. Euphytica 15:258–267
    [Google Scholar]
  21. Pelham J. 1972; Strain-genotype interaction of tobacco mosaic virus in tomato. Annals of Applied Biology 71:219–228
    [Google Scholar]
  22. Pelham J., Fletcher J. T., Hawkins J. H. 1970; The establishment of a new strain of tobacco mosaic virus resulting from the use of resistant varieties of tomato. Annals of Applied Biology 65:293–297
    [Google Scholar]
  23. Soost R. K. 1963; Hybrid tomato resistant to tobacco mosaic virus. Journal of Heredity 54:241–244
    [Google Scholar]
  24. Takebe L. 1975a; The use of protoplasts in plant virology. Annual Review of Phytopathology 13:105–125
    [Google Scholar]
  25. Takebe L. 1975b; Protoplasts from leaf mesophyll and their infection by plant viruses. Review of Plant Protection Research 8:136–150
    [Google Scholar]
  26. Wilkinson R. E. 1969; Application of selection pressure to isolate mutants of tobacco mosaic virus adapted to tomato plants with the Tm1 gene for resistance. Phytopathology 59:404
    [Google Scholar]
  27. Zaitlin M., Beachy R. N. 1974; Protoplasts and separated cells in plant virus research. Advances in Virus Research 19:1–35
    [Google Scholar]
  28. Zitter T. A., Murakishi H. H. 1969; Nature of increased virulence in tobacco mosaic virus after passage in resistant tomato plants. Phytopathology 59:1736–1739
    [Google Scholar]
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