The specificity of guinea pig antisera against large cyanogen bromide-cleaved peptides of the virus capsid protein VP3 of foot-and-mouth disease virus type O1, strain Kaufbeuren has been characterized by double immunodiffusion, virus neutralization and protection tests. Antibodies to purified 146S particles and the cleavage peptides of VP3 showed an incomplete cross-section against VP3 peptide antigen when reacted in immunodiffusion tests, indicating that new antigenic determinants are exhibited by the peptides which are not recognized by the antiserum against the native virus proteins.
The immune response against the reduced, unfolded chain constituents of VP3 was lower in comparison to that of native virus particles but still some immunological determinants remained actively capable of inducing virus-neutralizing antibodies in immunized guinea pigs.
BachrachH. L.,
MooreD. M.,
MckercherP. D.,
PolatnickJ.1975; Immune and antibody responses to an isolated capsid protein of foot-and-mouth disease virus. The Journal of Immunology 115:1636–1641
BurroughsJ. N.,
RowlandsD. J.,
SangarD. J.,
TalbotP.,
BrownF.1971; Further evidence for multiple proteins in the foot-and-mouth disease virus particle. Journal of General Virology 13:73–84
LaporteJ.,
GrosclaudeJ.,
WantyghemJ.,
BernardS.,
RouzeP.1973; Neutralisation en culture cellulaire du pouvoir infectieux du virus de la fievre aphteuse par des serums provenant de pores immunisés a l’aide d’une proteine virale purifiee. Compte rendus Hebdomadaires des Seances de VAcademie des Sciences (Paris), serie d 276:3399–3402
StrohmaierK.,
AdamK.-H.1975; New results concerning the coat proteins of foot-and-mouth disease virus. Office international des epizooties. Bulletin 83:425–429
Vande WoudeG. F.,
SwaneyJ. B.,
BachrachH. L.1972; Chemical and physical properties of foot-and-mouth disease virus: a comparison with Maus-Elberfeld virus. Biochemical and Biophysical Research Communications 48:1222–1229