1887

Abstract

SUMMARY

Palisade parenchyma protoplasts isolated from the tobacco varieties Samsun and Samsun NN were inoculated with cowpea mosaic virus (CPMV). By fluorescent antibody staining it was demonstrated that about 75% of the protoplasts became infected. Poly--ornithine was required for infection. The cytopathic structures shown previously to be characteristic for CPMV-infected cowpea cells were also produced in CPMV-infected tobacco protoplasts. The optimum inoculum concentration for CPMV was 4 to 20 µg/ml. Compared with CPMV multiplication in cowpea protoplasts and tobacco mosaic virus multiplication in tobacco protoplasts, the growth of CPMV in tobacco protoplasts had a longer lag period but the virus concentrations in all three kinds of infection finally reached similar values. The difficulty with which tobacco leaves are infected with CPMV contrasts with the ease of infection of tobacco mesophyll protoplasts.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-34-2-315
1977-02-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/34/2/JV0340020315.html?itemId=/content/journal/jgv/10.1099/0022-1317-34-2-315&mimeType=html&fmt=ahah

References

  1. Aoki S., Takebe I. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus ribonucleic acid. Virology 39:439–448
    [Google Scholar]
  2. De Zoeten G. A., Assink A. M., Van Kammen A. 1974; Association of cowpea mosaic virus-induced doublestranded RNA with a cytopathological structure in infected cells. Virology 59:341–355
    [Google Scholar]
  3. Hibi T., Rezelman G., Van Kammen A. 1975; Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64:308–318
    [Google Scholar]
  4. Kubo S., Harrison B. D., Robinson D. J., Mayo M. A. 1975; Tobacco rattle virus in tobacco mesophyll protoplasts: infection and virus multiplication. Journal of General Virology 27:293–304
    [Google Scholar]
  5. Kubo S., Robinson D. J., Harrison B. D., Hutcheson A. M. 1976; Uptake of tobacco rattle virus by tobacco protoplasts, and the effect of phosphate on infection. Journal of General Virology 30:287–298
    [Google Scholar]
  6. Motoyoshi F., Bancroft J. B., Watts J. W., Burgess J. 1973; The infection of tobacco protoplasts with cowpea chlorotic mottle virus and its RNA. Journal of General Virology 20:177–193
    [Google Scholar]
  7. Motoyoshi F., Bancroft J. B., Watts J. W. 1974; The infection of tobacco mesophyll protoplasts with a variant of brome mosaic virus. Journal of General Virology 25:31–36
    [Google Scholar]
  8. Motoyoshi F., Hull R. 1974; The infection of tobacco protoplasts with pea enation mosaic virus. Journal of General Virology 24:89–99
    [Google Scholar]
  9. Motoyoshi F., Hull R., Flack I. H. 1975; Infection of tobacco mesophyll protoplasts by alfalfa mosaic virus. Journal of General Virology 27:263–266
    [Google Scholar]
  10. Otsuki Y., Takebe I. 1969; Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplasts. Virology 38:497–498
    [Google Scholar]
  11. Otsuki Y., Takebe I. 1973; Infection of tobacco mesophyll protoplasts by cucumber mosaic virus. Virology 52:433–438
    [Google Scholar]
  12. Otsuki Y., Takebe I., Honda Y., Kajita S., Matsui C. 1974; Infection of tobacco mesophyll protoplasts by potato virus x. Journal of General Virology 22:375–385
    [Google Scholar]
  13. Sugimura Y., Ushiyama R. 1975; Cucumber green mottle mosaic infection and its bearing on cytological alterations in tobacco mesophyll protoplasts. Journal of General Virology 29:93–98
    [Google Scholar]
  14. Swaans H., Van Kammen A. 1973; Reconsideration of the distinction between the severe and yellow strains of cowpea mosaic virus. Netherlands Journal of Plant Pathology 79:257–265
    [Google Scholar]
  15. Takebe I., Otsuki Y., Aoki S. 1969; Isolation of tobacco mesophyll cells in intact and active state. Plant and Cell Physiology (Tokyo) 9:115–124
    [Google Scholar]
  16. Takebe L., Otsuki Y. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proceedings of the National Academy of Science of the United States of America 64:843–848
    [Google Scholar]
  17. Van Kammen A. 1967; Purification and properties of the components of cowpea mosaic virus. Virology 31:633–642
    [Google Scholar]
  18. Zabel P., Weenen-Swaans H., Van Kammen A. 1974; In vitro replication of cowpea mosaic virus RNA. I. Isolation and properties of the membrane-bound replicase. Journal of Virology 14:1044–1053
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-34-2-315
Loading
/content/journal/jgv/10.1099/0022-1317-34-2-315
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error