Vesicular stomatitis virus (VSV) induces polykaryocytes in rat embryonic fibroblasts transformed by the Prague strain of Rous sarcoma virus (XC cells). The cell fusion requires the uncoating of the virus in the cell, the synthesis of normally structured G and M proteins and their incorporation into the cell membrane. The synthesis of fully infectious virus is unnecessary. In addition to these antigens, a special yet undefined constitution of the host membrane is also important. With thermosensitive mutants non-defective for G and M antigens, cell fusion is much more extensive at the non-permissive temperature (39.6°C) than at the permissive one (31°C). The importance of the two antigens is also shown using rifampicin-sensitive mutants. We postulate that these two antigens induce in the cell membrane an imbalance in the distribution of phospholipids which then diffuse through membrane junctions to surrounding cells, provoking thereafter the cell fusion.
AbrahamG.,
BanerjeeA. K.1976; Sequential transcription of the genes of vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 73:1504–1508
BallL. A.,
WhiteC. N.1976; Order of transcription of genes of vesicular stomatitis virus. Proceedings of the National Academy of Sciences of the United States of America 73:442–446
BrattM. A.,
GallaherW. R.1969; Preliminary analysis of the requirements for fusion from within and fusion from without by new castle disease virus. Proceedings of the National Academy of Sciences of the United States of America 64:536–543
ChanyC.,
CookM. J.1960; Sur un facteur induit par le virus entrainant la formation de syncytium enculture cellulaire. Annates de l’Institut Pasteur 98:920–924
Chany-FournierF.,
MoreauM. C.,
Valle SantosM.1976; Induction de polycaryocytes par le virus de la stomatite vesiculaire dans les cellules de rat XC. Compte Rendu Hebdomadaires des Séances de Académie des Sciences 282:2007–2010
FlamandA.1970; Étude genetique du virus de la stomatite vesiculaire: classement de mutants thermo-sensibles spontanés en groupes de complémentation. Journal of General Virology 8:187–195
FlamandA.,
LafayF.1973; Étude des mutants thermosensibles du virus de la stomatite vesiculaire appartenant au groupe de complémentation II. Annales de l’Institut Pasteur 124:261–269
HenleG.,
DeinhardtF.,
GirardiA.1954; Cytopathic effect of mumps virus in tissue cultures of epithelial cell. Proceedings of the Society for Experimental Biology and Medicine 87:381–393
LafayF.1969; Étude des mutants thermosensibles du virus de la stomatite vesiculaire (VSV): classement de quelques mutants d’après des critères de fonctionnement. Compte Rendu Hebdomaires des Séances de l’ Académie des Sciences 268:2385–2388
LafayF.1974; Envelope proteins of vesicular stomatitis virus: effect of temperature-sensitive mutants in complementation groups III and V. Journal of Virology 14:1220–1228
LafayF.1975 PhD. Thesis Les protéines de l’enveloppe du virus de la stomatite vésiculaire: effet des mutations thermosensibles des groupes de complémentation III et V Universite de Paris-Sud, Centre; d’Orsay, France:
LepineP.,
ChanyC.,
DrozB.,
Robbe-FossatF.1959; Cytopathogenic effect of two newly recognized myxovirus strains: mechanism of syncytial formation. Annales of the New York Academy of Sciences 81:62–72
MoreauM. C.,
SanzeyB.1977; Rifampin-susceptible mutant of vesicular stomatitis virus: protein and RNA synthesis. Journal of Virology 21: (in the press)
NishiyamaY.,
ItoY.,
SchimokataK.,
KimuraY.,
NagataI.1976; Polykaryocyte formation induced by VSV in mouse L cells. Journal of General Virology 32:85–96
PapahadjopoulosD.,
PosteG.,
SchaefferB. E.1973; Fusion of mammalian cells by unilamellar lipid vesicles: influence of lipid surface charge, fluidity and cholesterol. Biochimica et Biophysica Acta 323:23–42
PrintzP.,
WagnerR. R.1971; Temperature sensitive mutants of vesicular stomatitis virus: synthesis of virus-specific proteins. Journal of Virology 7:651–662
RepikP.,
FlamandA.,
BishopD. R. L.1974; Effect of interferon upon the primary and secondary transcription of vesicular stomatitis and influenza viruses. Journal of Virology 14:1169–1178
WagnerR. R.,
KileyM. P.,
SnyderR. M.,
SchnaitmanC. A.1972a; Cytoplasmic compartimentalization of the protein and ribonucleic acid species of vesicular stomatitis virus. Journal of Virology 9:672–683
WagnerR. R.,
PrevecL.,
BrownF.,
SummersD. F.,
SokolR.,
MacleodR.1972b; Classification of rhabdovirus proteins: a proposal. Journal of Virology 10:1228–1230
WagnerR. R.,
SnyderR. M.,
YamazakiS.1970; Proteins of vesicular stomatitis virus: kinetics and cellular sites of synthesis. Journal of Virology 5:548–558
WisnieskiB. J.,
ParkesJ. G.,
HuangY. O.,
FoxC. F.1974; Physical and physiological evidence for two phase transitions in cytoplasmic membranes of animal cells. Proceedings of the National Academy of Sciences of the United States of America 71:4381–4385