1887

Abstract

SUMMARY

The RNA from infectious pancreatic necrosis virus has been purified and had a sedimentation velocity of 14S on sucrose gradients, a buoyant density of 1.60 g/ml in CsSO and pyrimidine to purine ratios near unity. The RNA had the appearance of a linear double stranded molecule with an average length of 0.92 µm and a standard deviation of 0.07 µm when observed under the electron microscope using the Kleinschmidt protein film technique. This would correspond to a mol. wt. of 2.4±0.2 × 10. The RNase A resistance of IPN virus RNA exhibited a marked salt dependence; it was 92% resistant in 0.1 -NaCl, but only 9% resistant, or less, in 0.01 -NaCl. The RNA was resistant to denaturation by boiling at NaCl concentrations of 0.04 or higher, but did denature at lower concentrations. Polyacrylamide gel electrophoresis of the RNA indicated that two RNA species were present and the standard deviation of lengths in the electron microscope indicated that they could not differ by more than 4 × 10 in mol. wt.

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1977-02-01
2024-10-08
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References

  1. Bevan E. A., Herring A. J., Mitchell D. J. 1973; Preliminary characterization of two species of dsRNA in yeast and their relationship to the ‘killer’ character. Nature, London 245:81–86
    [Google Scholar]
  2. Bray G. A. 1960; A simple efficient liquid scintillator for counting aqueous solutions in a liquid scintillation counter. Analytical Biochemistry 1:279–285
    [Google Scholar]
  3. Buck K. W., Ratti G. 1975; Biophysical and biochemical properties of two viruses isolated from Aspergillus foetidus. Journal of General Virology 27:211–224
    [Google Scholar]
  4. Cohen J., Poinsard A., Scherrer R. 1973; Physico-chemical and morphological features of infectious pancreatic necrosis virus. Journal of General Virology 21:485–498
    [Google Scholar]
  5. Davis R. W., Simon-Martha R., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymology 21D:413–428
    [Google Scholar]
  6. Doty P., Mcgill-Barbara B., Rice S. A. 1958; The properties of sonic fragments of desoxribose nucleic acid. Proceedings of the National Academy of Sciences of the United States of America 44:432–438
    [Google Scholar]
  7. Gomatos P. J., Stoeckenius W. 1964; Electron microscope studies on reovirus RNA. Proceedings of the National Academy of Sciences of the United States of America 52:1449–1455
    [Google Scholar]
  8. Herring A. J., Bevan E. A. 1974; Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cerevisiae. Journal of General Virology 22:387–394
    [Google Scholar]
  9. Kavenoff K., Talcove D., Mudd J. A. 1975; Genome sized RNA from reovirus particles. Proceedings of the National Academy of Sciences of the United States of America 72:4317–4321
    [Google Scholar]
  10. Kelley R. K., Loh P. C. 1972; Electron microscopical and biochemical characterization of infectious pancreatic necrosis virus. Journal of Virology 10:824–834
    [Google Scholar]
  11. Kleinschmidt A. K. 1968; Monolayer techniques in electron microscopy of nucleic acid molecules. Methods in Enzymology 21B:361–377
    [Google Scholar]
  12. Langridge R., Gomatos P. J. 1963; The structure of RNA. Science 141:694–698
    [Google Scholar]
  13. Loening U. E. 1967; The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel-electrophoresis. Biochemical Journal 102:251–257
    [Google Scholar]
  14. Malsberger R. G., Cerini C. P. 1963; Characteristics of infectious pancreatic necrosis virus. Journal of Bacteriology 86:1283–1287
    [Google Scholar]
  15. Malsberger R. G., Cerini C. P. 1965; Multiplication of infectious pancreatic necrosis virus. Annals of the New York Academy of Sciences 126:555–565
    [Google Scholar]
  16. Moss L. H., Gravell M. 1969; Ultrastructure and sequential development of infectious pancreatic necrosis virus. Journal of Virology 3:52–58
    [Google Scholar]
  17. Nicholson B. L. 1971; Macromolecular synthesis in RTG-2 cells following infection with infectious pancreatic necrosis (IPN) virus. Journal of General Virology 13:369–372
    [Google Scholar]
  18. Wolf K., Quimby M. C., Bradford A. D. 1963; Egg-associated transmission of IPN virus of trouts. Virology 21:317–321
    [Google Scholar]
  19. Wolf K., Snieszko S. F., Dunbar C. E., Pyle E. 1960; Virus nature of infectious pancreatic necrosis in trout. Proceedings of the Society for Experimental Biology and Medicine 104:105–108
    [Google Scholar]
  20. Wood H. A., Bozarth R. F. 1972; Properties of virus-like particles of Penicillium chrysogenum: one double-stranded RNA molecule per particle. Virology 47:604–609
    [Google Scholar]
  21. Yamamoto T. 1975; Frequency of detection and survival of infectious pancreatic necrosis virus in a carrier population of Brook Trout (Salvelinus fontinalis) in a lake. Journal of Fisheries Research Board of Canada 32:568–570
    [Google Scholar]
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