The synthesis of herpes simplex virus DNA in isolated nuclei under conditions was found to be dependent on the addition of ATP and an ATP generating system to the reaction mixture. DNA synthesis was stimulated and prolonged when -hydroxymercuribenzoate was added to the isolated nuclei. Under these improved conditions virus DNA molecules which were initiated were completed , but most of the DNA molecules synthesized sedimented in sucrose gradients more slowly than herpes virion DNA. Denaturation of the labelled DNA molecules produced short single-stranded labelled DNA chains. Thus, under our improved conditions there was prolonged synthesis of DNA at a high rate, with the formation of both complete and incomplete virus DNA molecules.


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