@article{mbs:/content/journal/jgv/10.1099/0022-1317-34-1-37, author = "Tonew, E. and Fahlbusch, Bärbel", title = "Effects of Pyrimidine Derivatives on RNA-Dependent RNA Polymerase of Mengovirus-Infected Fogh and Lund (FL) cells", journal= "Journal of General Virology", year = "1977", volume = "34", number = "1", pages = "37-45", doi = "https://doi.org/10.1099/0022-1317-34-1-37", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-34-1-37", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "SUMMARY Two newly synthesized pyrimidine derivative were found to possess antiviral activity against Mengovirus in Fogh and Lund (FL) cells and in a cell-free system. The inhibitory effect on RNA-dependent RNA polymerase of Mengovirus-infected FL cells was assayed using 14C-UTP as precursor. Addition of 50 or 100 µM of the inhibitors in a cell-free system of crude enzyme and nucleoside triphosphate medium for 60 min incubation at 37 °C resulted in about 40 to 60% lower counting rates for drug-treated reaction mixtures. The analysis of the polymerase synthesis product (virus RNA extracted from the cell-free reaction mixture and deproteinized by the phenol-SDS method) was carried out by means of agarose-acrylamide gel electrophoresis. The main finding was a reduction of single-stranded Mengovirus RNA (RNase-sensitive and LiCl-precipitable). The rates of synthesis of the replicative intermediate (LiCl-precipitable) and the replicative form of RNA (LiCl-soluble) were not significantly influenced.", }